Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates

Detalhes bibliográficos
Autor(a) principal: Okura, Mõnica Hitomi
Data de Publicação: 2014
Outros Autores: Marin, José Moacir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20131237
http://hdl.handle.net/11449/114262
Resumo: The soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells. The only gene detected by PCR was the sfa gene at one isolate. The strains were also screened for resistance to 9 antimicrobial drugs. Predominant resistance was to cephalothin, tetracycline and streptomycin. Multidrug resistance was found among isolates from cheese with SIF (16.6%), cheese without SIF (8.0%) and cheese without SIF with spice added (30.0%) what is a reason for concern due to the high consumption of raw milk cheese by the Brazilian population.
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spelling Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolatesAvaliação em queijos Minas frescal provenientes do Sudoeste de Minas Gerais da presença de fatores de virulência e resistência antimicrobiana em isolados de Escherichia coliEscherichia coliqueijoSTECExPECresistência antimicrobianaMinas frescalEscherichia colicheeseSTECExPECantimicrobial resistanceMinas frescalThe soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells. The only gene detected by PCR was the sfa gene at one isolate. The strains were also screened for resistance to 9 antimicrobial drugs. Predominant resistance was to cephalothin, tetracycline and streptomycin. Multidrug resistance was found among isolates from cheese with SIF (16.6%), cheese without SIF (8.0%) and cheese without SIF with spice added (30.0%) what is a reason for concern due to the high consumption of raw milk cheese by the Brazilian population.O queijo Minas frescal é um dos queijos mais populares no Brasil, ele é tipicamente manufaturado em pequenas fazendas de leite, em condições higiênicas insatisfatórias. Para verificar o risco envolvido no consumo deste queijo, marcadores de virulência foram investigados em 330 cepas de isoladas de 30 queijos Minas frescal, inspecionados pela agencia governamental oficial (SIF- serviço de inspeção federal), de 50 queijos não inspecionados pelo SIF e de 31 queijos não inspecionados pelo SIF e com condimentos adicionados. Todos eles foram coletados na região sudeste do Estado de Minas Gerais. As cepas de E. coli foram examinadas para a presença de genes codificadores de Shiga toxina (stx1 e stx 2), gene da intimina (eae) e também para a presença dos genes (pap, sfa, afa) relacionados com a adesão em células epiteliais. O único gene detectado por PCR foi sfa em um isolado. As cepas também foram examinadas para a resistência a nove fármacos antimicrobianos. As resistências predominantemente detectadas foram para cefalotina, tetraciclina e estreptomicina. A resistência a múltiplas drogas identificadas entre as cepas de E. coli, provenientes de queijo com SIF (16,6%), queijo sem SIF (8,0%) e queijo sem SIF com condimentos adicionados (30,0%), o que representa um motivo de preocupação, devido ao elevado consumo pela população Brasileira de queijos feitos de leite não pasterurizado.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias de JaboticabalUniversidade de São Paulo Faculdade de Odontologia de Ribeirão Preto Fisiologia e Patologia BásicaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias de JaboticabalUniversidade Federal de Santa MariaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Okura, Mõnica HitomiMarin, José Moacir2015-02-02T12:39:23Z2015-02-02T12:39:23Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1506-1511application/pdfhttp://dx.doi.org/10.1590/0103-8478cr20131237Ciência Rural. Universidade Federal de Santa Maria, v. 44, n. 8, p. 1506-1511, 2014.0103-8478http://hdl.handle.net/11449/11426210.1590/0103-8478cr20131237S0103-84782014000801506S0103-84782014000801506.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiência Rural0.5250,337info:eu-repo/semantics/openAccess2023-10-24T06:06:46Zoai:repositorio.unesp.br:11449/114262Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-24T06:06:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
Avaliação em queijos Minas frescal provenientes do Sudoeste de Minas Gerais da presença de fatores de virulência e resistência antimicrobiana em isolados de Escherichia coli
title Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
spellingShingle Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
Okura, Mõnica Hitomi
Escherichia coli
queijo
STEC
ExPEC
resistência antimicrobiana
Minas frescal
Escherichia coli
cheese
STEC
ExPEC
antimicrobial resistance
Minas frescal
title_short Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
title_full Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
title_fullStr Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
title_full_unstemmed Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
title_sort Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
author Okura, Mõnica Hitomi
author_facet Okura, Mõnica Hitomi
Marin, José Moacir
author_role author
author2 Marin, José Moacir
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Okura, Mõnica Hitomi
Marin, José Moacir
dc.subject.por.fl_str_mv Escherichia coli
queijo
STEC
ExPEC
resistência antimicrobiana
Minas frescal
Escherichia coli
cheese
STEC
ExPEC
antimicrobial resistance
Minas frescal
topic Escherichia coli
queijo
STEC
ExPEC
resistência antimicrobiana
Minas frescal
Escherichia coli
cheese
STEC
ExPEC
antimicrobial resistance
Minas frescal
description The soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells. The only gene detected by PCR was the sfa gene at one isolate. The strains were also screened for resistance to 9 antimicrobial drugs. Predominant resistance was to cephalothin, tetracycline and streptomycin. Multidrug resistance was found among isolates from cheese with SIF (16.6%), cheese without SIF (8.0%) and cheese without SIF with spice added (30.0%) what is a reason for concern due to the high consumption of raw milk cheese by the Brazilian population.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-02-02T12:39:23Z
2015-02-02T12:39:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20131237
Ciência Rural. Universidade Federal de Santa Maria, v. 44, n. 8, p. 1506-1511, 2014.
0103-8478
http://hdl.handle.net/11449/114262
10.1590/0103-8478cr20131237
S0103-84782014000801506
S0103-84782014000801506.pdf
url http://dx.doi.org/10.1590/0103-8478cr20131237
http://hdl.handle.net/11449/114262
identifier_str_mv Ciência Rural. Universidade Federal de Santa Maria, v. 44, n. 8, p. 1506-1511, 2014.
0103-8478
10.1590/0103-8478cr20131237
S0103-84782014000801506
S0103-84782014000801506.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciência Rural
0.525
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1506-1511
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797789493709766656

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