Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=7601 http://hdl.handle.net/11449/72780 |
Resumo: | The degree of genetic and pathologic variation exhibited by a turkey Coronavirus (TCoV) strain was investigated after nine serial passages in 25-day-old turkey embryos obtained from wild broad-breasted bronze breeders. In spite of spleen, liver, kidneys, cloacal bursa and thymus have been collected and analysed, the main histopathological changes were only documented in the intestine sections. Microscopic lesions were characterized as mild enteritis, low degree of enterocyte vacuolization and detachment of the intestinal villous after five consecutive passages and were considered absent in the last passages. Genealogic analysis based on S1 and S2 DNA sequences suggested that Brazilian isolate might be considered as originated from TCoV strains circulating in the United States, as 100% identity with TCoV-Gl strain. Although S1 S2 sequences from each passage revealed no significant point mutations, and no correlation could be speculate between S2 nucleotide changes and pathologic features in infected embryos. This is the first demonstration of wild turkey embryos as a model for TCoV isolation and propagation. |
id |
UNSP_de04053bd235532ae7dacc71fa0de531 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/72780 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder)PathologySpike geneTurkey coronavirusWild turkey speciesThe degree of genetic and pathologic variation exhibited by a turkey Coronavirus (TCoV) strain was investigated after nine serial passages in 25-day-old turkey embryos obtained from wild broad-breasted bronze breeders. In spite of spleen, liver, kidneys, cloacal bursa and thymus have been collected and analysed, the main histopathological changes were only documented in the intestine sections. Microscopic lesions were characterized as mild enteritis, low degree of enterocyte vacuolization and detachment of the intestinal villous after five consecutive passages and were considered absent in the last passages. Genealogic analysis based on S1 and S2 DNA sequences suggested that Brazilian isolate might be considered as originated from TCoV strains circulating in the United States, as 100% identity with TCoV-Gl strain. Although S1 S2 sequences from each passage revealed no significant point mutations, and no correlation could be speculate between S2 nucleotide changes and pathologic features in infected embryos. This is the first demonstration of wild turkey embryos as a model for TCoV isolation and propagation.UNESP- São Paulo State University Laboratory of Virology DAPSA, Faculdade de Medicina Veterinária, Araçatuba, SPCoronavirus Research Group Faculdade de Medicina Veterinária e Zootecnia USP - University of São Paulo, SPUNESP- São Paulo State University Laboratory of Virology DAPSA, Faculdade de Medicina Veterinária, Araçatuba, SPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Gomes, Deriane E. [UNESP]Rosa, Ana C. G. [UNESP]Nobrega, Daniela F. [UNESP]Garcia, Andrea F. [UNESP]Brandão, Paulo E.Cardoso, Tereza C. [UNESP]2014-05-27T11:26:06Z2014-05-27T11:26:06Z2011-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article184-189http://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=7601Brazilian Journal of Veterinary Pathology, v. 4, n. 3, p. 184-189, 2011.1983-0246http://hdl.handle.net/11449/727802-s2.0-82755186193Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathology0,162info:eu-repo/semantics/openAccess2021-10-23T10:41:11Zoai:repositorio.unesp.br:11449/72780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:41:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
title |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
spellingShingle |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) Gomes, Deriane E. [UNESP] Pathology Spike gene Turkey coronavirus Wild turkey species |
title_short |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
title_full |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
title_fullStr |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
title_full_unstemmed |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
title_sort |
Pathological and molecular analysis of Turkey coronavirus replication in captive wild Turkey embryos (broad-breasted bronze breeder) |
author |
Gomes, Deriane E. [UNESP] |
author_facet |
Gomes, Deriane E. [UNESP] Rosa, Ana C. G. [UNESP] Nobrega, Daniela F. [UNESP] Garcia, Andrea F. [UNESP] Brandão, Paulo E. Cardoso, Tereza C. [UNESP] |
author_role |
author |
author2 |
Rosa, Ana C. G. [UNESP] Nobrega, Daniela F. [UNESP] Garcia, Andrea F. [UNESP] Brandão, Paulo E. Cardoso, Tereza C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gomes, Deriane E. [UNESP] Rosa, Ana C. G. [UNESP] Nobrega, Daniela F. [UNESP] Garcia, Andrea F. [UNESP] Brandão, Paulo E. Cardoso, Tereza C. [UNESP] |
dc.subject.por.fl_str_mv |
Pathology Spike gene Turkey coronavirus Wild turkey species |
topic |
Pathology Spike gene Turkey coronavirus Wild turkey species |
description |
The degree of genetic and pathologic variation exhibited by a turkey Coronavirus (TCoV) strain was investigated after nine serial passages in 25-day-old turkey embryos obtained from wild broad-breasted bronze breeders. In spite of spleen, liver, kidneys, cloacal bursa and thymus have been collected and analysed, the main histopathological changes were only documented in the intestine sections. Microscopic lesions were characterized as mild enteritis, low degree of enterocyte vacuolization and detachment of the intestinal villous after five consecutive passages and were considered absent in the last passages. Genealogic analysis based on S1 and S2 DNA sequences suggested that Brazilian isolate might be considered as originated from TCoV strains circulating in the United States, as 100% identity with TCoV-Gl strain. Although S1 S2 sequences from each passage revealed no significant point mutations, and no correlation could be speculate between S2 nucleotide changes and pathologic features in infected embryos. This is the first demonstration of wild turkey embryos as a model for TCoV isolation and propagation. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-01 2014-05-27T11:26:06Z 2014-05-27T11:26:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=7601 Brazilian Journal of Veterinary Pathology, v. 4, n. 3, p. 184-189, 2011. 1983-0246 http://hdl.handle.net/11449/72780 2-s2.0-82755186193 |
url |
http://www.abpv.vet.br/paginas_site/default.asp?P=BJVP&PAG_SEQ=7601 http://hdl.handle.net/11449/72780 |
identifier_str_mv |
Brazilian Journal of Veterinary Pathology, v. 4, n. 3, p. 184-189, 2011. 1983-0246 2-s2.0-82755186193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Pathology 0,162 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
184-189 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789534489935872 |