Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0220718 http://hdl.handle.net/11449/186167 |
Resumo: | A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material. |
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Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement propertiesA series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Piracicaba, SP, BrazilUniv Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP, BrazilUniv Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP, BrazilState Univ Sao Paulo, Sch Sci, Dept Phys, Bauru, SP, BrazilUniv Fed Ceara, Dept Restorat Dent, Fortaleza, Ceara, BrazilNYU, Coll Dent, Dept Epidemiol & Hlth Promot, New York, NY USAUniv Sao Paulo, Super Sch Agr Luiz De Queiroz, Tech Sect Informat Technol, Piracicaba, SP, BrazilPurdue Univ Indianapolis, Indiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN USAUniv Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP, BrazilState Univ Sao Paulo, Sch Sci, Dept Phys, Bauru, SP, BrazilFAPESP: 2014/01723-9FAPESP: 2015/08871-6CNPq: 443036/2014-4CNPq: 310522/2015-3CNPq: 312868/2017-0Public Library ScienceUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Univ Fed CearaNYUUniversidade de São Paulo (USP)Purdue Univ IndianapolisFreire de Castilho, Aline RogeriaRosalen, Pedro LuizSouza Araujo, Isaac Jordao deKitagawa, Igor Lebedenco [UNESP]Goncalves de Araujo Costa, Cecilia AtemJanal, Malvin N.Alves, Marcelo CorreaDuarte, SimoneLisboa Filho, Paulo Noronha [UNESP]Stipp, Rafael NobregaPuppin-Rontani, Regina Maria2019-10-04T12:42:22Z2019-10-04T12:42:22Z2019-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16http://dx.doi.org/10.1371/journal.pone.0220718Plos One. San Francisco: Public Library Science, v. 14, n. 8, 16 p., 2019.1932-6203http://hdl.handle.net/11449/18616710.1371/journal.pone.0220718WOS:00048502680001213538624145320050000-0002-7734-4069Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2024-04-25T17:39:26Zoai:repositorio.unesp.br:11449/186167Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-25T17:39:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
title |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
spellingShingle |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties Freire de Castilho, Aline Rogeria |
title_short |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
title_full |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
title_fullStr |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
title_full_unstemmed |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
title_sort |
Trans, trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties |
author |
Freire de Castilho, Aline Rogeria |
author_facet |
Freire de Castilho, Aline Rogeria Rosalen, Pedro Luiz Souza Araujo, Isaac Jordao de Kitagawa, Igor Lebedenco [UNESP] Goncalves de Araujo Costa, Cecilia Atem Janal, Malvin N. Alves, Marcelo Correa Duarte, Simone Lisboa Filho, Paulo Noronha [UNESP] Stipp, Rafael Nobrega Puppin-Rontani, Regina Maria |
author_role |
author |
author2 |
Rosalen, Pedro Luiz Souza Araujo, Isaac Jordao de Kitagawa, Igor Lebedenco [UNESP] Goncalves de Araujo Costa, Cecilia Atem Janal, Malvin N. Alves, Marcelo Correa Duarte, Simone Lisboa Filho, Paulo Noronha [UNESP] Stipp, Rafael Nobrega Puppin-Rontani, Regina Maria |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Univ Fed Ceara NYU Universidade de São Paulo (USP) Purdue Univ Indianapolis |
dc.contributor.author.fl_str_mv |
Freire de Castilho, Aline Rogeria Rosalen, Pedro Luiz Souza Araujo, Isaac Jordao de Kitagawa, Igor Lebedenco [UNESP] Goncalves de Araujo Costa, Cecilia Atem Janal, Malvin N. Alves, Marcelo Correa Duarte, Simone Lisboa Filho, Paulo Noronha [UNESP] Stipp, Rafael Nobrega Puppin-Rontani, Regina Maria |
description |
A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:42:22Z 2019-10-04T12:42:22Z 2019-08-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0220718 Plos One. San Francisco: Public Library Science, v. 14, n. 8, 16 p., 2019. 1932-6203 http://hdl.handle.net/11449/186167 10.1371/journal.pone.0220718 WOS:000485026800012 1353862414532005 0000-0002-7734-4069 |
url |
http://dx.doi.org/10.1371/journal.pone.0220718 http://hdl.handle.net/11449/186167 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 14, n. 8, 16 p., 2019. 1932-6203 10.1371/journal.pone.0220718 WOS:000485026800012 1353862414532005 0000-0002-7734-4069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964563308806144 |