Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00284-020-02166-1 http://hdl.handle.net/11449/199299 |
Resumo: | The dry cell weight (DCW) measurement is one of the preferred methods to determine the growth of filamentous fungi. However, this technique is not applicable to insoluble culture media, besides being possibly influenced by the presence of extracellular biomass. The standard plate counting (SPC) is a reference method for detecting viable cells; however, it is referred as imprecise. In this study, we did a comprehensive analysis of the errors associated to each procedure and also determined the growth kinetics of Fusarium oxysporum in soluble (DCW and SPC) and insoluble (SPC) culture media. Finally, we used the production of bikaverin in airlift bioreactor containing insoluble medium as a case study to estimate red pigment production and to monitor biomass growth via SPC. We concluded that SPC can be used to give reliable fungal growth kinetics in media with insoluble matter, yielding errors equivalent to DCW depending on the number of replicates done for serial dilutions and plate counting. |
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spelling |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a ModelThe dry cell weight (DCW) measurement is one of the preferred methods to determine the growth of filamentous fungi. However, this technique is not applicable to insoluble culture media, besides being possibly influenced by the presence of extracellular biomass. The standard plate counting (SPC) is a reference method for detecting viable cells; however, it is referred as imprecise. In this study, we did a comprehensive analysis of the errors associated to each procedure and also determined the growth kinetics of Fusarium oxysporum in soluble (DCW and SPC) and insoluble (SPC) culture media. Finally, we used the production of bikaverin in airlift bioreactor containing insoluble medium as a case study to estimate red pigment production and to monitor biomass growth via SPC. We concluded that SPC can be used to give reliable fungal growth kinetics in media with insoluble matter, yielding errors equivalent to DCW depending on the number of replicates done for serial dilutions and plate counting.Faculdade de Engenharia de Alimentos Department of Food Science University of Campinas, Rua Monteiro Lobato, 80Federal University of São João Del-Rei. Ouro Branco, Campus Alto ParaopebaDepartment of Analytical Chemistry UNESP Universidade Estadual PaulistaDepartment of Bioprocess and Biotechnology UNESP Universidade Estadual PaulistaChemical Engineering Department Federal University of São Carlos UFSCarDepartment of Analytical Chemistry UNESP Universidade Estadual PaulistaDepartment of Bioprocess and Biotechnology UNESP Universidade Estadual PaulistaUniversidade Estadual de Campinas (UNICAMP)Federal University of São João Del-Rei. Ouro BrancoUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)dos Santos, Marcela Colomboda Silva, Wesley Santiagoda Silva, Bianca Ferreira [UNESP]Cerri, Marcel Otavio [UNESP]Ribeiro, Marcelo Perencin de ArrudaBicas, Juliano Lemos2020-12-12T01:36:05Z2020-12-12T01:36:05Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00284-020-02166-1Current Microbiology.1432-09910343-8651http://hdl.handle.net/11449/19929910.1007/s00284-020-02166-12-s2.0-85089863340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T07:00:31Zoai:repositorio.unesp.br:11449/199299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T07:00:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
title |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
spellingShingle |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model dos Santos, Marcela Colombo |
title_short |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
title_full |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
title_fullStr |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
title_full_unstemmed |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
title_sort |
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model |
author |
dos Santos, Marcela Colombo |
author_facet |
dos Santos, Marcela Colombo da Silva, Wesley Santiago da Silva, Bianca Ferreira [UNESP] Cerri, Marcel Otavio [UNESP] Ribeiro, Marcelo Perencin de Arruda Bicas, Juliano Lemos |
author_role |
author |
author2 |
da Silva, Wesley Santiago da Silva, Bianca Ferreira [UNESP] Cerri, Marcel Otavio [UNESP] Ribeiro, Marcelo Perencin de Arruda Bicas, Juliano Lemos |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Federal University of São João Del-Rei. Ouro Branco Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
dos Santos, Marcela Colombo da Silva, Wesley Santiago da Silva, Bianca Ferreira [UNESP] Cerri, Marcel Otavio [UNESP] Ribeiro, Marcelo Perencin de Arruda Bicas, Juliano Lemos |
description |
The dry cell weight (DCW) measurement is one of the preferred methods to determine the growth of filamentous fungi. However, this technique is not applicable to insoluble culture media, besides being possibly influenced by the presence of extracellular biomass. The standard plate counting (SPC) is a reference method for detecting viable cells; however, it is referred as imprecise. In this study, we did a comprehensive analysis of the errors associated to each procedure and also determined the growth kinetics of Fusarium oxysporum in soluble (DCW and SPC) and insoluble (SPC) culture media. Finally, we used the production of bikaverin in airlift bioreactor containing insoluble medium as a case study to estimate red pigment production and to monitor biomass growth via SPC. We concluded that SPC can be used to give reliable fungal growth kinetics in media with insoluble matter, yielding errors equivalent to DCW depending on the number of replicates done for serial dilutions and plate counting. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:36:05Z 2020-12-12T01:36:05Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00284-020-02166-1 Current Microbiology. 1432-0991 0343-8651 http://hdl.handle.net/11449/199299 10.1007/s00284-020-02166-1 2-s2.0-85089863340 |
url |
http://dx.doi.org/10.1007/s00284-020-02166-1 http://hdl.handle.net/11449/199299 |
identifier_str_mv |
Current Microbiology. 1432-0991 0343-8651 10.1007/s00284-020-02166-1 2-s2.0-85089863340 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650036572094464 |