Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/zsc.12469 http://hdl.handle.net/11449/209787 |
Resumo: | Hemiodus is the largest genus of the South American freshwater fish family Hemiodontidae, with 23 valid species. However, the species-level diversity of Hemiodus remains uncertain since morphological studies have indicated additional species. Also, the phylogenetic relationships among species and species boundaries within this genus are still unresolved. Herein, we sequenced the barcode (COI) gene of 19 species of Hemiodus on which undertook molecular species delimitation methods (automatic barcode gap discovery [ABGD], generalized mixed Yule coalescent [GMYC] and Poisson tree process [PTP]) and the standard DNA barcoding procedure to establish species boundaries and cast light on putative new species in the genus. We also provide a COI-based phylogenetic hypothesis for Hemiodus by maximum-likelihood (ML) analysis, the first at species level. The results of ABGD, GMYC and PTP indicate that around 30 MOTUs (range: 28-32) exist for Hemiodus within our data set of 19 valid species with concordance among strategies (i.e. equal results) in delimiting 24 MOTUs. Using classical DNA barcoding procedures, we settled on 28 MOTUs for Hemiodus. Undescribed morphospecies (i.e. feasible for description or taxonomic validation), as well as cryptic species (i.e. lineages with high genetic distance but indistinguishable in external morphology), are proposed. Even with the limitations of a single-locus phylogeny, the ML tree recovered many species and species groups as clades, corroborating, thus, their taxonomic separation. Overall, our results highlight the existence of an overlooked diversity of mitochondrial lineages in Hemiodus that can represent cryptic or poorly scrutinized species. Moreover, this study provides a molecular backbone for future studies on the systematics and evolution of Hemiodontidae taxa. |
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Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes)DNA barcodingfreshwater fishesHemiodontidaeSouth AmericaHemiodus is the largest genus of the South American freshwater fish family Hemiodontidae, with 23 valid species. However, the species-level diversity of Hemiodus remains uncertain since morphological studies have indicated additional species. Also, the phylogenetic relationships among species and species boundaries within this genus are still unresolved. Herein, we sequenced the barcode (COI) gene of 19 species of Hemiodus on which undertook molecular species delimitation methods (automatic barcode gap discovery [ABGD], generalized mixed Yule coalescent [GMYC] and Poisson tree process [PTP]) and the standard DNA barcoding procedure to establish species boundaries and cast light on putative new species in the genus. We also provide a COI-based phylogenetic hypothesis for Hemiodus by maximum-likelihood (ML) analysis, the first at species level. The results of ABGD, GMYC and PTP indicate that around 30 MOTUs (range: 28-32) exist for Hemiodus within our data set of 19 valid species with concordance among strategies (i.e. equal results) in delimiting 24 MOTUs. Using classical DNA barcoding procedures, we settled on 28 MOTUs for Hemiodus. Undescribed morphospecies (i.e. feasible for description or taxonomic validation), as well as cryptic species (i.e. lineages with high genetic distance but indistinguishable in external morphology), are proposed. Even with the limitations of a single-locus phylogeny, the ML tree recovered many species and species groups as clades, corroborating, thus, their taxonomic separation. Overall, our results highlight the existence of an overlooked diversity of mitochondrial lineages in Hemiodus that can represent cryptic or poorly scrutinized species. Moreover, this study provides a molecular backbone for future studies on the systematics and evolution of Hemiodontidae taxa.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Para, Inst Ciencias Biol, Programa Posgrad Zool, Belem, Para, BrazilMuseu Paraense Emilio Goeldi, Belem, Para, BrazilUniv Estadual Paulista, Lab Biol & Genet Peixes, Dept Biol Estrutural & Func, Inst Biociencias, R Prof Dr Antonio CW Zanin,250, BR-18618689 Botucatu, SP, BrazilUniv Fed Rio Grande do Sul, Inst Biociencias, Dept Zool, Lab Ictiol, Porto Alegre, RS, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Zool & Bot, Sao Jose Do Rio Preto, BrazilUniv Estadual Paulista, Lab Biol & Genet Peixes, Dept Biol Estrutural & Func, Inst Biociencias, R Prof Dr Antonio CW Zanin,250, BR-18618689 Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Zool & Bot, Sao Jose Do Rio Preto, BrazilWiley-BlackwellUniv Fed ParaMuseu Paraense Emilio GoeldiUniversidade Estadual Paulista (Unesp)Univ Fed Rio Grande do SulNogueira, Acacio F. [UNESP]Oliveira, Claudio [UNESP]Langeani, Francisco [UNESP]Netto-Ferreira, Andre L.2021-06-25T12:29:18Z2021-06-25T12:29:18Z2020-12-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article337-351http://dx.doi.org/10.1111/zsc.12469Zoologica Scripta. Hoboken: Wiley, v. 50, n. 3, p. 337-351, 2021.0300-3256http://hdl.handle.net/11449/20978710.1111/zsc.12469WOS:000600012300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZoologica Scriptainfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209787Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:57:09.666929Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
title |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
spellingShingle |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) Nogueira, Acacio F. [UNESP] DNA barcoding freshwater fishes Hemiodontidae South America |
title_short |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
title_full |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
title_fullStr |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
title_full_unstemmed |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
title_sort |
Overlooked biodiversity of mitochondrial lineages in Hemiodus (Ostariophysi, Characiformes) |
author |
Nogueira, Acacio F. [UNESP] |
author_facet |
Nogueira, Acacio F. [UNESP] Oliveira, Claudio [UNESP] Langeani, Francisco [UNESP] Netto-Ferreira, Andre L. |
author_role |
author |
author2 |
Oliveira, Claudio [UNESP] Langeani, Francisco [UNESP] Netto-Ferreira, Andre L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Para Museu Paraense Emilio Goeldi Universidade Estadual Paulista (Unesp) Univ Fed Rio Grande do Sul |
dc.contributor.author.fl_str_mv |
Nogueira, Acacio F. [UNESP] Oliveira, Claudio [UNESP] Langeani, Francisco [UNESP] Netto-Ferreira, Andre L. |
dc.subject.por.fl_str_mv |
DNA barcoding freshwater fishes Hemiodontidae South America |
topic |
DNA barcoding freshwater fishes Hemiodontidae South America |
description |
Hemiodus is the largest genus of the South American freshwater fish family Hemiodontidae, with 23 valid species. However, the species-level diversity of Hemiodus remains uncertain since morphological studies have indicated additional species. Also, the phylogenetic relationships among species and species boundaries within this genus are still unresolved. Herein, we sequenced the barcode (COI) gene of 19 species of Hemiodus on which undertook molecular species delimitation methods (automatic barcode gap discovery [ABGD], generalized mixed Yule coalescent [GMYC] and Poisson tree process [PTP]) and the standard DNA barcoding procedure to establish species boundaries and cast light on putative new species in the genus. We also provide a COI-based phylogenetic hypothesis for Hemiodus by maximum-likelihood (ML) analysis, the first at species level. The results of ABGD, GMYC and PTP indicate that around 30 MOTUs (range: 28-32) exist for Hemiodus within our data set of 19 valid species with concordance among strategies (i.e. equal results) in delimiting 24 MOTUs. Using classical DNA barcoding procedures, we settled on 28 MOTUs for Hemiodus. Undescribed morphospecies (i.e. feasible for description or taxonomic validation), as well as cryptic species (i.e. lineages with high genetic distance but indistinguishable in external morphology), are proposed. Even with the limitations of a single-locus phylogeny, the ML tree recovered many species and species groups as clades, corroborating, thus, their taxonomic separation. Overall, our results highlight the existence of an overlooked diversity of mitochondrial lineages in Hemiodus that can represent cryptic or poorly scrutinized species. Moreover, this study provides a molecular backbone for future studies on the systematics and evolution of Hemiodontidae taxa. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-19 2021-06-25T12:29:18Z 2021-06-25T12:29:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/zsc.12469 Zoologica Scripta. Hoboken: Wiley, v. 50, n. 3, p. 337-351, 2021. 0300-3256 http://hdl.handle.net/11449/209787 10.1111/zsc.12469 WOS:000600012300001 |
url |
http://dx.doi.org/10.1111/zsc.12469 http://hdl.handle.net/11449/209787 |
identifier_str_mv |
Zoologica Scripta. Hoboken: Wiley, v. 50, n. 3, p. 337-351, 2021. 0300-3256 10.1111/zsc.12469 WOS:000600012300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zoologica Scripta |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
337-351 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129266460655616 |