Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0048 http://hdl.handle.net/11449/177019 |
Resumo: | Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials. |
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Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineageSeveral calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.Universidade Estadual Paulista - UNESP, Araraquara School of Dentistry, Department of Restorative Dentistry, Araraquara, SP, BrazilUniversidade Estadual Paulista - UNESP, School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, SP, BrazilUniversidade Estadual Paulista - UNESP, Araraquara School of Dentistry, Department of Diagnosis and Surgery, Araraquara, SP, BrazilUniversidade Estadual Paulista (Unesp)Gomes-Cornélio, Ana LíviaRodrigues, Elisandra MárciaMestieri, Leticia BoldrinFalcoski, Thaís de Oliveira Rodrigues SanzovoSoares, Christiane PiennaGuerreiro-Tanomaru, Juliane MariaRossa, CarlosTanomaru, Mário2018-12-11T17:23:31Z2018-12-11T17:23:31Z2016-05-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0048Brazilian oral research, v. 30, n. 1, 2016.1807-3107http://hdl.handle.net/11449/17701910.1590/1807-3107BOR-2016.vol30.0048S1806-832420160001002472-s2.0-85021854765S1806-83242016000100247.pdf17680252903736690000-0003-1740-7360Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian oral researchinfo:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/177019Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:42.605239Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
title |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
spellingShingle |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage Gomes-Cornélio, Ana Lívia |
title_short |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
title_full |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
title_fullStr |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
title_full_unstemmed |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
title_sort |
Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
author |
Gomes-Cornélio, Ana Lívia |
author_facet |
Gomes-Cornélio, Ana Lívia Rodrigues, Elisandra Márcia Mestieri, Leticia Boldrin Falcoski, Thaís de Oliveira Rodrigues Sanzovo Soares, Christiane Pienna Guerreiro-Tanomaru, Juliane Maria Rossa, Carlos Tanomaru, Mário |
author_role |
author |
author2 |
Rodrigues, Elisandra Márcia Mestieri, Leticia Boldrin Falcoski, Thaís de Oliveira Rodrigues Sanzovo Soares, Christiane Pienna Guerreiro-Tanomaru, Juliane Maria Rossa, Carlos Tanomaru, Mário |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes-Cornélio, Ana Lívia Rodrigues, Elisandra Márcia Mestieri, Leticia Boldrin Falcoski, Thaís de Oliveira Rodrigues Sanzovo Soares, Christiane Pienna Guerreiro-Tanomaru, Juliane Maria Rossa, Carlos Tanomaru, Mário |
description |
Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-20 2018-12-11T17:23:31Z 2018-12-11T17:23:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0048 Brazilian oral research, v. 30, n. 1, 2016. 1807-3107 http://hdl.handle.net/11449/177019 10.1590/1807-3107BOR-2016.vol30.0048 S1806-83242016000100247 2-s2.0-85021854765 S1806-83242016000100247.pdf 1768025290373669 0000-0003-1740-7360 |
url |
http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0048 http://hdl.handle.net/11449/177019 |
identifier_str_mv |
Brazilian oral research, v. 30, n. 1, 2016. 1807-3107 10.1590/1807-3107BOR-2016.vol30.0048 S1806-83242016000100247 2-s2.0-85021854765 S1806-83242016000100247.pdf 1768025290373669 0000-0003-1740-7360 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian oral research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128448746487808 |