Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/161838 |
Resumo: | Larvae of honeybee workers were exposed to the insecticide fipronil during the feeding phase. To evaluate the effect of fipronil in the post-embryonic development of africanized Apis mellifera, bioassays of toxicity were done. The bioassays were performed by acute exposure applying 1 mu L of distilled water for control (I) and for experiments: 0.5 ng a.i./mu L of fipronil; 5 ng a.i./mu L of fipronil and 20 ng a.i./mu L. of fipronil. Triplicates were performed for all treatments. The results showed that the rate of anomalous pupae in exposed honeybees was statistically significant in relationship to the control (p <0:03). The most frequent abnormalities were: high pigmentation on the proximal and distal larval body and body malformation, such as absence of head and limbs. Pink eye pupa and white eyed pupae presented malformations in their larval bodies, but with the eye developed. It is assumed that the fat body is related to the high rate of anomalies, since this tissue has proteins linked to the process of metamorphosis. Furthermore, the fat body may be participating in the regulation of juvenile hormone during the process of metamorphosis, and consequently in the release of ecdysteroid hormones that are involved in the change from larva to adult. The high rate of abnormalities in the pupal stage of individuals exposed to fipronil raises concerns about the impacts caused in the colonies of bees and population decline of pollinators. |
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Fipronil effect on the frequency of anomalous brood in honeybee reared in vitrobeeslarvaepupaemetamorphosisanomaliesfipronilLarvae of honeybee workers were exposed to the insecticide fipronil during the feeding phase. To evaluate the effect of fipronil in the post-embryonic development of africanized Apis mellifera, bioassays of toxicity were done. The bioassays were performed by acute exposure applying 1 mu L of distilled water for control (I) and for experiments: 0.5 ng a.i./mu L of fipronil; 5 ng a.i./mu L of fipronil and 20 ng a.i./mu L. of fipronil. Triplicates were performed for all treatments. The results showed that the rate of anomalous pupae in exposed honeybees was statistically significant in relationship to the control (p <0:03). The most frequent abnormalities were: high pigmentation on the proximal and distal larval body and body malformation, such as absence of head and limbs. Pink eye pupa and white eyed pupae presented malformations in their larval bodies, but with the eye developed. It is assumed that the fat body is related to the high rate of anomalies, since this tissue has proteins linked to the process of metamorphosis. Furthermore, the fat body may be participating in the regulation of juvenile hormone during the process of metamorphosis, and consequently in the release of ecdysteroid hormones that are involved in the change from larva to adult. The high rate of abnormalities in the pupal stage of individuals exposed to fipronil raises concerns about the impacts caused in the colonies of bees and population decline of pollinators.UFSCar Univ Fed Sao Carlos, CCA, Rod Anhanguera,SP 330,Km 174, Araras, SP, BrazilUniv Fed Sao Carlos UFSCar, LABEF Lab Biol Estrutural & Func, Sorocaba, SP, BrazilUniv Estadual Julio de Mesquita Filho UNESP, CEIS, Rio Claro, SP, BrazilUniv Estadual Julio de Mesquita Filho UNESP, CEIS, Rio Claro, SP, BrazilJulius Kuhn-instUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Silva, Carina A. S.Silva-Zacarin, Elaine C. M.Domingues, Caio E. C.Abdalla, Fabio C.Malaspina, Osmar [UNESP]Nocelli, Roberta C. F.Oomen, P. A.Pistorius, J.2018-11-26T16:56:26Z2018-11-26T16:56:26Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject140-146application/pdfHazards Of Pesticides To Bees: 12th International Symposium Of The Icp-pr Bee Protection Group. Berlin: Julius Kuhn-inst, v. 450, p. 140-146, 2015.1868-9892http://hdl.handle.net/11449/161838WOS:000381782400022WOS000381782400022.pdf75385560855058190000-0002-1650-257XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHazards Of Pesticides To Bees: 12th International Symposium Of The Icp-pr Bee Protection Groupinfo:eu-repo/semantics/openAccess2024-04-15T19:03:57Zoai:repositorio.unesp.br:11449/161838Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:23:41.036103Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
title |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
spellingShingle |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro Silva, Carina A. S. bees larvae pupae metamorphosis anomalies fipronil |
title_short |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
title_full |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
title_fullStr |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
title_full_unstemmed |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
title_sort |
Fipronil effect on the frequency of anomalous brood in honeybee reared in vitro |
author |
Silva, Carina A. S. |
author_facet |
Silva, Carina A. S. Silva-Zacarin, Elaine C. M. Domingues, Caio E. C. Abdalla, Fabio C. Malaspina, Osmar [UNESP] Nocelli, Roberta C. F. Oomen, P. A. Pistorius, J. |
author_role |
author |
author2 |
Silva-Zacarin, Elaine C. M. Domingues, Caio E. C. Abdalla, Fabio C. Malaspina, Osmar [UNESP] Nocelli, Roberta C. F. Oomen, P. A. Pistorius, J. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Carina A. S. Silva-Zacarin, Elaine C. M. Domingues, Caio E. C. Abdalla, Fabio C. Malaspina, Osmar [UNESP] Nocelli, Roberta C. F. Oomen, P. A. Pistorius, J. |
dc.subject.por.fl_str_mv |
bees larvae pupae metamorphosis anomalies fipronil |
topic |
bees larvae pupae metamorphosis anomalies fipronil |
description |
Larvae of honeybee workers were exposed to the insecticide fipronil during the feeding phase. To evaluate the effect of fipronil in the post-embryonic development of africanized Apis mellifera, bioassays of toxicity were done. The bioassays were performed by acute exposure applying 1 mu L of distilled water for control (I) and for experiments: 0.5 ng a.i./mu L of fipronil; 5 ng a.i./mu L of fipronil and 20 ng a.i./mu L. of fipronil. Triplicates were performed for all treatments. The results showed that the rate of anomalous pupae in exposed honeybees was statistically significant in relationship to the control (p <0:03). The most frequent abnormalities were: high pigmentation on the proximal and distal larval body and body malformation, such as absence of head and limbs. Pink eye pupa and white eyed pupae presented malformations in their larval bodies, but with the eye developed. It is assumed that the fat body is related to the high rate of anomalies, since this tissue has proteins linked to the process of metamorphosis. Furthermore, the fat body may be participating in the regulation of juvenile hormone during the process of metamorphosis, and consequently in the release of ecdysteroid hormones that are involved in the change from larva to adult. The high rate of abnormalities in the pupal stage of individuals exposed to fipronil raises concerns about the impacts caused in the colonies of bees and population decline of pollinators. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-11-26T16:56:26Z 2018-11-26T16:56:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Hazards Of Pesticides To Bees: 12th International Symposium Of The Icp-pr Bee Protection Group. Berlin: Julius Kuhn-inst, v. 450, p. 140-146, 2015. 1868-9892 http://hdl.handle.net/11449/161838 WOS:000381782400022 WOS000381782400022.pdf 7538556085505819 0000-0002-1650-257X |
identifier_str_mv |
Hazards Of Pesticides To Bees: 12th International Symposium Of The Icp-pr Bee Protection Group. Berlin: Julius Kuhn-inst, v. 450, p. 140-146, 2015. 1868-9892 WOS:000381782400022 WOS000381782400022.pdf 7538556085505819 0000-0002-1650-257X |
url |
http://hdl.handle.net/11449/161838 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hazards Of Pesticides To Bees: 12th International Symposium Of The Icp-pr Bee Protection Group |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
140-146 application/pdf |
dc.publisher.none.fl_str_mv |
Julius Kuhn-inst |
publisher.none.fl_str_mv |
Julius Kuhn-inst |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129197273513984 |