Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.gene.2020.144477 http://hdl.handle.net/11449/196753 |
Resumo: | The wide variation found in the size of eukaryotic genomes is largely related to the accumulation of repetitive sequences. Studies show that these sequences can go through an evolutionary process (molecular co-optation) and acquire new genomic functions. Cytogenetic studies reveal a wide karyotypic variation between chelonians (order Testudines) (2n = 26-68), attributed mainly to the number of microchromosomes. The study of repetitive DNAs has the potential to provide data on the dynamics of these sequences, and how they influence the organization of the genome. Here, we reveal the first in situ mapping data of 45S rDNA, histone H3 genes, and telomeric sequences, for a species of the genus Rhinoclemmys, R. punctularia. The karyotype described here for R. punctularia is different from previous reports for the diploid complement of this species, with differences probably attributable to centric fissions and pericentric inversions or centromere repositioning. The 45S rDNA are on a single chromosome pair (like in other turtles), telomeric sequences are in terminal position on all the chromosomes, and histone H3 is dispersed in low copy number, with clusters in pericentromeric regions of three chromosome pairs. We report on the presence of a Gypsy retrotransposon insert located within H3 histone of R. punctularia, and the H3 region sequenced contained the open reading frame of the histone sequence. Comparative modeling revealed a functional pattern for the protein, thus suggesting that the Gypsy element might have been recruited for new functions in the genome of this species. |
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Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genomeHistone genesRetrotransposon LTR45S rDNAKaryotypic reorganizationTurtleThe wide variation found in the size of eukaryotic genomes is largely related to the accumulation of repetitive sequences. Studies show that these sequences can go through an evolutionary process (molecular co-optation) and acquire new genomic functions. Cytogenetic studies reveal a wide karyotypic variation between chelonians (order Testudines) (2n = 26-68), attributed mainly to the number of microchromosomes. The study of repetitive DNAs has the potential to provide data on the dynamics of these sequences, and how they influence the organization of the genome. Here, we reveal the first in situ mapping data of 45S rDNA, histone H3 genes, and telomeric sequences, for a species of the genus Rhinoclemmys, R. punctularia. The karyotype described here for R. punctularia is different from previous reports for the diploid complement of this species, with differences probably attributable to centric fissions and pericentric inversions or centromere repositioning. The 45S rDNA are on a single chromosome pair (like in other turtles), telomeric sequences are in terminal position on all the chromosomes, and histone H3 is dispersed in low copy number, with clusters in pericentromeric regions of three chromosome pairs. We report on the presence of a Gypsy retrotransposon insert located within H3 histone of R. punctularia, and the H3 region sequenced contained the open reading frame of the histone sequence. Comparative modeling revealed a functional pattern for the protein, thus suggesting that the Gypsy element might have been recruited for new functions in the genome of this species.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Bank for Economic and Social Development - BNDESCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Para, Ctr Estudos Avancados Biodiversidade, Inst Ciencias Biol, Lab Citogenet, Belem, Para, BrazilUniv Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Ponta Grossa, Parana, BrazilUniv Fed Rural Amazonia, Campus Capanema, Capanema, Parana, BrazilUniv Estadual Paulista, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Morfol, Botucatu, SP, BrazilCNPq: 475013/2012-3National Bank for Economic and Social Development - BNDES: 2.318.697.0001CNPq: 47/2012CNPq: 305880/2017-9CNPq: 305876/2017-1Elsevier B.V.Univ Fed ParaUniversidade Estadual de Ponta Grossa (UEPG)Univ Fed Rural AmazoniaUniversidade Estadual Paulista (Unesp)Cavalcante, Manoella GemaqueSouza, Luciano FariasVicari, Marcelo RicardoMatos de Bastos, Carlos EduardoSousa, Jaime Viana deNagamachi, Cleusa YoshikoPieczarka, Julio CesarMartins, Cesar [UNESP]Rodrigues Noronha, Renata Coelho2020-12-10T19:55:10Z2020-12-10T19:55:10Z2020-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.gene.2020.144477Gene. Amsterdam: Elsevier, v. 738, 7 p., 2020.0378-1119http://hdl.handle.net/11449/19675310.1016/j.gene.2020.144477WOS:00052362920001388588006994253520000-0003-3534-974XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGeneinfo:eu-repo/semantics/openAccess2021-10-23T22:14:28Zoai:repositorio.unesp.br:11449/196753Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:47.110332Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
title |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
spellingShingle |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome Cavalcante, Manoella Gemaque Histone genes Retrotransposon LTR 45S rDNA Karyotypic reorganization Turtle |
title_short |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
title_full |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
title_fullStr |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
title_full_unstemmed |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
title_sort |
Molecular cytogenetics characterization of Rhinoclemmys punctularia (Testudines, Geoemydidae) and description of a Gypsy-H3 association in its genome |
author |
Cavalcante, Manoella Gemaque |
author_facet |
Cavalcante, Manoella Gemaque Souza, Luciano Farias Vicari, Marcelo Ricardo Matos de Bastos, Carlos Eduardo Sousa, Jaime Viana de Nagamachi, Cleusa Yoshiko Pieczarka, Julio Cesar Martins, Cesar [UNESP] Rodrigues Noronha, Renata Coelho |
author_role |
author |
author2 |
Souza, Luciano Farias Vicari, Marcelo Ricardo Matos de Bastos, Carlos Eduardo Sousa, Jaime Viana de Nagamachi, Cleusa Yoshiko Pieczarka, Julio Cesar Martins, Cesar [UNESP] Rodrigues Noronha, Renata Coelho |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Para Universidade Estadual de Ponta Grossa (UEPG) Univ Fed Rural Amazonia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cavalcante, Manoella Gemaque Souza, Luciano Farias Vicari, Marcelo Ricardo Matos de Bastos, Carlos Eduardo Sousa, Jaime Viana de Nagamachi, Cleusa Yoshiko Pieczarka, Julio Cesar Martins, Cesar [UNESP] Rodrigues Noronha, Renata Coelho |
dc.subject.por.fl_str_mv |
Histone genes Retrotransposon LTR 45S rDNA Karyotypic reorganization Turtle |
topic |
Histone genes Retrotransposon LTR 45S rDNA Karyotypic reorganization Turtle |
description |
The wide variation found in the size of eukaryotic genomes is largely related to the accumulation of repetitive sequences. Studies show that these sequences can go through an evolutionary process (molecular co-optation) and acquire new genomic functions. Cytogenetic studies reveal a wide karyotypic variation between chelonians (order Testudines) (2n = 26-68), attributed mainly to the number of microchromosomes. The study of repetitive DNAs has the potential to provide data on the dynamics of these sequences, and how they influence the organization of the genome. Here, we reveal the first in situ mapping data of 45S rDNA, histone H3 genes, and telomeric sequences, for a species of the genus Rhinoclemmys, R. punctularia. The karyotype described here for R. punctularia is different from previous reports for the diploid complement of this species, with differences probably attributable to centric fissions and pericentric inversions or centromere repositioning. The 45S rDNA are on a single chromosome pair (like in other turtles), telomeric sequences are in terminal position on all the chromosomes, and histone H3 is dispersed in low copy number, with clusters in pericentromeric regions of three chromosome pairs. We report on the presence of a Gypsy retrotransposon insert located within H3 histone of R. punctularia, and the H3 region sequenced contained the open reading frame of the histone sequence. Comparative modeling revealed a functional pattern for the protein, thus suggesting that the Gypsy element might have been recruited for new functions in the genome of this species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:55:10Z 2020-12-10T19:55:10Z 2020-05-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.gene.2020.144477 Gene. Amsterdam: Elsevier, v. 738, 7 p., 2020. 0378-1119 http://hdl.handle.net/11449/196753 10.1016/j.gene.2020.144477 WOS:000523629200013 8858800699425352 0000-0003-3534-974X |
url |
http://dx.doi.org/10.1016/j.gene.2020.144477 http://hdl.handle.net/11449/196753 |
identifier_str_mv |
Gene. Amsterdam: Elsevier, v. 738, 7 p., 2020. 0378-1119 10.1016/j.gene.2020.144477 WOS:000523629200013 8858800699425352 0000-0003-3534-974X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129296924934144 |