Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1088/2515-7647/abe02c http://hdl.handle.net/11449/208487 |
Resumo: | Miniaturized environments have emerged as an excellent alternative to evaluate and understand biological mechanisms. These systems are able to simulate macroenvironments with high reproducibility, achieving many results in a short time of analysis. However, microenvironments require specific architectures that can be reached using laser micromachining techniques, such as two-photon polymerization (TPP). This technique has many advantages, allowing the production of environments without shape limitation and with special features. In this work, aided by the TPP technique, we produce different arrays of microstructures, fabricated using acrylate-based materials, in order to evaluate the growth and development of the Komagataeibacter xylinus bacteria, the micro-organism responsible for producing bacterial cellulose (BC), a natural polymer with several biological applications. BC grown in microenvironments presents similar features to those of biofilm formed in macroenvironments, maintaining their attractive properties. In addition, due to the high optical quality and mechanical resistance of the BC matrices, we use these films as flexible substrates in TPP experiments, obtaining promising results for tissue engineering studies. |
id |
UNSP_dea893b0f6c227d43b86464f99bc31f3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/208487 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerizationAcrylate-based materialsBacterial cellulose growthTwo-photon polymerizationMiniaturized environments have emerged as an excellent alternative to evaluate and understand biological mechanisms. These systems are able to simulate macroenvironments with high reproducibility, achieving many results in a short time of analysis. However, microenvironments require specific architectures that can be reached using laser micromachining techniques, such as two-photon polymerization (TPP). This technique has many advantages, allowing the production of environments without shape limitation and with special features. In this work, aided by the TPP technique, we produce different arrays of microstructures, fabricated using acrylate-based materials, in order to evaluate the growth and development of the Komagataeibacter xylinus bacteria, the micro-organism responsible for producing bacterial cellulose (BC), a natural polymer with several biological applications. BC grown in microenvironments presents similar features to those of biofilm formed in macroenvironments, maintaining their attractive properties. In addition, due to the high optical quality and mechanical resistance of the BC matrices, we use these films as flexible substrates in TPP experiments, obtaining promising results for tissue engineering studies.São Carlos Institute of Physics University of São PauloInstitute of Chemistry São Paulo State University (UNESP)Institute of Chemistry São Paulo State University (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Otuka, Adriano J.G.Domeneguetti, Rafael R. [UNESP]Moraes, Jonathas Q.R.Balogh, Debora T.Ribeiro, Sidney J.L. [UNESP]Mendonça, Cleber R.2021-06-25T11:12:58Z2021-06-25T11:12:58Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1088/2515-7647/abe02cJPhys Photonics, v. 3, n. 2, 2021.2515-7647http://hdl.handle.net/11449/20848710.1088/2515-7647/abe02c2-s2.0-85102170496Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJPhys Photonicsinfo:eu-repo/semantics/openAccess2021-10-23T19:02:13Zoai:repositorio.unesp.br:11449/208487Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
title |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
spellingShingle |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization Otuka, Adriano J.G. Acrylate-based materials Bacterial cellulose growth Two-photon polymerization |
title_short |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
title_full |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
title_fullStr |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
title_full_unstemmed |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
title_sort |
Bacterial cellulose growth on 3D acrylate-based microstructures fabricated by two-photon polymerization |
author |
Otuka, Adriano J.G. |
author_facet |
Otuka, Adriano J.G. Domeneguetti, Rafael R. [UNESP] Moraes, Jonathas Q.R. Balogh, Debora T. Ribeiro, Sidney J.L. [UNESP] Mendonça, Cleber R. |
author_role |
author |
author2 |
Domeneguetti, Rafael R. [UNESP] Moraes, Jonathas Q.R. Balogh, Debora T. Ribeiro, Sidney J.L. [UNESP] Mendonça, Cleber R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Otuka, Adriano J.G. Domeneguetti, Rafael R. [UNESP] Moraes, Jonathas Q.R. Balogh, Debora T. Ribeiro, Sidney J.L. [UNESP] Mendonça, Cleber R. |
dc.subject.por.fl_str_mv |
Acrylate-based materials Bacterial cellulose growth Two-photon polymerization |
topic |
Acrylate-based materials Bacterial cellulose growth Two-photon polymerization |
description |
Miniaturized environments have emerged as an excellent alternative to evaluate and understand biological mechanisms. These systems are able to simulate macroenvironments with high reproducibility, achieving many results in a short time of analysis. However, microenvironments require specific architectures that can be reached using laser micromachining techniques, such as two-photon polymerization (TPP). This technique has many advantages, allowing the production of environments without shape limitation and with special features. In this work, aided by the TPP technique, we produce different arrays of microstructures, fabricated using acrylate-based materials, in order to evaluate the growth and development of the Komagataeibacter xylinus bacteria, the micro-organism responsible for producing bacterial cellulose (BC), a natural polymer with several biological applications. BC grown in microenvironments presents similar features to those of biofilm formed in macroenvironments, maintaining their attractive properties. In addition, due to the high optical quality and mechanical resistance of the BC matrices, we use these films as flexible substrates in TPP experiments, obtaining promising results for tissue engineering studies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:12:58Z 2021-06-25T11:12:58Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1088/2515-7647/abe02c JPhys Photonics, v. 3, n. 2, 2021. 2515-7647 http://hdl.handle.net/11449/208487 10.1088/2515-7647/abe02c 2-s2.0-85102170496 |
url |
http://dx.doi.org/10.1088/2515-7647/abe02c http://hdl.handle.net/11449/208487 |
identifier_str_mv |
JPhys Photonics, v. 3, n. 2, 2021. 2515-7647 10.1088/2515-7647/abe02c 2-s2.0-85102170496 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
JPhys Photonics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965296830709760 |