Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.diagmicrobio.2022.115728 http://hdl.handle.net/11449/241255 |
Resumo: | Objective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84–0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80–0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling16S rRNA geneaerobic vaginitisbacterial vaginosismicroscopymolecular diagnosticsvaginal microbiotaObjective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84–0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80–0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa de Pós-Graduação em Tocoginecologia e Saúde da Mulher University of Paraná (UFPR), ParanáInstitute for Genome Sciences Department of Microbiology and Immunology University of Maryland School of MedicineUniversity of Paraná (UFPR) Sector of Medical Sciences Department of Gynecology and Obstetrics, ParanáSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, São PauloFederal University of Paraná (UFPR) Sector of Biological Sciences Department of Basic Pathology, ParanáSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, São PauloFAPESP: 2012/10403-2FAPESP: 2012/16800-3FAPESP: 2014/22463-5Universidade Federal do Paraná (UFPR)University of Maryland School of MedicineUniversidade Estadual Paulista (UNESP)Durski, MarianaRavel, JacquesSpautz, Ana C.P.G.Carvalho, Newton S.Silva, Márcia G. [UNESP]Marconi, Camila [UNESP]2023-03-01T20:53:51Z2023-03-01T20:53:51Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.diagmicrobio.2022.115728Diagnostic Microbiology and Infectious Disease, v. 104, n. 1, 2022.1879-00700732-8893http://hdl.handle.net/11449/24125510.1016/j.diagmicrobio.2022.1157282-s2.0-85133154338Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Microbiology and Infectious Diseaseinfo:eu-repo/semantics/openAccess2023-03-01T20:53:51Zoai:repositorio.unesp.br:11449/241255Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:53:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
title |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
spellingShingle |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling Durski, Mariana 16S rRNA gene aerobic vaginitis bacterial vaginosis microscopy molecular diagnostics vaginal microbiota |
title_short |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
title_full |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
title_fullStr |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
title_full_unstemmed |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
title_sort |
Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling |
author |
Durski, Mariana |
author_facet |
Durski, Mariana Ravel, Jacques Spautz, Ana C.P.G. Carvalho, Newton S. Silva, Márcia G. [UNESP] Marconi, Camila [UNESP] |
author_role |
author |
author2 |
Ravel, Jacques Spautz, Ana C.P.G. Carvalho, Newton S. Silva, Márcia G. [UNESP] Marconi, Camila [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) University of Maryland School of Medicine Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Durski, Mariana Ravel, Jacques Spautz, Ana C.P.G. Carvalho, Newton S. Silva, Márcia G. [UNESP] Marconi, Camila [UNESP] |
dc.subject.por.fl_str_mv |
16S rRNA gene aerobic vaginitis bacterial vaginosis microscopy molecular diagnostics vaginal microbiota |
topic |
16S rRNA gene aerobic vaginitis bacterial vaginosis microscopy molecular diagnostics vaginal microbiota |
description |
Objective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84–0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80–0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-01T20:53:51Z 2023-03-01T20:53:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115728 Diagnostic Microbiology and Infectious Disease, v. 104, n. 1, 2022. 1879-0070 0732-8893 http://hdl.handle.net/11449/241255 10.1016/j.diagmicrobio.2022.115728 2-s2.0-85133154338 |
url |
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115728 http://hdl.handle.net/11449/241255 |
identifier_str_mv |
Diagnostic Microbiology and Infectious Disease, v. 104, n. 1, 2022. 1879-0070 0732-8893 10.1016/j.diagmicrobio.2022.115728 2-s2.0-85133154338 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diagnostic Microbiology and Infectious Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964755320897536 |