Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2019.106137 http://hdl.handle.net/11449/196375 |
Resumo: | To evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment. |
id |
UNSP_dfb4046c73cef0180e76a51c68ef376b |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/196375 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSHSuperovulationOvulatory follicular waveFollicular wall vascularizationApoptotic embryonic cellsOvineTo evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Agricultural Research CorporationPROPE / UnespSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFed Univ Jequitinhonha & Mucuri Valleys, Inst Agr Sci, Av Joao Narciso 1380, BR-38610000 Unai, MG, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilBrazilian Agr Res Corp Embrapa Dairy Cattle, BR-27640000 Valenca, RJ, BrazilUniv Fed Santa Maria, Cidade Univ, Dept Clin Large Anim, Av Roraima 1000,Cidade Univ, BR-97105900 Santa Maria, RS, BrazilBrazilian Agr Res Corp Embrapa Goats & Sheep, BR-36155000 Coronel Pacheco, MG, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2014/04614-6Brazilian Agricultural Research Corporation: 02.13.06.026.00.03PROPE / Unesp: TC1288 / 2015Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de Viçosa (UFV)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal de Sergipe (UFS)Maciel, Giovanna Serpa [UNESP]Kako Rodriguez, Mariana Garcia [UNESP]Correia Santos, Victor Jose [UNESP]Ramirez Uscategui, Ricardo AndresNociti, Ricardo Perecin [UNESP]Maronezi, Marjury Cristina [UNESP]Oliveira, Clara SladeRossi Feliciano, Marcus Antonio [UNESP]Russiano Vicente, Wilter Ricardo [UNESP]Fonseca, Jeferson Ferreira daFranco Oliveira, Maria Emilia [UNESP]2020-12-10T19:42:42Z2020-12-10T19:42:42Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.anireprosci.2019.106137Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019.0378-4320http://hdl.handle.net/11449/19637510.1016/j.anireprosci.2019.106137WOS:000500188100002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2024-06-06T18:09:35Zoai:repositorio.unesp.br:11449/196375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:05.422182Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
title |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
spellingShingle |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH Maciel, Giovanna Serpa [UNESP] Superovulation Ovulatory follicular wave Follicular wall vascularization Apoptotic embryonic cells Ovine |
title_short |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
title_full |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
title_fullStr |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
title_full_unstemmed |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
title_sort |
Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH |
author |
Maciel, Giovanna Serpa [UNESP] |
author_facet |
Maciel, Giovanna Serpa [UNESP] Kako Rodriguez, Mariana Garcia [UNESP] Correia Santos, Victor Jose [UNESP] Ramirez Uscategui, Ricardo Andres Nociti, Ricardo Perecin [UNESP] Maronezi, Marjury Cristina [UNESP] Oliveira, Clara Slade Rossi Feliciano, Marcus Antonio [UNESP] Russiano Vicente, Wilter Ricardo [UNESP] Fonseca, Jeferson Ferreira da Franco Oliveira, Maria Emilia [UNESP] |
author_role |
author |
author2 |
Kako Rodriguez, Mariana Garcia [UNESP] Correia Santos, Victor Jose [UNESP] Ramirez Uscategui, Ricardo Andres Nociti, Ricardo Perecin [UNESP] Maronezi, Marjury Cristina [UNESP] Oliveira, Clara Slade Rossi Feliciano, Marcus Antonio [UNESP] Russiano Vicente, Wilter Ricardo [UNESP] Fonseca, Jeferson Ferreira da Franco Oliveira, Maria Emilia [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Viçosa (UFV) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Federal de Sergipe (UFS) |
dc.contributor.author.fl_str_mv |
Maciel, Giovanna Serpa [UNESP] Kako Rodriguez, Mariana Garcia [UNESP] Correia Santos, Victor Jose [UNESP] Ramirez Uscategui, Ricardo Andres Nociti, Ricardo Perecin [UNESP] Maronezi, Marjury Cristina [UNESP] Oliveira, Clara Slade Rossi Feliciano, Marcus Antonio [UNESP] Russiano Vicente, Wilter Ricardo [UNESP] Fonseca, Jeferson Ferreira da Franco Oliveira, Maria Emilia [UNESP] |
dc.subject.por.fl_str_mv |
Superovulation Ovulatory follicular wave Follicular wall vascularization Apoptotic embryonic cells Ovine |
topic |
Superovulation Ovulatory follicular wave Follicular wall vascularization Apoptotic embryonic cells Ovine |
description |
To evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 2020-12-10T19:42:42Z 2020-12-10T19:42:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2019.106137 Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019. 0378-4320 http://hdl.handle.net/11449/196375 10.1016/j.anireprosci.2019.106137 WOS:000500188100002 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2019.106137 http://hdl.handle.net/11449/196375 |
identifier_str_mv |
Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019. 0378-4320 10.1016/j.anireprosci.2019.106137 WOS:000500188100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128959933579264 |