GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish

Detalhes bibliográficos
Autor(a) principal: Domingues, William B.
Data de Publicação: 2021
Outros Autores: Silveira, Tony L. R., Nunes, Leandro S., Blodorn, Eduardo B., Schneider, Augusto, Corcine, Carine D., Varela Junior, Antônio S., Acosta, Izani B., Kütter, Mateus T., Greif, Gonzalo, Robello, Carlos, Pinhal, Danillo [UNESP], Marins, Luís F., Campos, Vinicius F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fgene.2021.704778
http://hdl.handle.net/11449/229560
Resumo: Overexpression of growth hormone (GH) in gh-transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. It is hypothesized that microRNAs are directly involved in the regulation of fertility potential during spermatogenesis. The primary aim of our study was to verify whether gh overexpression disturbs the sperm miRNA profile and influences the sperm quality in transgenic zebrafish. We report a significant increase in body weight of gh-transgenic males along with associated reduced sperm motility and other kinetic parameters in comparison to the non-transgenic group. MicroRNA transcriptome sequencing of gh-transgenic zebrafish sperms revealed expressions of 186 miRNAs, among which six miRNA were up-regulated (miR-146b, miR-200a-5p, miR-146a, miR-726, miR-184, and miR-738) and sixteen were down-regulated (miR-19d-3p, miR-126a-5p, miR-126b-5p, miR-22a-5p, miR-16c-5p, miR-20a-5p, miR-126b-3p, miR-107a-3p, miR-93, miR-2189, miR-202–5p, miR-221–3p, miR-125a, miR-125b-5p, miR-126a-3p, and miR-30c-5p) in comparison to non-transgenic zebrafish. Some of the dysregulated miRNAs were previously reported to be related to abnormalities in sperm quality and reduced reproduction ability in other species. In this study, an average of 134 differentially expressed miRNAs-targeted genes were predicted using the in silico approach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that the genes of affected pathways were primarily related to spermatogenesis, sperm motility, and cell apoptosis. Our results suggested that excess GH caused a detrimental effect on sperm microRNAome, consequently reducing the sperm quality and reproductive potential of zebrafish males.
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spelling GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic ZebrafishDanio rerioepigeneticmiRNA-seqnon-coding RNAssperm motilitytransgenic fishOverexpression of growth hormone (GH) in gh-transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. It is hypothesized that microRNAs are directly involved in the regulation of fertility potential during spermatogenesis. The primary aim of our study was to verify whether gh overexpression disturbs the sperm miRNA profile and influences the sperm quality in transgenic zebrafish. We report a significant increase in body weight of gh-transgenic males along with associated reduced sperm motility and other kinetic parameters in comparison to the non-transgenic group. MicroRNA transcriptome sequencing of gh-transgenic zebrafish sperms revealed expressions of 186 miRNAs, among which six miRNA were up-regulated (miR-146b, miR-200a-5p, miR-146a, miR-726, miR-184, and miR-738) and sixteen were down-regulated (miR-19d-3p, miR-126a-5p, miR-126b-5p, miR-22a-5p, miR-16c-5p, miR-20a-5p, miR-126b-3p, miR-107a-3p, miR-93, miR-2189, miR-202–5p, miR-221–3p, miR-125a, miR-125b-5p, miR-126a-3p, and miR-30c-5p) in comparison to non-transgenic zebrafish. Some of the dysregulated miRNAs were previously reported to be related to abnormalities in sperm quality and reduced reproduction ability in other species. In this study, an average of 134 differentially expressed miRNAs-targeted genes were predicted using the in silico approach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that the genes of affected pathways were primarily related to spermatogenesis, sperm motility, and cell apoptosis. Our results suggested that excess GH caused a detrimental effect on sperm microRNAome, consequently reducing the sperm quality and reproductive potential of zebrafish males.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Genômica Estrutural Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de PelotasLaboratório de Biologia Molecular Instituto de Ciências Biológicas Universidade Federal do Rio GrandeFaculdade de Nutrição Universidade Federal de PelotasPrograma de Pós-Graduação em Veterinária Faculdade de Veterinária Universidade Federal de PelotasUnidad de Biología Molecular Institut PasteurLaboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP)Laboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP)CAPES: AUXPE #2537/2018FAPESP: FAPERGS FAPESP #19/2551-0000953-3Universidade Federal de PelotasUniversidade Federal do Rio GrandeInstitut PasteurUniversidade Estadual Paulista (UNESP)Domingues, William B.Silveira, Tony L. R.Nunes, Leandro S.Blodorn, Eduardo B.Schneider, AugustoCorcine, Carine D.Varela Junior, Antônio S.Acosta, Izani B.Kütter, Mateus T.Greif, GonzaloRobello, CarlosPinhal, Danillo [UNESP]Marins, Luís F.Campos, Vinicius F.2022-04-29T08:33:13Z2022-04-29T08:33:13Z2021-09-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fgene.2021.704778Frontiers in Genetics, v. 12.1664-8021http://hdl.handle.net/11449/22956010.3389/fgene.2021.7047782-s2.0-85115357322Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Geneticsinfo:eu-repo/semantics/openAccess2022-04-29T08:33:13Zoai:repositorio.unesp.br:11449/229560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:39.495928Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
title GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
spellingShingle GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
Domingues, William B.
Danio rerio
epigenetic
miRNA-seq
non-coding RNAs
sperm motility
transgenic fish
title_short GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
title_full GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
title_fullStr GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
title_full_unstemmed GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
title_sort GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish
author Domingues, William B.
author_facet Domingues, William B.
Silveira, Tony L. R.
Nunes, Leandro S.
Blodorn, Eduardo B.
Schneider, Augusto
Corcine, Carine D.
Varela Junior, Antônio S.
Acosta, Izani B.
Kütter, Mateus T.
Greif, Gonzalo
Robello, Carlos
Pinhal, Danillo [UNESP]
Marins, Luís F.
Campos, Vinicius F.
author_role author
author2 Silveira, Tony L. R.
Nunes, Leandro S.
Blodorn, Eduardo B.
Schneider, Augusto
Corcine, Carine D.
Varela Junior, Antônio S.
Acosta, Izani B.
Kütter, Mateus T.
Greif, Gonzalo
Robello, Carlos
Pinhal, Danillo [UNESP]
Marins, Luís F.
Campos, Vinicius F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Pelotas
Universidade Federal do Rio Grande
Institut Pasteur
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Domingues, William B.
Silveira, Tony L. R.
Nunes, Leandro S.
Blodorn, Eduardo B.
Schneider, Augusto
Corcine, Carine D.
Varela Junior, Antônio S.
Acosta, Izani B.
Kütter, Mateus T.
Greif, Gonzalo
Robello, Carlos
Pinhal, Danillo [UNESP]
Marins, Luís F.
Campos, Vinicius F.
dc.subject.por.fl_str_mv Danio rerio
epigenetic
miRNA-seq
non-coding RNAs
sperm motility
transgenic fish
topic Danio rerio
epigenetic
miRNA-seq
non-coding RNAs
sperm motility
transgenic fish
description Overexpression of growth hormone (GH) in gh-transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. It is hypothesized that microRNAs are directly involved in the regulation of fertility potential during spermatogenesis. The primary aim of our study was to verify whether gh overexpression disturbs the sperm miRNA profile and influences the sperm quality in transgenic zebrafish. We report a significant increase in body weight of gh-transgenic males along with associated reduced sperm motility and other kinetic parameters in comparison to the non-transgenic group. MicroRNA transcriptome sequencing of gh-transgenic zebrafish sperms revealed expressions of 186 miRNAs, among which six miRNA were up-regulated (miR-146b, miR-200a-5p, miR-146a, miR-726, miR-184, and miR-738) and sixteen were down-regulated (miR-19d-3p, miR-126a-5p, miR-126b-5p, miR-22a-5p, miR-16c-5p, miR-20a-5p, miR-126b-3p, miR-107a-3p, miR-93, miR-2189, miR-202–5p, miR-221–3p, miR-125a, miR-125b-5p, miR-126a-3p, and miR-30c-5p) in comparison to non-transgenic zebrafish. Some of the dysregulated miRNAs were previously reported to be related to abnormalities in sperm quality and reduced reproduction ability in other species. In this study, an average of 134 differentially expressed miRNAs-targeted genes were predicted using the in silico approach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that the genes of affected pathways were primarily related to spermatogenesis, sperm motility, and cell apoptosis. Our results suggested that excess GH caused a detrimental effect on sperm microRNAome, consequently reducing the sperm quality and reproductive potential of zebrafish males.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-08
2022-04-29T08:33:13Z
2022-04-29T08:33:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fgene.2021.704778
Frontiers in Genetics, v. 12.
1664-8021
http://hdl.handle.net/11449/229560
10.3389/fgene.2021.704778
2-s2.0-85115357322
url http://dx.doi.org/10.3389/fgene.2021.704778
http://hdl.handle.net/11449/229560
identifier_str_mv Frontiers in Genetics, v. 12.
1664-8021
10.3389/fgene.2021.704778
2-s2.0-85115357322
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Genetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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