How can micelle systems be rebuilt by a heating process?
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2147/IJN.S25761 http://hdl.handle.net/11449/7920 |
Resumo: | The aim of this work was to evaluate how an aqueous micellar system - containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg.L-1, 5 mg.L-1, 0.5 mg.L-1, and 0.05 mg.L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg.L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. |
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How can micelle systems be rebuilt by a heating process?micellesnanotechnologypre-heated amphotericin Bsuper-aggregatesThe aim of this work was to evaluate how an aqueous micellar system - containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg.L-1, 5 mg.L-1, 0.5 mg.L-1, and 0.05 mg.L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg.L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fed Univ Rio Grande Norte UFRN, Dispersed Syst Lab, BR-59094450 Natal, RN, BrazilUniv Fed Rio Grande do Norte, Dept Pharm, Natal, RN, BrazilUniv Fed Rio Grande do Norte, Dept Expt Surg, Natal, RN, BrazilColl Pharmaceut Sci UNESP, Dept Drugs & Med, São Paulo, BrazilColl Pharmaceut Sci UNESP, Dept Drugs & Med, São Paulo, BrazilCNPq: 301979/04-9CNPq: 473882/04-3CNPq: 47836/01-7-NVDove Medical Press LtdUniversidade Federal do Rio Grande do Norte (UFRN)Universidade Estadual Paulista (Unesp)da Silva-Filho, Miguel AdelinoVieira da Silva Siqueira, Scheyla DanielaFreire, Larissa Bandeirade Araujo, Ivonete BatistaGyselle de Holanda e Silva, KattyaMedeiros, Aldo da CunhaAraujo-Filho, IramiOliveira, Anselmo Gomes de [UNESP]Tabosa do Egito, Eryvaldo Socrates2014-05-20T13:25:02Z2014-05-20T13:25:02Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-150application/pdfhttp://dx.doi.org/10.2147/IJN.S25761International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 7, p. 141-150, 2012.1178-2013http://hdl.handle.net/11449/792010.2147/IJN.S25761WOS:000302700700001WOS000302700700001.pdf9114495952533044Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Nanomedicine4.3701,225info:eu-repo/semantics/openAccess2024-06-24T13:45:30Zoai:repositorio.unesp.br:11449/7920Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:02:54.207723Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
How can micelle systems be rebuilt by a heating process? |
title |
How can micelle systems be rebuilt by a heating process? |
spellingShingle |
How can micelle systems be rebuilt by a heating process? da Silva-Filho, Miguel Adelino micelles nanotechnology pre-heated amphotericin B super-aggregates |
title_short |
How can micelle systems be rebuilt by a heating process? |
title_full |
How can micelle systems be rebuilt by a heating process? |
title_fullStr |
How can micelle systems be rebuilt by a heating process? |
title_full_unstemmed |
How can micelle systems be rebuilt by a heating process? |
title_sort |
How can micelle systems be rebuilt by a heating process? |
author |
da Silva-Filho, Miguel Adelino |
author_facet |
da Silva-Filho, Miguel Adelino Vieira da Silva Siqueira, Scheyla Daniela Freire, Larissa Bandeira de Araujo, Ivonete Batista Gyselle de Holanda e Silva, Kattya Medeiros, Aldo da Cunha Araujo-Filho, Irami Oliveira, Anselmo Gomes de [UNESP] Tabosa do Egito, Eryvaldo Socrates |
author_role |
author |
author2 |
Vieira da Silva Siqueira, Scheyla Daniela Freire, Larissa Bandeira de Araujo, Ivonete Batista Gyselle de Holanda e Silva, Kattya Medeiros, Aldo da Cunha Araujo-Filho, Irami Oliveira, Anselmo Gomes de [UNESP] Tabosa do Egito, Eryvaldo Socrates |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte (UFRN) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Silva-Filho, Miguel Adelino Vieira da Silva Siqueira, Scheyla Daniela Freire, Larissa Bandeira de Araujo, Ivonete Batista Gyselle de Holanda e Silva, Kattya Medeiros, Aldo da Cunha Araujo-Filho, Irami Oliveira, Anselmo Gomes de [UNESP] Tabosa do Egito, Eryvaldo Socrates |
dc.subject.por.fl_str_mv |
micelles nanotechnology pre-heated amphotericin B super-aggregates |
topic |
micelles nanotechnology pre-heated amphotericin B super-aggregates |
description |
The aim of this work was to evaluate how an aqueous micellar system - containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg.L-1, 5 mg.L-1, 0.5 mg.L-1, and 0.05 mg.L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg.L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:25:02Z 2014-05-20T13:25:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2147/IJN.S25761 International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 7, p. 141-150, 2012. 1178-2013 http://hdl.handle.net/11449/7920 10.2147/IJN.S25761 WOS:000302700700001 WOS000302700700001.pdf 9114495952533044 |
url |
http://dx.doi.org/10.2147/IJN.S25761 http://hdl.handle.net/11449/7920 |
identifier_str_mv |
International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 7, p. 141-150, 2012. 1178-2013 10.2147/IJN.S25761 WOS:000302700700001 WOS000302700700001.pdf 9114495952533044 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Nanomedicine 4.370 1,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
141-150 application/pdf |
dc.publisher.none.fl_str_mv |
Dove Medical Press Ltd |
publisher.none.fl_str_mv |
Dove Medical Press Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128744911536128 |