p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma

Detalhes bibliográficos
Autor(a) principal: Rivera-Calderón, Luis G. [UNESP]
Data de Publicação: 2019
Outros Autores: Fonseca-Alves, Carlos E. [UNESP], Kobayashi, Priscila E. [UNESP], Carvalho, Márcio [UNESP], Vasconcelos, Rosemeri O. [UNESP], Laufer-Amorim, Renée [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.rvsc.2018.11.006
http://hdl.handle.net/11449/188391
Resumo: The mTOR/4E-BP1/eIF4E pathway plays important roles in the neoplastic transformation process and in tumour growth. In men, the mTOR/4E-BP1/eIF4E pathway was described as altered in different tumours, including prostate cancer (PC). Apart from humans, the dog is the only species that develops PC with high frequency and is considered a good model for comparative oncology initiatives. Due to limited information on this pathway in canine tumours, this study aimed to investigate mTOR, 4E-BP1 and eIF4E gene and protein expression in canine PC, as well as in metastatic and normal prostatic tissues, and to evaluate the correlations between gene/protein expression and Gleason score (GS) in PC. A total of 35 formalin-fixed paraffin-embedded (FFPE) samples, including 13 of normal prostatic tissue, 17 PC samples and 5 metastasis samples, were evaluated by immunohistochemistry and qPCR. mTOR gene mutation in the kinase domain was also investigated. We identified higher p-mTOR and eIF4E protein levels in canine PC with higher GS values (≥ 8) and a significant positive correlation in expression between these proteins. eIF4E overexpression was observed in metastasis relative to expression in normal samples. Our data suggest that p-mTOR and eIF4E expression is positively correlated with GS in canine PC, similar to the pattern in humans. More studies of the mTOR/4EBP1/eIF4E pathway should be performed to identify possible correlations of the proteins involved with clinical and pathologic findings in canine PC and the roles of these proteins as therapeutic targets for the treatment of canine PC.
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spelling p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinomaDogGleason scoremTOR pathwayOncologyProstate cancerThe mTOR/4E-BP1/eIF4E pathway plays important roles in the neoplastic transformation process and in tumour growth. In men, the mTOR/4E-BP1/eIF4E pathway was described as altered in different tumours, including prostate cancer (PC). Apart from humans, the dog is the only species that develops PC with high frequency and is considered a good model for comparative oncology initiatives. Due to limited information on this pathway in canine tumours, this study aimed to investigate mTOR, 4E-BP1 and eIF4E gene and protein expression in canine PC, as well as in metastatic and normal prostatic tissues, and to evaluate the correlations between gene/protein expression and Gleason score (GS) in PC. A total of 35 formalin-fixed paraffin-embedded (FFPE) samples, including 13 of normal prostatic tissue, 17 PC samples and 5 metastasis samples, were evaluated by immunohistochemistry and qPCR. mTOR gene mutation in the kinase domain was also investigated. We identified higher p-mTOR and eIF4E protein levels in canine PC with higher GS values (≥ 8) and a significant positive correlation in expression between these proteins. eIF4E overexpression was observed in metastasis relative to expression in normal samples. Our data suggest that p-mTOR and eIF4E expression is positively correlated with GS in canine PC, similar to the pattern in humans. More studies of the mTOR/4EBP1/eIF4E pathway should be performed to identify possible correlations of the proteins involved with clinical and pathologic findings in canine PC and the roles of these proteins as therapeutic targets for the treatment of canine PC.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)Department of Veterinary Pathology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (Unesp)CNPq: 443884/2014-5Universidade Estadual Paulista (Unesp)Rivera-Calderón, Luis G. [UNESP]Fonseca-Alves, Carlos E. [UNESP]Kobayashi, Priscila E. [UNESP]Carvalho, Márcio [UNESP]Vasconcelos, Rosemeri O. [UNESP]Laufer-Amorim, Renée [UNESP]2019-10-06T16:06:34Z2019-10-06T16:06:34Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article86-92http://dx.doi.org/10.1016/j.rvsc.2018.11.006Research in Veterinary Science, v. 122, p. 86-92.1532-26610034-5288http://hdl.handle.net/11449/18839110.1016/j.rvsc.2018.11.0062-s2.0-85056889750Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T15:55:03Zoai:repositorio.unesp.br:11449/188391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:47:02.577924Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
title p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
spellingShingle p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
Rivera-Calderón, Luis G. [UNESP]
Dog
Gleason score
mTOR pathway
Oncology
Prostate cancer
title_short p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
title_full p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
title_fullStr p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
title_full_unstemmed p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
title_sort p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma
author Rivera-Calderón, Luis G. [UNESP]
author_facet Rivera-Calderón, Luis G. [UNESP]
Fonseca-Alves, Carlos E. [UNESP]
Kobayashi, Priscila E. [UNESP]
Carvalho, Márcio [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Laufer-Amorim, Renée [UNESP]
author_role author
author2 Fonseca-Alves, Carlos E. [UNESP]
Kobayashi, Priscila E. [UNESP]
Carvalho, Márcio [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Laufer-Amorim, Renée [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rivera-Calderón, Luis G. [UNESP]
Fonseca-Alves, Carlos E. [UNESP]
Kobayashi, Priscila E. [UNESP]
Carvalho, Márcio [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Laufer-Amorim, Renée [UNESP]
dc.subject.por.fl_str_mv Dog
Gleason score
mTOR pathway
Oncology
Prostate cancer
topic Dog
Gleason score
mTOR pathway
Oncology
Prostate cancer
description The mTOR/4E-BP1/eIF4E pathway plays important roles in the neoplastic transformation process and in tumour growth. In men, the mTOR/4E-BP1/eIF4E pathway was described as altered in different tumours, including prostate cancer (PC). Apart from humans, the dog is the only species that develops PC with high frequency and is considered a good model for comparative oncology initiatives. Due to limited information on this pathway in canine tumours, this study aimed to investigate mTOR, 4E-BP1 and eIF4E gene and protein expression in canine PC, as well as in metastatic and normal prostatic tissues, and to evaluate the correlations between gene/protein expression and Gleason score (GS) in PC. A total of 35 formalin-fixed paraffin-embedded (FFPE) samples, including 13 of normal prostatic tissue, 17 PC samples and 5 metastasis samples, were evaluated by immunohistochemistry and qPCR. mTOR gene mutation in the kinase domain was also investigated. We identified higher p-mTOR and eIF4E protein levels in canine PC with higher GS values (≥ 8) and a significant positive correlation in expression between these proteins. eIF4E overexpression was observed in metastasis relative to expression in normal samples. Our data suggest that p-mTOR and eIF4E expression is positively correlated with GS in canine PC, similar to the pattern in humans. More studies of the mTOR/4EBP1/eIF4E pathway should be performed to identify possible correlations of the proteins involved with clinical and pathologic findings in canine PC and the roles of these proteins as therapeutic targets for the treatment of canine PC.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:06:34Z
2019-10-06T16:06:34Z
2019-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.rvsc.2018.11.006
Research in Veterinary Science, v. 122, p. 86-92.
1532-2661
0034-5288
http://hdl.handle.net/11449/188391
10.1016/j.rvsc.2018.11.006
2-s2.0-85056889750
url http://dx.doi.org/10.1016/j.rvsc.2018.11.006
http://hdl.handle.net/11449/188391
identifier_str_mv Research in Veterinary Science, v. 122, p. 86-92.
1532-2661
0034-5288
10.1016/j.rvsc.2018.11.006
2-s2.0-85056889750
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Research in Veterinary Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 86-92
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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