Genes de resistência aos β-lactâmicos, macrolídeos e tetraciclinas em amostras de caprinos com periodontite e saudáveis

• Autor(a) principal: Dias, Nathália [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155302; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-02/000881517.pdf
• topic: Periodontite; Ruminante; Bactérias anaeróbicas; Resistência microbiana a medicamentos; Tetraciclinas; beta-Lactamas; Periodontitis

Periodontitis is an infectious disease potentially debilitating in goats. It is associated with different anaerobic microbial groups after loss of the ecological balance in the biofilm possibly by contact with subinibitory concentrations of antimicrobials produced by soil microorganisms, which enhance the probability of the presence of microorganisms resistant to these drugs in the oral microbiota of affected animals. The aim of the study was to evaluate the occurrence of antimicrobial resistance markers in the subgingival biofilm from goats with periodontitis and from periodontal healthy animals. Clinical specimens were collected from the biofilm of 36 goats with periodontitis and 20 periodontally healthy animals. After extraction of microbial DNA, it was evaluated the presence of genes tetM, tetO, tetQ, blaTEM, blaSHV, blaCTX-M, ermA and ermF by polymerase chain reaction (PCR). The variables with three or more categories were submitted to the Chi-Square test and the possible associations between the studied parameters were determined using the Spearman correlation test. The bla genes and ermA were not detected in clinical specimens, while genes ermF, tetM, tetQ were observed in 36.4%, 22.7% and 36.4% of the animals with periodontitis, respectively, but not in specimans from healthy animals, which was statistically significant. The gene tetO was detected in 36.4% of the specimens from animals with periodontitis and from 9.1% healthy specimens. The results allow the conclusion that the biofilm from goats with periodontitis has higher frequency detection of certain antimicrobial resistance markers