Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar http://hdl.handle.net/11449/158223 |
Resumo: | ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film. |
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Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxaneOrthodontic bracketsBacterial adhesionHexamethyldisiloxane.Braquetes ortodônticosAdesão bacterianaHexametildissiloxano.ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film.RESUMOIntrodução:a deposição de filme de polímero a plasma foi criada para modificar as propriedades de superfície dos braquetes ortodônticos metálicos, com o intuito de inibir a adesão bacteriana.Métodos: filmes finos de polímero de hexametildisiloxano (HMDSO) foram depositados em braquetes ortodônticos de aço inoxidável convencionais (n = 10) e autoligáveis (n = 10), utilizando a técnica de radiofrequência PECVD (Plasma-Enhanced Chemical Vapor Deposition). As amostras foram divididas em dois grupos, de acordo com o tipo de braquete, e dois subgrupos após o tratamento de superfície. A microscopia eletrônica de varredura (MEV) foi realizada para avaliar a presença de adesão bacteriana sobre as superfícies das amostras (região de ranhura horizontal e aletas) e a integridade da camada de filme. A Interferometria Confocal (CI) avaliou a rugosidade, e a molhabilidade superficial foi avaliada por goniometria. Para análise de adesão bacteriana, as amostras foram expostas durante 72 horas a uma solução de Streptococcus mutans, para formação de biofilme. Os valores obtidos para a rugosidade da superfície foram analisados pelo teste de Mann-Whitney, enquanto a adesão do biofilme foi avaliada pelos testes de Kruskal-Wallis e SNK.Resultados: observaram-se diferenças estatisticamente significativas (p <0,05) para a rugosidade superficial e redução da adesão bacteriana em braquetes convencionais após o tratamento da superfície, e entre braquetes convencionais e autoligáveis. Não foram observadas diferenças estatísticas significativas entre os grupos autoligáveis (p> 0,05).Conclusão: a deposição de polímero a plasma só foi efetiva na redução da rugosidade superficial e adesão bacteriana em braquetes convencionais. Observou-se, também, que os braquetes convencionais apresentaram menor adesão ao biofilme do que os braquetes autoligáveis, apesar da ausência de filme.Universidade Estadual Paulista Faculdade de Engenharia Departamento de MateriaisUniversidade de Taubaté Faculdade de Odontologia Departamento de OrtodontiaUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Microbiologia e ImunologiaNew York University College of Dentistry Linhart Continuing Education ProgramUniversidade Estadual Paulista Faculdade de Engenharia Departamento de MateriaisUniversidade Estadual Paulista Faculdade de Odontologia Departamento de Microbiologia e ImunologiaDental Press InternationalUniversidade Estadual Paulista (Unesp)Universidade de Taubaté Faculdade de Odontologia Departamento de OrtodontiaNew York University College of Dentistry Linhart Continuing Education ProgramTupinambá, Rogerio AmaralClaro, Cristiane Aparecida De AssisPereira, Cristiane AparecidaNobrega, Celestino José PrudenteClaro, Ana Paula Rosifini Alves2018-11-12T17:28:54Z2018-11-12T17:28:54Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article77-85application/pdfhttp://dx.doi.org/10.1590/2177-6709.22.4.077-085.oarDental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017.2176-9451http://hdl.handle.net/11449/15822310.1590/2177-6709.22.4.077-085.oarS2176-94512017000400077S2176-94512017000400077.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Press Journal of Orthodontics0,489info:eu-repo/semantics/openAccess2023-11-03T06:08:05Zoai:repositorio.unesp.br:11449/158223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:47:34.904005Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
title |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
spellingShingle |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane Tupinambá, Rogerio Amaral Orthodontic brackets Bacterial adhesion Hexamethyldisiloxane. Braquetes ortodônticos Adesão bacteriana Hexametildissiloxano. |
title_short |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
title_full |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
title_fullStr |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
title_full_unstemmed |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
title_sort |
Bacterial adhesion on conventional and self-ligating metallic brackets after surface treatment with plasma-polymerized hexamethyldisiloxane |
author |
Tupinambá, Rogerio Amaral |
author_facet |
Tupinambá, Rogerio Amaral Claro, Cristiane Aparecida De Assis Pereira, Cristiane Aparecida Nobrega, Celestino José Prudente Claro, Ana Paula Rosifini Alves |
author_role |
author |
author2 |
Claro, Cristiane Aparecida De Assis Pereira, Cristiane Aparecida Nobrega, Celestino José Prudente Claro, Ana Paula Rosifini Alves |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Taubaté Faculdade de Odontologia Departamento de Ortodontia New York University College of Dentistry Linhart Continuing Education Program |
dc.contributor.author.fl_str_mv |
Tupinambá, Rogerio Amaral Claro, Cristiane Aparecida De Assis Pereira, Cristiane Aparecida Nobrega, Celestino José Prudente Claro, Ana Paula Rosifini Alves |
dc.subject.por.fl_str_mv |
Orthodontic brackets Bacterial adhesion Hexamethyldisiloxane. Braquetes ortodônticos Adesão bacteriana Hexametildissiloxano. |
topic |
Orthodontic brackets Bacterial adhesion Hexamethyldisiloxane. Braquetes ortodônticos Adesão bacteriana Hexametildissiloxano. |
description |
ABSTRACTIntroduction:Plasma-polymerized film deposition was created to modify metallic orthodontic brackets surface properties in order to inhibit bacterial adhesion.Methods:Hexamethyldisiloxane (HMDSO) polymer films were deposited on conventional (n = 10) and self-ligating (n = 10) stainless steel orthodontic brackets using the Plasma-Enhanced Chemical Vapor Deposition (PECVD) radio frequency technique. The samples were divided into two groups according to the kind of bracket and two subgroups after surface treatment. Scanning Electron Microscopy (SEM) analysis was performed to assess the presence of bacterial adhesion over samples surfaces (slot and wings region) and film layer integrity. Surface roughness was assessed by Confocal Interferometry (CI) and surface wettability, by goniometry. For bacterial adhesion analysis, samples were exposed for 72 hours to a Streptococcus mutans solution for biofilm formation. The values obtained for surface roughness were analyzed using the Mann-Whitney test while biofilm adhesion were assessed by Kruskal-Wallis and SNK test.Results:Significant statistical differences (p< 0.05) for surface roughness and bacterial adhesion reduction were observed on conventional brackets after surface treatment and between conventional and self-ligating brackets; no significant statistical differences were observed between self-ligating groups (p> 0.05).Conclusion: Plasma-polymerized film deposition was only effective on reducing surface roughness and bacterial adhesion in conventional brackets. It was also noted that conventional brackets showed lower biofilm adhesion than self-ligating brackets despite the absence of film. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2018-11-12T17:28:54Z 2018-11-12T17:28:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar Dental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017. 2176-9451 http://hdl.handle.net/11449/158223 10.1590/2177-6709.22.4.077-085.oar S2176-94512017000400077 S2176-94512017000400077.pdf |
url |
http://dx.doi.org/10.1590/2177-6709.22.4.077-085.oar http://hdl.handle.net/11449/158223 |
identifier_str_mv |
Dental Press Journal of Orthodontics. Dental Press International, v. 22, n. 4, p. 77-85, 2017. 2176-9451 10.1590/2177-6709.22.4.077-085.oar S2176-94512017000400077 S2176-94512017000400077.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Press Journal of Orthodontics 0,489 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
77-85 application/pdf |
dc.publisher.none.fl_str_mv |
Dental Press International |
publisher.none.fl_str_mv |
Dental Press International |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128701876928512 |