Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/AN17157 http://hdl.handle.net/11449/184453 |
Resumo: | Repeatability coefficients (r) for tick resistance and Babesia bovis infection levels and the correlation () between these traits were estimated in beef heifers, using artificial infestations and short intervals between measurements. Forty heifers, including 20 Bos taurus taurus (Caracu) and 20 Bos taurus indicus (Nelore) animals, were submitted to three artificial infestations with Rhipicephalus microplus larvae at intervals of 14 days. The number of standard female was counted from the 19th through the 23rd day after each infestation, considering only the left side of each animal. Blood samples were collected on Days 0, 6, 20, 34 and 48 after the first infestation. The number of copies of B. bovis DNA (CN) was estimated from blood samples through the quantitative PCR technique to evaluate the level of infection in the animals. Total tick count (TTC), the percentage of return (PRij) and CN were analysed using the MIXED procedure of the SAS program. The r of the variables were estimated by intraclass correlation between measures of the same animal, with the variance component of the animal being divided by the phenotypic variance (residual + animal), using models with a CS structure matrix. Additionally, among variables were estimated using the CORR procedure. The following results were obtained for Caracu and Nelore animals, respectively: 1.83 +/- 0.37 and 0.63 +/- 0.40 for TTC, 1.10 +/- 0.23 and 0.47 +/- 0.23 for PRij, and 2.29 +/- 0.64 and 2.32 +/- 0.58 for CN. The r was moderate for TTC (0.62) and PRij (0.53) and low for CN (0.10). The between TTC and CN obtained in the same measurement day was not significant in either breed (P > 0.05; -0.07 for Caracu and 0.19 for Nelore). Results showed both breeds were able to develop resistance against B. bovis; however, Nelore exhibited higher tick resistance. The r obtained for CN and the weak association with tick resistance indicate that it is not possible to recommend the use of CN as a trait to predict tick resistance in these two breeds. |
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Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazilartificial infestationscattleqPCRrepeatabilityRepeatability coefficients (r) for tick resistance and Babesia bovis infection levels and the correlation () between these traits were estimated in beef heifers, using artificial infestations and short intervals between measurements. Forty heifers, including 20 Bos taurus taurus (Caracu) and 20 Bos taurus indicus (Nelore) animals, were submitted to three artificial infestations with Rhipicephalus microplus larvae at intervals of 14 days. The number of standard female was counted from the 19th through the 23rd day after each infestation, considering only the left side of each animal. Blood samples were collected on Days 0, 6, 20, 34 and 48 after the first infestation. The number of copies of B. bovis DNA (CN) was estimated from blood samples through the quantitative PCR technique to evaluate the level of infection in the animals. Total tick count (TTC), the percentage of return (PRij) and CN were analysed using the MIXED procedure of the SAS program. The r of the variables were estimated by intraclass correlation between measures of the same animal, with the variance component of the animal being divided by the phenotypic variance (residual + animal), using models with a CS structure matrix. Additionally, among variables were estimated using the CORR procedure. The following results were obtained for Caracu and Nelore animals, respectively: 1.83 +/- 0.37 and 0.63 +/- 0.40 for TTC, 1.10 +/- 0.23 and 0.47 +/- 0.23 for PRij, and 2.29 +/- 0.64 and 2.32 +/- 0.58 for CN. The r was moderate for TTC (0.62) and PRij (0.53) and low for CN (0.10). The between TTC and CN obtained in the same measurement day was not significant in either breed (P > 0.05; -0.07 for Caracu and 0.19 for Nelore). Results showed both breeds were able to develop resistance against B. bovis; however, Nelore exhibited higher tick resistance. The r obtained for CN and the weak association with tick resistance indicate that it is not possible to recommend the use of CN as a trait to predict tick resistance in these two breeds.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilEmpresa Brasileira Pesquisa Agr, Sao Carlos, SP, BrazilInst Zootecnia, Sertaozinho, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilCsiro PublishingUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Inst ZootecniaMaiorano, A. M. [UNESP]Giglioti, R. [UNESP]Oliveira, M. C. S.Oliveira, H. N. [UNESP]Cyrillo, J. N. S. G.Mercadante, M. E. Z.Silva, J. A. I. I. V. [UNESP]2019-10-04T12:13:42Z2019-10-04T12:13:42Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article938-944http://dx.doi.org/10.1071/AN17157Animal Production Science. Clayton: Csiro Publishing, v. 59, n. 5, p. 938-944, 2019.1836-0939http://hdl.handle.net/11449/18445310.1071/AN17157WOS:000465239400015Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Production Scienceinfo:eu-repo/semantics/openAccess2021-10-23T17:52:05Zoai:repositorio.unesp.br:11449/184453Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:00:48.570947Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
title |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
spellingShingle |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil Maiorano, A. M. [UNESP] artificial infestations cattle qPCR repeatability |
title_short |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
title_full |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
title_fullStr |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
title_full_unstemmed |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
title_sort |
Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil |
author |
Maiorano, A. M. [UNESP] |
author_facet |
Maiorano, A. M. [UNESP] Giglioti, R. [UNESP] Oliveira, M. C. S. Oliveira, H. N. [UNESP] Cyrillo, J. N. S. G. Mercadante, M. E. Z. Silva, J. A. I. I. V. [UNESP] |
author_role |
author |
author2 |
Giglioti, R. [UNESP] Oliveira, M. C. S. Oliveira, H. N. [UNESP] Cyrillo, J. N. S. G. Mercadante, M. E. Z. Silva, J. A. I. I. V. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Inst Zootecnia |
dc.contributor.author.fl_str_mv |
Maiorano, A. M. [UNESP] Giglioti, R. [UNESP] Oliveira, M. C. S. Oliveira, H. N. [UNESP] Cyrillo, J. N. S. G. Mercadante, M. E. Z. Silva, J. A. I. I. V. [UNESP] |
dc.subject.por.fl_str_mv |
artificial infestations cattle qPCR repeatability |
topic |
artificial infestations cattle qPCR repeatability |
description |
Repeatability coefficients (r) for tick resistance and Babesia bovis infection levels and the correlation () between these traits were estimated in beef heifers, using artificial infestations and short intervals between measurements. Forty heifers, including 20 Bos taurus taurus (Caracu) and 20 Bos taurus indicus (Nelore) animals, were submitted to three artificial infestations with Rhipicephalus microplus larvae at intervals of 14 days. The number of standard female was counted from the 19th through the 23rd day after each infestation, considering only the left side of each animal. Blood samples were collected on Days 0, 6, 20, 34 and 48 after the first infestation. The number of copies of B. bovis DNA (CN) was estimated from blood samples through the quantitative PCR technique to evaluate the level of infection in the animals. Total tick count (TTC), the percentage of return (PRij) and CN were analysed using the MIXED procedure of the SAS program. The r of the variables were estimated by intraclass correlation between measures of the same animal, with the variance component of the animal being divided by the phenotypic variance (residual + animal), using models with a CS structure matrix. Additionally, among variables were estimated using the CORR procedure. The following results were obtained for Caracu and Nelore animals, respectively: 1.83 +/- 0.37 and 0.63 +/- 0.40 for TTC, 1.10 +/- 0.23 and 0.47 +/- 0.23 for PRij, and 2.29 +/- 0.64 and 2.32 +/- 0.58 for CN. The r was moderate for TTC (0.62) and PRij (0.53) and low for CN (0.10). The between TTC and CN obtained in the same measurement day was not significant in either breed (P > 0.05; -0.07 for Caracu and 0.19 for Nelore). Results showed both breeds were able to develop resistance against B. bovis; however, Nelore exhibited higher tick resistance. The r obtained for CN and the weak association with tick resistance indicate that it is not possible to recommend the use of CN as a trait to predict tick resistance in these two breeds. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:13:42Z 2019-10-04T12:13:42Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/AN17157 Animal Production Science. Clayton: Csiro Publishing, v. 59, n. 5, p. 938-944, 2019. 1836-0939 http://hdl.handle.net/11449/184453 10.1071/AN17157 WOS:000465239400015 |
url |
http://dx.doi.org/10.1071/AN17157 http://hdl.handle.net/11449/184453 |
identifier_str_mv |
Animal Production Science. Clayton: Csiro Publishing, v. 59, n. 5, p. 938-944, 2019. 1836-0939 10.1071/AN17157 WOS:000465239400015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Production Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
938-944 |
dc.publisher.none.fl_str_mv |
Csiro Publishing |
publisher.none.fl_str_mv |
Csiro Publishing |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129481670393856 |