Avaliação de parâmetros de cicatrização no cólon de ratos diabéticos sem agressão cirúrgica

• Autor(a) principal: Ortolan, Erika Veruska Paiva [UNESP]
• Data de Publicação: 2004
• Outros Autores: Spadella, César Tadeu [UNESP], Machado, José Lúcio Martins [UNESP], Kobayasi, Shoiti [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502004000300011; http://hdl.handle.net/11449/212305
• topic: Experimental diabetes mellitus; Healing; Colon; Diabetes mellitus; Experimental; Cicatrização; Cólon

PURPOSE: There are few studies using healing parameters in diabetic animals before the wound, and no studies in the colon. The aim of this study was to verify if alloxan diabetes-induced could primarily change parameters commonly used to measure the small bowel healing in surgical wounded rats, although using animals without any surgical procedure. METHODS: 180 rats were assigned to in two groups, of non-diabetic control animals, and diabetic animals. The animals were considered diabetics if blood glucose level>200mg/dl and urinary glucose level>3000mg/dl. After 3 months, both groups were evaluated in 6 moments of sacrifice, when were analyzed diabetes (blood and urinary glucose levels and plasmatic insulin) and healing in the colon (breaking strength, hydroxyproline, total tissue protein and the ratio OHP/TTP). RESULTS: Alloxan 2% caused 42,3% of mortality and 72,4% of severe diabetes. All animals in the diabetic group (G2) presented with blood glucose>300mg/dl and urinary glucose>3000mg/dl and significant decrease in plasmatic insulin. Neither the breaking strength, nor the biochemical dosages (HOP, TTP) showed up any meaningful statistical variation between groups. CONCLUSION: The findings of our study suggest that diabetes by itself is not able to change healing parameters, before the surgical injury in the colon of rats.