Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cropro.2022.106071 http://hdl.handle.net/11449/246477 |
Resumo: | The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith, 1797), has evolved resistance to several insecticides and Bacillus thuringiensis Berliner (Bt) toxins expressed in transgenic plants worldwide. Recently, we demonstrated a high risk of resistance evolution of a laboratory-selected emamectin benzoate-resistant strain of FAW based on incomplete dominant inheritance. In this study, we presented laboratory studies that established the baseline susceptibility and defined a diagnostic concentration of emamectin benzoate for the FAW performed in 2002. Then, resistance to emamectin benzoate was monitored in field-collected populations of FAW in Brazil from 2003 to 2004 and 2019 to 2021 using different methods: (i) estimating concentration-mortality curves; (ii) determining larval survival at a diagnostic concentration of emamectin benzoate; and (iii) assessing its survivorship on maize leaves sprayed with the field rate of this insecticide. Populations of FAW presented similar susceptibility to emamectin benzoate in 2002 (LC50 from 0.25 to 0.44 μg a.i./mL) and 2003/04 (LC50 from 0.13 to 0.43 μg a.i./mL), not differing from the susceptible reference population (LC50 of 0.22 μg a.i./mL). From 2003 to 2004, larval survival at the diagnostic concentration of emamectin benzoate of 3.2 μg a.i./mL was <1.79%. From 2019 to 2021, the susceptibility to emamectin benzoate in field populations of FAW decreased, with LC50 values ranging from 0.78 to 158.0 μg a.i./mL, indicating resistance ratios up to 632-fold. Larval survival at a diagnostic concentration of emamectin benzoate also increased, reaching up to 81.4%. Larval survival on maize leaves sprayed with the field rate of emamectin benzoate ranged from 16.6 to 88.5% in populations of FAW collected in 2021. Overall, our findings revealed significant shifts towards decrease in the susceptibility to emamectin benzoate in Brazilian populations of FAW, providing robust evidence of field-evolved resistance. |
id |
UNSP_dc9cfdd5ed465d6d8403028c6c03ffe1 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/246477 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in BrazilFall armywormGlutamate-gated chloride channel allosteric modulatorResistance managementSusceptibilityThe fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith, 1797), has evolved resistance to several insecticides and Bacillus thuringiensis Berliner (Bt) toxins expressed in transgenic plants worldwide. Recently, we demonstrated a high risk of resistance evolution of a laboratory-selected emamectin benzoate-resistant strain of FAW based on incomplete dominant inheritance. In this study, we presented laboratory studies that established the baseline susceptibility and defined a diagnostic concentration of emamectin benzoate for the FAW performed in 2002. Then, resistance to emamectin benzoate was monitored in field-collected populations of FAW in Brazil from 2003 to 2004 and 2019 to 2021 using different methods: (i) estimating concentration-mortality curves; (ii) determining larval survival at a diagnostic concentration of emamectin benzoate; and (iii) assessing its survivorship on maize leaves sprayed with the field rate of this insecticide. Populations of FAW presented similar susceptibility to emamectin benzoate in 2002 (LC50 from 0.25 to 0.44 μg a.i./mL) and 2003/04 (LC50 from 0.13 to 0.43 μg a.i./mL), not differing from the susceptible reference population (LC50 of 0.22 μg a.i./mL). From 2003 to 2004, larval survival at the diagnostic concentration of emamectin benzoate of 3.2 μg a.i./mL was <1.79%. From 2019 to 2021, the susceptibility to emamectin benzoate in field populations of FAW decreased, with LC50 values ranging from 0.78 to 158.0 μg a.i./mL, indicating resistance ratios up to 632-fold. Larval survival at a diagnostic concentration of emamectin benzoate also increased, reaching up to 81.4%. Larval survival on maize leaves sprayed with the field rate of emamectin benzoate ranged from 16.6 to 88.5% in populations of FAW collected in 2021. Overall, our findings revealed significant shifts towards decrease in the susceptibility to emamectin benzoate in Brazilian populations of FAW, providing robust evidence of field-evolved resistance.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Entomology and Acarology University of São Paulo (USP), Av. Pádua Dias 11, PiracicabaInstitute of Biosciences São Paulo State University (UNESP), BotucatuDepartment of Plant Protection Federal University of Santa Maria (UFSM), Rio Grande do SulInstitute of Biosciences São Paulo State University (UNESP), BotucatuFAPESP: 2019/20385-0CNPq: 305464/2020-0CNPq: 314160/2020-5Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de Sergipe (UFS)Muraro, Dionei S.Salmeron, EloisaCruz, João V.S.Amaral, Fernando S.A.Guidolin, Aline S.Nascimento, Antonio R.B.Malaquias, José B. [UNESP]Bernardi, OderleiOmoto, Celso2023-07-29T12:41:58Z2023-07-29T12:41:58Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cropro.2022.106071Crop Protection, v. 162.0261-2194http://hdl.handle.net/11449/24647710.1016/j.cropro.2022.1060712-s2.0-85144007821Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCrop Protectioninfo:eu-repo/semantics/openAccess2023-07-29T12:41:58Zoai:repositorio.unesp.br:11449/246477Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:57:59.693597Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
title |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
spellingShingle |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil Muraro, Dionei S. Fall armyworm Glutamate-gated chloride channel allosteric modulator Resistance management Susceptibility |
title_short |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
title_full |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
title_fullStr |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
title_full_unstemmed |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
title_sort |
Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil |
author |
Muraro, Dionei S. |
author_facet |
Muraro, Dionei S. Salmeron, Eloisa Cruz, João V.S. Amaral, Fernando S.A. Guidolin, Aline S. Nascimento, Antonio R.B. Malaquias, José B. [UNESP] Bernardi, Oderlei Omoto, Celso |
author_role |
author |
author2 |
Salmeron, Eloisa Cruz, João V.S. Amaral, Fernando S.A. Guidolin, Aline S. Nascimento, Antonio R.B. Malaquias, José B. [UNESP] Bernardi, Oderlei Omoto, Celso |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Federal de Sergipe (UFS) |
dc.contributor.author.fl_str_mv |
Muraro, Dionei S. Salmeron, Eloisa Cruz, João V.S. Amaral, Fernando S.A. Guidolin, Aline S. Nascimento, Antonio R.B. Malaquias, José B. [UNESP] Bernardi, Oderlei Omoto, Celso |
dc.subject.por.fl_str_mv |
Fall armyworm Glutamate-gated chloride channel allosteric modulator Resistance management Susceptibility |
topic |
Fall armyworm Glutamate-gated chloride channel allosteric modulator Resistance management Susceptibility |
description |
The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith, 1797), has evolved resistance to several insecticides and Bacillus thuringiensis Berliner (Bt) toxins expressed in transgenic plants worldwide. Recently, we demonstrated a high risk of resistance evolution of a laboratory-selected emamectin benzoate-resistant strain of FAW based on incomplete dominant inheritance. In this study, we presented laboratory studies that established the baseline susceptibility and defined a diagnostic concentration of emamectin benzoate for the FAW performed in 2002. Then, resistance to emamectin benzoate was monitored in field-collected populations of FAW in Brazil from 2003 to 2004 and 2019 to 2021 using different methods: (i) estimating concentration-mortality curves; (ii) determining larval survival at a diagnostic concentration of emamectin benzoate; and (iii) assessing its survivorship on maize leaves sprayed with the field rate of this insecticide. Populations of FAW presented similar susceptibility to emamectin benzoate in 2002 (LC50 from 0.25 to 0.44 μg a.i./mL) and 2003/04 (LC50 from 0.13 to 0.43 μg a.i./mL), not differing from the susceptible reference population (LC50 of 0.22 μg a.i./mL). From 2003 to 2004, larval survival at the diagnostic concentration of emamectin benzoate of 3.2 μg a.i./mL was <1.79%. From 2019 to 2021, the susceptibility to emamectin benzoate in field populations of FAW decreased, with LC50 values ranging from 0.78 to 158.0 μg a.i./mL, indicating resistance ratios up to 632-fold. Larval survival at a diagnostic concentration of emamectin benzoate also increased, reaching up to 81.4%. Larval survival on maize leaves sprayed with the field rate of emamectin benzoate ranged from 16.6 to 88.5% in populations of FAW collected in 2021. Overall, our findings revealed significant shifts towards decrease in the susceptibility to emamectin benzoate in Brazilian populations of FAW, providing robust evidence of field-evolved resistance. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T12:41:58Z 2023-07-29T12:41:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cropro.2022.106071 Crop Protection, v. 162. 0261-2194 http://hdl.handle.net/11449/246477 10.1016/j.cropro.2022.106071 2-s2.0-85144007821 |
url |
http://dx.doi.org/10.1016/j.cropro.2022.106071 http://hdl.handle.net/11449/246477 |
identifier_str_mv |
Crop Protection, v. 162. 0261-2194 10.1016/j.cropro.2022.106071 2-s2.0-85144007821 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Crop Protection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129378799845376 |