Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma

Detalhes bibliográficos
Autor(a) principal: Oliveira, Cristiano Claudino [UNESP]
Data de Publicação: 2017
Outros Autores: Maciel-Guerra, Helena [UNESP], Kucko, Luan [UNESP], Hirama, Eric Jun [UNESP], Brilhante, Am�rico Delgado, Quevedo, Francisco Carlos, da Cunha, Isabela Werneck, Soares, Fernando Augusto, Niero-Melo, Ligia [UNESP], dos Reis, Patr�cia Pintor [UNESP], Domingues, Maria Aparecida Custodio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s13000-016-0593-0
http://hdl.handle.net/11449/174116
Resumo: Background: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing.
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spelling Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphomaCytogeneticsImmunohistochemistryIn situ hybridization fluorescenceLymphomaLymphoma, non-HodgkinBackground: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing.Department of Pathology Botucatu School of Medicine S�o Paulo State University (FMB UNESP)Botucatu School of Medicine S�o Paulo State University (FMB UNESP)AC Camargo Cancer Center Department of PathologyAmaral Carvalho Hospital Department of PathologyS�o Paulo State University (FMB UNESP) Department of Internal Medicine Botucatu School of MedicineUniversidade Estadual Paulista (UNESP) Departamento de Patologia Faculdade de Medicina de Botucatu (FMB), Distrito de Rubi�o Junior, s/nDepartment of Pathology Botucatu School of Medicine S�o Paulo State University (FMB UNESP)Botucatu School of Medicine S�o Paulo State University (FMB UNESP)S�o Paulo State University (FMB UNESP) Department of Internal Medicine Botucatu School of MedicineUniversidade Estadual Paulista (UNESP) Departamento de Patologia Faculdade de Medicina de Botucatu (FMB), Distrito de Rubi�o Junior, s/nUniversidade Estadual Paulista (Unesp)AC Camargo Cancer CenterAmaral Carvalho HospitalOliveira, Cristiano Claudino [UNESP]Maciel-Guerra, Helena [UNESP]Kucko, Luan [UNESP]Hirama, Eric Jun [UNESP]Brilhante, Am�rico DelgadoQuevedo, Francisco Carlosda Cunha, Isabela WerneckSoares, Fernando AugustoNiero-Melo, Ligia [UNESP]dos Reis, Patr�cia Pintor [UNESP]Domingues, Maria Aparecida Custodio [UNESP]2018-12-11T17:09:24Z2018-12-11T17:09:24Z2017-01-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s13000-016-0593-0Diagnostic Pathology, v. 12, n. 1, 2017.1746-1596http://hdl.handle.net/11449/17411610.1186/s13000-016-0593-02-s2.0-850101889872-s2.0-85010188987.pdf0585723113037140Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Pathology0,818info:eu-repo/semantics/openAccess2024-09-03T13:18:44Zoai:repositorio.unesp.br:11449/174116Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
title Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
spellingShingle Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
Oliveira, Cristiano Claudino [UNESP]
Cytogenetics
Immunohistochemistry
In situ hybridization fluorescence
Lymphoma
Lymphoma, non-Hodgkin
title_short Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
title_full Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
title_fullStr Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
title_full_unstemmed Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
title_sort Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
author Oliveira, Cristiano Claudino [UNESP]
author_facet Oliveira, Cristiano Claudino [UNESP]
Maciel-Guerra, Helena [UNESP]
Kucko, Luan [UNESP]
Hirama, Eric Jun [UNESP]
Brilhante, Am�rico Delgado
Quevedo, Francisco Carlos
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Niero-Melo, Ligia [UNESP]
dos Reis, Patr�cia Pintor [UNESP]
Domingues, Maria Aparecida Custodio [UNESP]
author_role author
author2 Maciel-Guerra, Helena [UNESP]
Kucko, Luan [UNESP]
Hirama, Eric Jun [UNESP]
Brilhante, Am�rico Delgado
Quevedo, Francisco Carlos
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Niero-Melo, Ligia [UNESP]
dos Reis, Patr�cia Pintor [UNESP]
Domingues, Maria Aparecida Custodio [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
AC Camargo Cancer Center
Amaral Carvalho Hospital
dc.contributor.author.fl_str_mv Oliveira, Cristiano Claudino [UNESP]
Maciel-Guerra, Helena [UNESP]
Kucko, Luan [UNESP]
Hirama, Eric Jun [UNESP]
Brilhante, Am�rico Delgado
Quevedo, Francisco Carlos
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Niero-Melo, Ligia [UNESP]
dos Reis, Patr�cia Pintor [UNESP]
Domingues, Maria Aparecida Custodio [UNESP]
dc.subject.por.fl_str_mv Cytogenetics
Immunohistochemistry
In situ hybridization fluorescence
Lymphoma
Lymphoma, non-Hodgkin
topic Cytogenetics
Immunohistochemistry
In situ hybridization fluorescence
Lymphoma
Lymphoma, non-Hodgkin
description Background: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-07
2018-12-11T17:09:24Z
2018-12-11T17:09:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s13000-016-0593-0
Diagnostic Pathology, v. 12, n. 1, 2017.
1746-1596
http://hdl.handle.net/11449/174116
10.1186/s13000-016-0593-0
2-s2.0-85010188987
2-s2.0-85010188987.pdf
0585723113037140
url http://dx.doi.org/10.1186/s13000-016-0593-0
http://hdl.handle.net/11449/174116
identifier_str_mv Diagnostic Pathology, v. 12, n. 1, 2017.
1746-1596
10.1186/s13000-016-0593-0
2-s2.0-85010188987
2-s2.0-85010188987.pdf
0585723113037140
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diagnostic Pathology
0,818
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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