Innovative molecular approach to the identification of Colossoma macropomum and its hybrids
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012005000025&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/134888 |
Resumo: | Tambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as “Tambacu” and “Tambatinga”. Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (a-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the maternal lineage of the specimen, all of which corresponded to C. macropomum. Unexpectedly, only two CR haplotype were found in 93 samples, a very low genetic diversity for the pisciculture of Tambaqui. Multiplex PCR identified 42 hybrids, in contrast with 23 identified by the supplier on the basis of external morphology. This innovative tool has considerable potential for the development of the Brazilian aquaculture, given the possibility of the systematic identification of the genetic traits of both fry-producing stocks, and the fry and juveniles raised in farms. |
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Innovative molecular approach to the identification of Colossoma macropomum and its hybridsPiscicultureMultiplex PCRMitochondrial DNAGenetic bottleneckTambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as “Tambacu” and “Tambatinga”. Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (a-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the maternal lineage of the specimen, all of which corresponded to C. macropomum. Unexpectedly, only two CR haplotype were found in 93 samples, a very low genetic diversity for the pisciculture of Tambaqui. Multiplex PCR identified 42 hybrids, in contrast with 23 identified by the supplier on the basis of external morphology. This innovative tool has considerable potential for the development of the Brazilian aquaculture, given the possibility of the systematic identification of the genetic traits of both fry-producing stocks, and the fry and juveniles raised in farms.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Faculdade de Ciências de Bauru, Bauru, Engenheiro Luiz Edmundo C. Coube, 14-01, Vargem Limpa, CEP 17033-360, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Faculdade de Ciências de Bauru, Bauru, Engenheiro Luiz Edmundo C. Coube, 14-01, Vargem Limpa, CEP 17033-360, SP, BrasilUniversidade Federal do Pará (UFP)Universidade Estadual do MaranhãoUniversidade Estadual Paulista (Unesp)Gomes, FátimaSchneider, HoracioBarros, ClaudeneSampaio, DionisoHashimoto, Diogo TeruoPorto-Foresti, Fábio [UNESP]Sampaio, Maria Iracilda da Cunha2016-03-02T12:58:52Z2016-03-02T12:58:52Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article89-97application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012005000025&lng=en&nrm=iso&tlng=enAnais da Academia Brasileira de Ciências, v. 84, n. 2, p. 89-97, 2012.0001-3765http://hdl.handle.net/11449/134888ISSN0001-3765-2012-84-02-89-97.pdf3815338174165832Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Ciências0.9560,418info:eu-repo/semantics/openAccess2024-04-23T15:23:29Zoai:repositorio.unesp.br:11449/134888Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:58:41.084375Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
title |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
spellingShingle |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids Gomes, Fátima Pisciculture Multiplex PCR Mitochondrial DNA Genetic bottleneck |
title_short |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
title_full |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
title_fullStr |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
title_full_unstemmed |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
title_sort |
Innovative molecular approach to the identification of Colossoma macropomum and its hybrids |
author |
Gomes, Fátima |
author_facet |
Gomes, Fátima Schneider, Horacio Barros, Claudene Sampaio, Dioniso Hashimoto, Diogo Teruo Porto-Foresti, Fábio [UNESP] Sampaio, Maria Iracilda da Cunha |
author_role |
author |
author2 |
Schneider, Horacio Barros, Claudene Sampaio, Dioniso Hashimoto, Diogo Teruo Porto-Foresti, Fábio [UNESP] Sampaio, Maria Iracilda da Cunha |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFP) Universidade Estadual do Maranhão Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes, Fátima Schneider, Horacio Barros, Claudene Sampaio, Dioniso Hashimoto, Diogo Teruo Porto-Foresti, Fábio [UNESP] Sampaio, Maria Iracilda da Cunha |
dc.subject.por.fl_str_mv |
Pisciculture Multiplex PCR Mitochondrial DNA Genetic bottleneck |
topic |
Pisciculture Multiplex PCR Mitochondrial DNA Genetic bottleneck |
description |
Tambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as “Tambacu” and “Tambatinga”. Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (a-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the maternal lineage of the specimen, all of which corresponded to C. macropomum. Unexpectedly, only two CR haplotype were found in 93 samples, a very low genetic diversity for the pisciculture of Tambaqui. Multiplex PCR identified 42 hybrids, in contrast with 23 identified by the supplier on the basis of external morphology. This innovative tool has considerable potential for the development of the Brazilian aquaculture, given the possibility of the systematic identification of the genetic traits of both fry-producing stocks, and the fry and juveniles raised in farms. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2016-03-02T12:58:52Z 2016-03-02T12:58:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012005000025&lng=en&nrm=iso&tlng=en Anais da Academia Brasileira de Ciências, v. 84, n. 2, p. 89-97, 2012. 0001-3765 http://hdl.handle.net/11449/134888 ISSN0001-3765-2012-84-02-89-97.pdf 3815338174165832 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012005000025&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/134888 |
identifier_str_mv |
Anais da Academia Brasileira de Ciências, v. 84, n. 2, p. 89-97, 2012. 0001-3765 ISSN0001-3765-2012-84-02-89-97.pdf 3815338174165832 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciências 0.956 0,418 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
89-97 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128880660185088 |