Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s13000-016-0593-0 http://hdl.handle.net/11449/174116 |
Resumo: | Background: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing. |
id |
UNSP_dc55903dca7ee35d5d83a190aaf97cdf |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/174116 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphomaCytogeneticsImmunohistochemistryIn situ hybridization fluorescenceLymphomaLymphoma, non-HodgkinBackground: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing.Department of Pathology Botucatu School of Medicine S�o Paulo State University (FMB UNESP)Botucatu School of Medicine S�o Paulo State University (FMB UNESP)AC Camargo Cancer Center Department of PathologyAmaral Carvalho Hospital Department of PathologyS�o Paulo State University (FMB UNESP) Department of Internal Medicine Botucatu School of MedicineUniversidade Estadual Paulista (UNESP) Departamento de Patologia Faculdade de Medicina de Botucatu (FMB), Distrito de Rubi�o Junior, s/nDepartment of Pathology Botucatu School of Medicine S�o Paulo State University (FMB UNESP)Botucatu School of Medicine S�o Paulo State University (FMB UNESP)S�o Paulo State University (FMB UNESP) Department of Internal Medicine Botucatu School of MedicineUniversidade Estadual Paulista (UNESP) Departamento de Patologia Faculdade de Medicina de Botucatu (FMB), Distrito de Rubi�o Junior, s/nUniversidade Estadual Paulista (Unesp)AC Camargo Cancer CenterAmaral Carvalho HospitalOliveira, Cristiano Claudino [UNESP]Maciel-Guerra, Helena [UNESP]Kucko, Luan [UNESP]Hirama, Eric Jun [UNESP]Brilhante, Am�rico DelgadoQuevedo, Francisco Carlosda Cunha, Isabela WerneckSoares, Fernando AugustoNiero-Melo, Ligia [UNESP]dos Reis, Patr�cia Pintor [UNESP]Domingues, Maria Aparecida Custodio [UNESP]2018-12-11T17:09:24Z2018-12-11T17:09:24Z2017-01-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s13000-016-0593-0Diagnostic Pathology, v. 12, n. 1, 2017.1746-1596http://hdl.handle.net/11449/17411610.1186/s13000-016-0593-02-s2.0-850101889872-s2.0-85010188987.pdf0585723113037140Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Pathology0,818info:eu-repo/semantics/openAccess2024-09-03T13:18:44Zoai:repositorio.unesp.br:11449/174116Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
title |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
spellingShingle |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma Oliveira, Cristiano Claudino [UNESP] Cytogenetics Immunohistochemistry In situ hybridization fluorescence Lymphoma Lymphoma, non-Hodgkin |
title_short |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
title_full |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
title_fullStr |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
title_full_unstemmed |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
title_sort |
Double-hit lymphomas: Clinical, morphological, immunohistochemical and cytogenetic study in a series of Brazilian patients with high-grade non-Hodgkin lymphoma |
author |
Oliveira, Cristiano Claudino [UNESP] |
author_facet |
Oliveira, Cristiano Claudino [UNESP] Maciel-Guerra, Helena [UNESP] Kucko, Luan [UNESP] Hirama, Eric Jun [UNESP] Brilhante, Am�rico Delgado Quevedo, Francisco Carlos da Cunha, Isabela Werneck Soares, Fernando Augusto Niero-Melo, Ligia [UNESP] dos Reis, Patr�cia Pintor [UNESP] Domingues, Maria Aparecida Custodio [UNESP] |
author_role |
author |
author2 |
Maciel-Guerra, Helena [UNESP] Kucko, Luan [UNESP] Hirama, Eric Jun [UNESP] Brilhante, Am�rico Delgado Quevedo, Francisco Carlos da Cunha, Isabela Werneck Soares, Fernando Augusto Niero-Melo, Ligia [UNESP] dos Reis, Patr�cia Pintor [UNESP] Domingues, Maria Aparecida Custodio [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) AC Camargo Cancer Center Amaral Carvalho Hospital |
dc.contributor.author.fl_str_mv |
Oliveira, Cristiano Claudino [UNESP] Maciel-Guerra, Helena [UNESP] Kucko, Luan [UNESP] Hirama, Eric Jun [UNESP] Brilhante, Am�rico Delgado Quevedo, Francisco Carlos da Cunha, Isabela Werneck Soares, Fernando Augusto Niero-Melo, Ligia [UNESP] dos Reis, Patr�cia Pintor [UNESP] Domingues, Maria Aparecida Custodio [UNESP] |
dc.subject.por.fl_str_mv |
Cytogenetics Immunohistochemistry In situ hybridization fluorescence Lymphoma Lymphoma, non-Hodgkin |
topic |
Cytogenetics Immunohistochemistry In situ hybridization fluorescence Lymphoma Lymphoma, non-Hodgkin |
description |
Background: Double-hit lymphomas (DHL) are rare high-grade neoplasms characterized by two translocations: one involving the gene MYC and another involving genes BCL2 or BCL6, whose diagnosis depends on cytogenetic examination. This research studied DHL and morphological and/or immunophenotypic factors associated with the detection of these translocations in a group of high-grade non-Hodgkin lymphoma cases. Method: Clinical and morphological reviews of 120 cases diagnosed with diffuse large B-cell lymphoma and Burkitt lymphoma were conducted. Immunohistochemistry (CD20, CD79a, PAX5, CD10, Bcl6, Bcl2, MUM1, TDT and Myc) and fluorescence in situ hybridization for detection of MYC, BCL2 and BCL6 gene translocations were performed in a tissue microarray platform. Results: Three cases of DHL were detected: two with translocations of MYC and BCL2 and one with translocations of MYC and BCL6, all leading to death in less than six months. Among 90 cytogenetically evaluable biopsies, associations were determined between immunohistochemistry and fluorescence in situ hybridization for MYC (p = 0.036) and BCL2 (p = 0.001). However, these showed only regular agreement, indicated by Kappa values of 0.23 [0.0;0.49] and 0.35 [0.13;0.56], respectively. Starry sky morphology was strongly associated with MYC positivity (p = 0.01). The detection of three cases of DHL, all resulting in death, confirms the rarity and aggressiveness of this neoplasm. Conclusions: The starry sky morphological pattern and immunohistochemical expression of Myc and Bcl2 represent possible selection factors for additional cytogenetic diagnostic testing. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-07 2018-12-11T17:09:24Z 2018-12-11T17:09:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s13000-016-0593-0 Diagnostic Pathology, v. 12, n. 1, 2017. 1746-1596 http://hdl.handle.net/11449/174116 10.1186/s13000-016-0593-0 2-s2.0-85010188987 2-s2.0-85010188987.pdf 0585723113037140 |
url |
http://dx.doi.org/10.1186/s13000-016-0593-0 http://hdl.handle.net/11449/174116 |
identifier_str_mv |
Diagnostic Pathology, v. 12, n. 1, 2017. 1746-1596 10.1186/s13000-016-0593-0 2-s2.0-85010188987 2-s2.0-85010188987.pdf 0585723113037140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diagnostic Pathology 0,818 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021427161595904 |