Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2020/3695683 http://hdl.handle.net/11449/201557 |
Resumo: | Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage. |
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Repositório Institucional da UNESP |
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spelling |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's SalivaOxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage.Graduate Program in Dental Science São Paulo State University (UNESP) School of DentistryDepartment of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of DentistryMulticenter Graduate Program in Physiological Sciences São Paulo State University (UNESP) School of DentistryDepartment of Basic Sciences São Paulo State University (UNESP) School of DentistryGraduate Program in Dental Science São Paulo State University (UNESP) School of DentistryDepartment of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of DentistryMulticenter Graduate Program in Physiological Sciences São Paulo State University (UNESP) School of DentistryDepartment of Basic Sciences São Paulo State University (UNESP) School of DentistryUniversidade Estadual Paulista (Unesp)Araujo, Heitor Ceolin [UNESP]Nakamune, Ana Cláudia Melo Stevanato [UNESP]Garcia, Wilson Galhego [UNESP]Pessan, Juliano Pelim [UNESP]Antoniali, Cristina [UNESP]2020-12-12T02:35:43Z2020-12-12T02:35:43Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2020/3695683Oxidative Medicine and Cellular Longevity, v. 2020.1942-09941942-0900http://hdl.handle.net/11449/20155710.1155/2020/36956832-s2.0-85079411070Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOxidative Medicine and Cellular Longevityinfo:eu-repo/semantics/openAccess2023-02-06T13:42:30Zoai:repositorio.unesp.br:11449/201557Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:54.320683Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
title |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
spellingShingle |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva Araujo, Heitor Ceolin [UNESP] |
title_short |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
title_full |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
title_fullStr |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
title_full_unstemmed |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
title_sort |
Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children's Saliva |
author |
Araujo, Heitor Ceolin [UNESP] |
author_facet |
Araujo, Heitor Ceolin [UNESP] Nakamune, Ana Cláudia Melo Stevanato [UNESP] Garcia, Wilson Galhego [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
author_role |
author |
author2 |
Nakamune, Ana Cláudia Melo Stevanato [UNESP] Garcia, Wilson Galhego [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Araujo, Heitor Ceolin [UNESP] Nakamune, Ana Cláudia Melo Stevanato [UNESP] Garcia, Wilson Galhego [UNESP] Pessan, Juliano Pelim [UNESP] Antoniali, Cristina [UNESP] |
description |
Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:43Z 2020-12-12T02:35:43Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2020/3695683 Oxidative Medicine and Cellular Longevity, v. 2020. 1942-0994 1942-0900 http://hdl.handle.net/11449/201557 10.1155/2020/3695683 2-s2.0-85079411070 |
url |
http://dx.doi.org/10.1155/2020/3695683 http://hdl.handle.net/11449/201557 |
identifier_str_mv |
Oxidative Medicine and Cellular Longevity, v. 2020. 1942-0994 1942-0900 10.1155/2020/3695683 2-s2.0-85079411070 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oxidative Medicine and Cellular Longevity |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129193482911744 |