Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/agronomy12061395 http://hdl.handle.net/11449/241944 |
Resumo: | Biological control is considered the only viable integrated disease management practice for controlling the widely distributed and destructive foliar blight and sudden death disease caused by the fungus Rhizoctonia solani AG-1 IA on signal grass (Urochloa brizantha) pastures. Since major signal grass varieties are highly susceptible and fungicide sprays are not labeled for grass pasture cropping systems, biological control is sought as an alternative for managing this fungal disease. In this study, 24 fluorescent Pseudomonas isolates obtained from naturally suppressive soils from the Amazon biome were bio-prospected for their role as biocontrol agents against R. solani AG-1 IA. Based on in vitro antagonism, three isolates (Amana, Poti, and Yara) were selected for further in vivo assays. Multilocus phylogenetic analysis indicated that Amana and Yara were grouped into the Pseudomonas putida group while Poti was grouped into the Pseudomonas asplenii group, and could well constitute a new Pseudomonas species. For in vivo biocontrol assays, the biocontrol agents were applied either via seed-treatment or via foliar spray. All three isolates produced siderophores and solubilized phosphate, while Amana and Poti showed protease and chitinase in vitro activity. Foliar application of P. putida Amana from Amazonian suppressive soils resulted in a significant reduction of the foliar blight disease severity on signal grass. We discuss further steps for the development and labeling of Pseudomonas-based biofungicides for managing the foliar blight disease on signal grass pastures in Brazil. |
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Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blightbiological controlintegrated managementphylogenetic analysisRhizoctonia solani AG-1 IAsudden deathBiological control is considered the only viable integrated disease management practice for controlling the widely distributed and destructive foliar blight and sudden death disease caused by the fungus Rhizoctonia solani AG-1 IA on signal grass (Urochloa brizantha) pastures. Since major signal grass varieties are highly susceptible and fungicide sprays are not labeled for grass pasture cropping systems, biological control is sought as an alternative for managing this fungal disease. In this study, 24 fluorescent Pseudomonas isolates obtained from naturally suppressive soils from the Amazon biome were bio-prospected for their role as biocontrol agents against R. solani AG-1 IA. Based on in vitro antagonism, three isolates (Amana, Poti, and Yara) were selected for further in vivo assays. Multilocus phylogenetic analysis indicated that Amana and Yara were grouped into the Pseudomonas putida group while Poti was grouped into the Pseudomonas asplenii group, and could well constitute a new Pseudomonas species. For in vivo biocontrol assays, the biocontrol agents were applied either via seed-treatment or via foliar spray. All three isolates produced siderophores and solubilized phosphate, while Amana and Poti showed protease and chitinase in vitro activity. Foliar application of P. putida Amana from Amazonian suppressive soils resulted in a significant reduction of the foliar blight disease severity on signal grass. We discuss further steps for the development and labeling of Pseudomonas-based biofungicides for managing the foliar blight disease on signal grass pastures in Brazil.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Crop Protection Agricultural Engineering and Soil (Departamento de Fitossanidade Engenharia Rural e Solos) UNESP—São Paulo State UniversityBiomass Innovation Institute SENAIFederal Institute of Mato Grosso IFMTDepartment of Plant Pathology UFLA Federal University of LavrasDepartment of Crop Protection Agricultural Engineering and Soil (Departamento de Fitossanidade Engenharia Rural e Solos) UNESP—São Paulo State UniversityFAPESP: 11/23050-8FAPESP: 11/50150-3FAPESP: 13/11944-0FAPESP: 17/03403-Universidade Estadual Paulista (UNESP)SENAIIFMTUniversidade Federal de Lavras (UFLA)Vicentini, Samara Nunes Campos [UNESP]Carvalho, Giselle deKrug, Loane Dantas [UNESP]Nunes, Tiago Calvesda Silva, Abimael Gomes [UNESP]Moreira, Silvino IntraGonçalves, Lucas Matheus de Deus Paes [UNESP]Silva, Tatiane Carla [UNESP]Ceresini, Paulo Cezar [UNESP]2023-03-02T04:42:58Z2023-03-02T04:42:58Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/agronomy12061395Agronomy, v. 12, n. 6, 2022.2073-4395http://hdl.handle.net/11449/24194410.3390/agronomy120613952-s2.0-85132103976Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAgronomyinfo:eu-repo/semantics/openAccess2023-03-02T04:42:58Zoai:repositorio.unesp.br:11449/241944Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T04:42:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
title |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
spellingShingle |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight Vicentini, Samara Nunes Campos [UNESP] biological control integrated management phylogenetic analysis Rhizoctonia solani AG-1 IA sudden death |
title_short |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
title_full |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
title_fullStr |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
title_full_unstemmed |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
title_sort |
Bioprospecting Fluorescent Pseudomonas from the Brazilian Amazon for the Biocontrol of Signal Grass Foliar Blight |
author |
Vicentini, Samara Nunes Campos [UNESP] |
author_facet |
Vicentini, Samara Nunes Campos [UNESP] Carvalho, Giselle de Krug, Loane Dantas [UNESP] Nunes, Tiago Calves da Silva, Abimael Gomes [UNESP] Moreira, Silvino Intra Gonçalves, Lucas Matheus de Deus Paes [UNESP] Silva, Tatiane Carla [UNESP] Ceresini, Paulo Cezar [UNESP] |
author_role |
author |
author2 |
Carvalho, Giselle de Krug, Loane Dantas [UNESP] Nunes, Tiago Calves da Silva, Abimael Gomes [UNESP] Moreira, Silvino Intra Gonçalves, Lucas Matheus de Deus Paes [UNESP] Silva, Tatiane Carla [UNESP] Ceresini, Paulo Cezar [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) SENAI IFMT Universidade Federal de Lavras (UFLA) |
dc.contributor.author.fl_str_mv |
Vicentini, Samara Nunes Campos [UNESP] Carvalho, Giselle de Krug, Loane Dantas [UNESP] Nunes, Tiago Calves da Silva, Abimael Gomes [UNESP] Moreira, Silvino Intra Gonçalves, Lucas Matheus de Deus Paes [UNESP] Silva, Tatiane Carla [UNESP] Ceresini, Paulo Cezar [UNESP] |
dc.subject.por.fl_str_mv |
biological control integrated management phylogenetic analysis Rhizoctonia solani AG-1 IA sudden death |
topic |
biological control integrated management phylogenetic analysis Rhizoctonia solani AG-1 IA sudden death |
description |
Biological control is considered the only viable integrated disease management practice for controlling the widely distributed and destructive foliar blight and sudden death disease caused by the fungus Rhizoctonia solani AG-1 IA on signal grass (Urochloa brizantha) pastures. Since major signal grass varieties are highly susceptible and fungicide sprays are not labeled for grass pasture cropping systems, biological control is sought as an alternative for managing this fungal disease. In this study, 24 fluorescent Pseudomonas isolates obtained from naturally suppressive soils from the Amazon biome were bio-prospected for their role as biocontrol agents against R. solani AG-1 IA. Based on in vitro antagonism, three isolates (Amana, Poti, and Yara) were selected for further in vivo assays. Multilocus phylogenetic analysis indicated that Amana and Yara were grouped into the Pseudomonas putida group while Poti was grouped into the Pseudomonas asplenii group, and could well constitute a new Pseudomonas species. For in vivo biocontrol assays, the biocontrol agents were applied either via seed-treatment or via foliar spray. All three isolates produced siderophores and solubilized phosphate, while Amana and Poti showed protease and chitinase in vitro activity. Foliar application of P. putida Amana from Amazonian suppressive soils resulted in a significant reduction of the foliar blight disease severity on signal grass. We discuss further steps for the development and labeling of Pseudomonas-based biofungicides for managing the foliar blight disease on signal grass pastures in Brazil. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-02T04:42:58Z 2023-03-02T04:42:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/agronomy12061395 Agronomy, v. 12, n. 6, 2022. 2073-4395 http://hdl.handle.net/11449/241944 10.3390/agronomy12061395 2-s2.0-85132103976 |
url |
http://dx.doi.org/10.3390/agronomy12061395 http://hdl.handle.net/11449/241944 |
identifier_str_mv |
Agronomy, v. 12, n. 6, 2022. 2073-4395 10.3390/agronomy12061395 2-s2.0-85132103976 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Agronomy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965077031354368 |