Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232040 |
Resumo: | A new spectrophotometric method employing a micellar medium is proposed for the determination of paracetamol in pharmaceuticals. The method is based on the acid hydrolysis of paracetamol to p-aminophenol (PAP), which under acidic conditions reacts with p-dimethylaminocinnamaldehyde (p-DAC), producing a red compound (λmax = 530 nm). This reaction can be enhanced five-fold in the presence of sodium dodecyl sulfate (SDS). The effects of all the parameters involved in both the hydrolysis step and the derivatization reaction were investigated using experimental design methodologies. The method presented a linear range of 0.2 to 3.9 μg mL-1 and an excellent correlation coefficient (r = 0.9996). The limit of detection was estimated to be 30.0 μg/L. The technique was successfully applied for the determination of paracetamol in commercial brands of pharmaceuticals. No interferences from the excipients commonly used in commercial formulations were observed, and the results obtained compared favorably with measurements made using the United States Pharmacopeia procedure, at a 95% confidence level. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar mediumMicrowavep-dimethylaminocinnamaldehydeParacetamolSpectrophotometrySurfactantA new spectrophotometric method employing a micellar medium is proposed for the determination of paracetamol in pharmaceuticals. The method is based on the acid hydrolysis of paracetamol to p-aminophenol (PAP), which under acidic conditions reacts with p-dimethylaminocinnamaldehyde (p-DAC), producing a red compound (λmax = 530 nm). This reaction can be enhanced five-fold in the presence of sodium dodecyl sulfate (SDS). The effects of all the parameters involved in both the hydrolysis step and the derivatization reaction were investigated using experimental design methodologies. The method presented a linear range of 0.2 to 3.9 μg mL-1 and an excellent correlation coefficient (r = 0.9996). The limit of detection was estimated to be 30.0 μg/L. The technique was successfully applied for the determination of paracetamol in commercial brands of pharmaceuticals. No interferences from the excipients commonly used in commercial formulations were observed, and the results obtained compared favorably with measurements made using the United States Pharmacopeia procedure, at a 95% confidence level.Instituto de Química de Araraquara UNESP Univ. Estadual Paulista, PO Box 355, CEP 14801-970, Araraquara,SPInstituto de Química de Araraquara UNESP Univ. Estadual Paulista, PO Box 355, CEP 14801-970, Araraquara,SPUniversidade Estadual Paulista (UNESP)Sequinel, Rodrigo [UNESP]Rufino, José L. [UNESP]Pezza, Helena R. [UNESP]Pezza, Leonardo [UNESP]2022-04-29T08:48:47Z2022-04-29T08:48:47Z2011-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1330-1335Latin American Journal of Pharmacy, v. 30, n. 7, p. 1330-1335, 2011.0326-2383http://hdl.handle.net/11449/2320402-s2.0-82355165055Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLatin American Journal of Pharmacyinfo:eu-repo/semantics/openAccess2022-04-29T08:48:47Zoai:repositorio.unesp.br:11449/232040Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:49:18.640426Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
title |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
spellingShingle |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium Sequinel, Rodrigo [UNESP] Microwave p-dimethylaminocinnamaldehyde Paracetamol Spectrophotometry Surfactant |
title_short |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
title_full |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
title_fullStr |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
title_full_unstemmed |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
title_sort |
Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium |
author |
Sequinel, Rodrigo [UNESP] |
author_facet |
Sequinel, Rodrigo [UNESP] Rufino, José L. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
author_role |
author |
author2 |
Rufino, José L. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Sequinel, Rodrigo [UNESP] Rufino, José L. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
dc.subject.por.fl_str_mv |
Microwave p-dimethylaminocinnamaldehyde Paracetamol Spectrophotometry Surfactant |
topic |
Microwave p-dimethylaminocinnamaldehyde Paracetamol Spectrophotometry Surfactant |
description |
A new spectrophotometric method employing a micellar medium is proposed for the determination of paracetamol in pharmaceuticals. The method is based on the acid hydrolysis of paracetamol to p-aminophenol (PAP), which under acidic conditions reacts with p-dimethylaminocinnamaldehyde (p-DAC), producing a red compound (λmax = 530 nm). This reaction can be enhanced five-fold in the presence of sodium dodecyl sulfate (SDS). The effects of all the parameters involved in both the hydrolysis step and the derivatization reaction were investigated using experimental design methodologies. The method presented a linear range of 0.2 to 3.9 μg mL-1 and an excellent correlation coefficient (r = 0.9996). The limit of detection was estimated to be 30.0 μg/L. The technique was successfully applied for the determination of paracetamol in commercial brands of pharmaceuticals. No interferences from the excipients commonly used in commercial formulations were observed, and the results obtained compared favorably with measurements made using the United States Pharmacopeia procedure, at a 95% confidence level. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-02 2022-04-29T08:48:47Z 2022-04-29T08:48:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Latin American Journal of Pharmacy, v. 30, n. 7, p. 1330-1335, 2011. 0326-2383 http://hdl.handle.net/11449/232040 2-s2.0-82355165055 |
identifier_str_mv |
Latin American Journal of Pharmacy, v. 30, n. 7, p. 1330-1335, 2011. 0326-2383 2-s2.0-82355165055 |
url |
http://hdl.handle.net/11449/232040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Latin American Journal of Pharmacy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1330-1335 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129361684987904 |