Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane

Detalhes bibliográficos
Autor(a) principal: Tupinambá, Rogerio Amaral
Data de Publicação: 2017
Outros Autores: Claro, Cristiane Aparecida De Assis, Pereira, Cristiane Aparecida, Nobrega, Celestino José Prudente, Claro, Ana Paula Rosifini Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar
http://hdl.handle.net/11449/158223
Resumo: ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film.
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spelling Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxaneOrthodontic bracketsBacterial adhesionHexamethyldisiloxane.Braquetes ortodônticosAdesão bacterianaHexametildissiloxano.ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film.RESUMOIntrodução:a deposição de filme de polímero a plasma foi criada para modificar as propriedades de superfície dos braquetes ortodônticos metálicos, com o intuito de inibir a adesão bacteriana.Métodos: filmes finos de polímero de hexametildisiloxano (HMDSO) foram depositados em braquetes ortodônticos de aço inoxidável convencionais (n = 10) e autoligáveis (n = 10), utilizando a técnica de radiofrequência PECVD (Plasma-Enhanced Chemical Vapor Deposition). As amostras foram divididas em dois grupos, de acordo com o tipo de braquete, e dois subgrupos após o tratamento de superfície. A microscopia eletrônica de varredura (MEV) foi realizada para avaliar a presença de adesão bacteriana sobre as superfícies das amostras (região de ranhura horizontal e aletas) e a integridade da camada de filme. A Interferometria Confocal (CI) avaliou a rugosidade, e a molhabilidade superficial foi avaliada por goniometria. Para análise de adesão bacteriana, as amostras foram expostas durante 72 horas a uma solução de Streptococcus mutans, para formação de biofilme. Os valores obtidos para a rugosidade da superfície foram analisados pelo teste de Mann-Whitney, enquanto a adesão do biofilme foi avaliada pelos testes de Kruskal-Wallis e SNK.Resultados: observaram-se diferenças estatisticamente significativas (p <0,05) para a rugosidade superficial e redução da adesão bacteriana em braquetes convencionais após o tratamento da superfície, e entre braquetes convencionais e autoligáveis. Não foram observadas diferenças estatísticas significativas entre os grupos autoligáveis (p> 0,05).Conclusão: a deposição de polímero a plasma só foi efetiva na redução da rugosidade superficial e adesão bacteriana em braquetes convencionais. Observou-se, também, que os braquetes convencionais apresentaram menor adesão ao biofilme do que os braquetes autoligáveis, apesar da ausência de filme.Universidade Estadual Paulista Faculdade de Engenharia Departamento de MateriaisUniversidade de Taubaté Faculdade de Odontologia Departamento de OrtodontiaUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Microbiologia e ImunologiaNew York University College of Dentistry Linhart Continuing Education ProgramUniversidade Estadual Paulista Faculdade de Engenharia Departamento de MateriaisUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Microbiologia e ImunologiaDental Press InternationalUniversidade Estadual Paulista (Unesp)Universidade de Taubaté Faculdade de Odontologia Departamento de OrtodontiaNew York University College of Dentistry Linhart Continuing Education ProgramTupinambá, Rogerio AmaralClaro, Cristiane Aparecida De AssisPereira, Cristiane AparecidaNobrega, Celestino José PrudenteClaro, Ana Paula Rosifini Alves2018-11-12T17:28:54Z2018-11-12T17:28:54Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article77-85application/pdfhttp://dx.doi.org/10.1590/2177-6709.22.4.077-085.oarDental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017.2176-9451http://hdl.handle.net/11449/15822310.1590/2177-6709.22.4.077-085.oarS2176-94512017000400077S2176-94512017000400077.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Press Journal of Orthodontics0,489info:eu-repo/semantics/openAccess2023-11-03T06:08:05Zoai:repositorio.unesp.br:11449/158223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:47:34.904005Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
title Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
spellingShingle Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
Tupinambá, Rogerio Amaral
Orthodontic brackets
Bacterial adhesion
Hexamethyldisiloxane.
Braquetes ortodônticos
Adesão bacteriana
Hexametildissiloxano.
title_short Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
title_full Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
title_fullStr Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
title_full_unstemmed Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
title_sort Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
author Tupinambá, Rogerio Amaral
author_facet Tupinambá, Rogerio Amaral
Claro, Cristiane Aparecida De Assis
Pereira, Cristiane Aparecida
Nobrega, Celestino José Prudente
Claro, Ana Paula Rosifini Alves
author_role author
author2 Claro, Cristiane Aparecida De Assis
Pereira, Cristiane Aparecida
Nobrega, Celestino José Prudente
Claro, Ana Paula Rosifini Alves
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de Taubaté Faculdade de Odontologia Departamento de Ortodontia
New York University College of Dentistry Linhart Continuing Education Program
dc.contributor.author.fl_str_mv Tupinambá, Rogerio Amaral
Claro, Cristiane Aparecida De Assis
Pereira, Cristiane Aparecida
Nobrega, Celestino José Prudente
Claro, Ana Paula Rosifini Alves
dc.subject.por.fl_str_mv Orthodontic brackets
Bacterial adhesion
Hexamethyldisiloxane.
Braquetes ortodônticos
Adesão bacteriana
Hexametildissiloxano.
topic Orthodontic brackets
Bacterial adhesion
Hexamethyldisiloxane.
Braquetes ortodônticos
Adesão bacteriana
Hexametildissiloxano.
description ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
2018-11-12T17:28:54Z
2018-11-12T17:28:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar
Dental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017.
2176-9451
http://hdl.handle.net/11449/158223
10.1590/2177-6709.22.4.077-085.oar
S2176-94512017000400077
S2176-94512017000400077.pdf
url http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar
http://hdl.handle.net/11449/158223
identifier_str_mv Dental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017.
2176-9451
10.1590/2177-6709.22.4.077-085.oar
S2176-94512017000400077
S2176-94512017000400077.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dental Press Journal of Orthodontics
0,489
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 77-85
application/pdf
dc.publisher.none.fl_str_mv Dental Press International
publisher.none.fl_str_mv Dental Press International
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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