Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH

Detalhes bibliográficos
Autor(a) principal: Maciel, Giovanna Serpa [UNESP]
Data de Publicação: 2019
Outros Autores: Kako Rodriguez, Mariana Garcia [UNESP], Correia Santos, Victor Jose [UNESP], Ramirez Uscategui, Ricardo Andres, Nociti, Ricardo Perecin [UNESP], Maronezi, Marjury Cristina [UNESP], Oliveira, Clara Slade, Rossi Feliciano, Marcus Antonio [UNESP], Russiano Vicente, Wilter Ricardo [UNESP], Fonseca, Jeferson Ferreira da, Franco Oliveira, Maria Emilia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.anireprosci.2019.106137
http://hdl.handle.net/11449/196375
Resumo: To evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment.
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spelling Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSHSuperovulationOvulatory follicular waveFollicular wall vascularizationApoptotic embryonic cellsOvineTo evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Agricultural Research CorporationPROPE / UnespSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFed Univ Jequitinhonha & Mucuri Valleys, Inst Agr Sci, Av Joao Narciso 1380, BR-38610000 Unai, MG, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilBrazilian Agr Res Corp Embrapa Dairy Cattle, BR-27640000 Valenca, RJ, BrazilUniv Fed Santa Maria, Cidade Univ, Dept Clin Large Anim, Av Roraima 1000,Cidade Univ, BR-97105900 Santa Maria, RS, BrazilBrazilian Agr Res Corp Embrapa Goats & Sheep, BR-36155000 Coronel Pacheco, MG, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2014/04614-6Brazilian Agricultural Research Corporation: 02.13.06.026.00.03PROPE / Unesp: TC1288 / 2015Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de Viçosa (UFV)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal de Sergipe (UFS)Maciel, Giovanna Serpa [UNESP]Kako Rodriguez, Mariana Garcia [UNESP]Correia Santos, Victor Jose [UNESP]Ramirez Uscategui, Ricardo AndresNociti, Ricardo Perecin [UNESP]Maronezi, Marjury Cristina [UNESP]Oliveira, Clara SladeRossi Feliciano, Marcus Antonio [UNESP]Russiano Vicente, Wilter Ricardo [UNESP]Fonseca, Jeferson Ferreira daFranco Oliveira, Maria Emilia [UNESP]2020-12-10T19:42:42Z2020-12-10T19:42:42Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.1016/j.anireprosci.2019.106137Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019.0378-4320http://hdl.handle.net/11449/19637510.1016/j.anireprosci.2019.106137WOS:000500188100002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2024-06-06T18:09:35Zoai:repositorio.unesp.br:11449/196375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:05.422182Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
title Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
spellingShingle Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
Maciel, Giovanna Serpa [UNESP]
Superovulation
Ovulatory follicular wave
Follicular wall vascularization
Apoptotic embryonic cells
Ovine
title_short Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
title_full Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
title_fullStr Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
title_full_unstemmed Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
title_sort Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH
author Maciel, Giovanna Serpa [UNESP]
author_facet Maciel, Giovanna Serpa [UNESP]
Kako Rodriguez, Mariana Garcia [UNESP]
Correia Santos, Victor Jose [UNESP]
Ramirez Uscategui, Ricardo Andres
Nociti, Ricardo Perecin [UNESP]
Maronezi, Marjury Cristina [UNESP]
Oliveira, Clara Slade
Rossi Feliciano, Marcus Antonio [UNESP]
Russiano Vicente, Wilter Ricardo [UNESP]
Fonseca, Jeferson Ferreira da
Franco Oliveira, Maria Emilia [UNESP]
author_role author
author2 Kako Rodriguez, Mariana Garcia [UNESP]
Correia Santos, Victor Jose [UNESP]
Ramirez Uscategui, Ricardo Andres
Nociti, Ricardo Perecin [UNESP]
Maronezi, Marjury Cristina [UNESP]
Oliveira, Clara Slade
Rossi Feliciano, Marcus Antonio [UNESP]
Russiano Vicente, Wilter Ricardo [UNESP]
Fonseca, Jeferson Ferreira da
Franco Oliveira, Maria Emilia [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Viçosa (UFV)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal de Sergipe (UFS)
dc.contributor.author.fl_str_mv Maciel, Giovanna Serpa [UNESP]
Kako Rodriguez, Mariana Garcia [UNESP]
Correia Santos, Victor Jose [UNESP]
Ramirez Uscategui, Ricardo Andres
Nociti, Ricardo Perecin [UNESP]
Maronezi, Marjury Cristina [UNESP]
Oliveira, Clara Slade
Rossi Feliciano, Marcus Antonio [UNESP]
Russiano Vicente, Wilter Ricardo [UNESP]
Fonseca, Jeferson Ferreira da
Franco Oliveira, Maria Emilia [UNESP]
dc.subject.por.fl_str_mv Superovulation
Ovulatory follicular wave
Follicular wall vascularization
Apoptotic embryonic cells
Ovine
topic Superovulation
Ovulatory follicular wave
Follicular wall vascularization
Apoptotic embryonic cells
Ovine
description To evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-01
2020-12-10T19:42:42Z
2020-12-10T19:42:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.anireprosci.2019.106137
Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019.
0378-4320
http://hdl.handle.net/11449/196375
10.1016/j.anireprosci.2019.106137
WOS:000500188100002
url http://dx.doi.org/10.1016/j.anireprosci.2019.106137
http://hdl.handle.net/11449/196375
identifier_str_mv Animal Reproduction Science. Amsterdam: Elsevier, v. 209, 7 p., 2019.
0378-4320
10.1016/j.anireprosci.2019.106137
WOS:000500188100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Reproduction Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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