Molecular characterization of diaporthe citri isolates using faflp technique.

Detalhes bibliográficos
Autor(a) principal: Nozaki, Márcia de Holanda [UNESP]
Data de Publicação: 2006
Outros Autores: Camargo, Margarete [UNESP], Lemos, Eliana Gertrudes Macedo [UNESP], Aukar, Ana Paula de Andrade [UNESP], Barreto, Modesto [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/228558
Resumo: Citriculture is a market in expansion specially in Sao Paulo State, whose importance in the economy is already recognized. Like any other cultivated species, the increase of planted areas contributes to an increase in disease problems. Thus, many diseases affect citrus species, including melanose, caused by Diaporthe citri (Wolf.), to which most commercial varieties are susceptible to the disease. Kno-wledge of the intra-specific diversity is important when selecting varieties for resistance. This study evaluated the genetic variability among Diaporthe citri isolates, obtained from different geographical regions, varieties, and parts of the plant using molecular markers. Amplified Fragment Length Polymorphism (AFLP) markers were used to characterize ten isolates of this pathogen. Genomic DNAs extracted from mycelial mass were used in amplification reactions. fAFLP allowed separation of the isolates into four distinct groups. However, genetic similarity analysis did not group isolates by locati-on, variety or part of the plant.
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spelling Molecular characterization of diaporthe citri isolates using faflp technique.Caracterização molecular através da técnica faflp de isolados de diaporthe citriCitrusGenetic variabilityMelanosePolymorphismCitriculture is a market in expansion specially in Sao Paulo State, whose importance in the economy is already recognized. Like any other cultivated species, the increase of planted areas contributes to an increase in disease problems. Thus, many diseases affect citrus species, including melanose, caused by Diaporthe citri (Wolf.), to which most commercial varieties are susceptible to the disease. Kno-wledge of the intra-specific diversity is important when selecting varieties for resistance. This study evaluated the genetic variability among Diaporthe citri isolates, obtained from different geographical regions, varieties, and parts of the plant using molecular markers. Amplified Fragment Length Polymorphism (AFLP) markers were used to characterize ten isolates of this pathogen. Genomic DNAs extracted from mycelial mass were used in amplification reactions. fAFLP allowed separation of the isolates into four distinct groups. However, genetic similarity analysis did not group isolates by locati-on, variety or part of the plant.Departamento de Fitossanidade FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane km 5, s/noDepartamento de Tecnologia FCAV/UNESPDepartamento de Fitossanidade FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane km 5, s/noDepartamento de Tecnologia FCAV/UNESPUniversidade Estadual Paulista (UNESP)Nozaki, Márcia de Holanda [UNESP]Camargo, Margarete [UNESP]Lemos, Eliana Gertrudes Macedo [UNESP]Aukar, Ana Paula de Andrade [UNESP]Barreto, Modesto [UNESP]2022-04-29T08:27:22Z2022-04-29T08:27:22Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-150Summa Phytopathologica, v. 32, n. 2, p. 147-150, 2006.0100-5405http://hdl.handle.net/11449/2285582-s2.0-85049290575Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporSumma Phytopathologicainfo:eu-repo/semantics/openAccess2024-06-07T15:31:17Zoai:repositorio.unesp.br:11449/228558Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:41.722168Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization of diaporthe citri isolates using faflp technique.
Caracterização molecular através da técnica faflp de isolados de diaporthe citri
title Molecular characterization of diaporthe citri isolates using faflp technique.
spellingShingle Molecular characterization of diaporthe citri isolates using faflp technique.
Nozaki, Márcia de Holanda [UNESP]
Citrus
Genetic variability
Melanose
Polymorphism
title_short Molecular characterization of diaporthe citri isolates using faflp technique.
title_full Molecular characterization of diaporthe citri isolates using faflp technique.
title_fullStr Molecular characterization of diaporthe citri isolates using faflp technique.
title_full_unstemmed Molecular characterization of diaporthe citri isolates using faflp technique.
title_sort Molecular characterization of diaporthe citri isolates using faflp technique.
author Nozaki, Márcia de Holanda [UNESP]
author_facet Nozaki, Márcia de Holanda [UNESP]
Camargo, Margarete [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Aukar, Ana Paula de Andrade [UNESP]
Barreto, Modesto [UNESP]
author_role author
author2 Camargo, Margarete [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Aukar, Ana Paula de Andrade [UNESP]
Barreto, Modesto [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Nozaki, Márcia de Holanda [UNESP]
Camargo, Margarete [UNESP]
Lemos, Eliana Gertrudes Macedo [UNESP]
Aukar, Ana Paula de Andrade [UNESP]
Barreto, Modesto [UNESP]
dc.subject.por.fl_str_mv Citrus
Genetic variability
Melanose
Polymorphism
topic Citrus
Genetic variability
Melanose
Polymorphism
description Citriculture is a market in expansion specially in Sao Paulo State, whose importance in the economy is already recognized. Like any other cultivated species, the increase of planted areas contributes to an increase in disease problems. Thus, many diseases affect citrus species, including melanose, caused by Diaporthe citri (Wolf.), to which most commercial varieties are susceptible to the disease. Kno-wledge of the intra-specific diversity is important when selecting varieties for resistance. This study evaluated the genetic variability among Diaporthe citri isolates, obtained from different geographical regions, varieties, and parts of the plant using molecular markers. Amplified Fragment Length Polymorphism (AFLP) markers were used to characterize ten isolates of this pathogen. Genomic DNAs extracted from mycelial mass were used in amplification reactions. fAFLP allowed separation of the isolates into four distinct groups. However, genetic similarity analysis did not group isolates by locati-on, variety or part of the plant.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2022-04-29T08:27:22Z
2022-04-29T08:27:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Summa Phytopathologica, v. 32, n. 2, p. 147-150, 2006.
0100-5405
http://hdl.handle.net/11449/228558
2-s2.0-85049290575
identifier_str_mv Summa Phytopathologica, v. 32, n. 2, p. 147-150, 2006.
0100-5405
2-s2.0-85049290575
url http://hdl.handle.net/11449/228558
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Summa Phytopathologica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-150
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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