Análise global da diversidade de microorganismo importantes para a fermentação nas usinas de álcool: uma abordagem utilizando metagenômica

• Autor(a) principal: Lima, Larissa Barbosa de [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/124182; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2015-05-11/000829013.pdf
• topic: Álcool - Combustível - Indústria; Álcool como combustível - Contaminação; Lactobacilo; Metagenomica; Fermentação; Etanol; Contaminação; Alcohol as fuel

Brazil is the second world's largest producer of ethanol and, to keep this production, there is a huge concern by this industry regarding bacterial contamination. Due to the non-sterile process conditions, bacteria present in the raw material that fuels the production of ethanol are common contaminants. To combat them, factories uses randomly antimicrobial, a practice that increases the price of alcohol in addition to generating resistant bacteria and leave waste on the environment. This said, the identification of contaminants in different industrial environments is necessary, as well as the search for new technologies for the control of contaminants from alcoholic fermentation. In this project, it will be shown that the molecular technique of using metagenomic 16S, applied to samples of content of the tanks of the 2013 harvest, corroborates earlier studies and shows it as a rapid and efficient method for identifying contaminating microorganisms. Our results obtained using next generation sequencing in high-throughput platform confirm the predominance of Lactobacillus spp in fermentation, and the presence of other contaminants not previously described as Rubrobacter xylanophilus, Cloacibacterium normanense and Diaphorobacter nitroreducens, which enables the development of personalized treatment