Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4322/bds.2023.e3672 http://hdl.handle.net/11449/248493 |
Resumo: | Objective: to investigate the antimicrobial effects of toothpastes containing bioactive surface pre-reacted glass particles (S-PRG) on S. mutans biofilms adherence, initial colonization and maturation. Material and Methods: a reference UA 159 and a clinical S. mutans (SM6) strain were used. Bovine enamel specimens were randomly allocated into the groups (n=5): toothpastes containing 0%; 1%; 5%; 20%; 30% S-PRG; positive control dentifrice (NaF+triclosan); and negative control (distilled water). For biofilm development, samples were placed in a 24-well plate containing artificial saliva (4h), followed by adding 1mL of artificial saliva, BHI broth and 225μL of S. mutans suspension. Treatments with toothpastes were applied previously or after 4h and 24h of biofilm formation. Samples were incubated for 48h at 37°C in 5%CO2 and biofilm was detached and seeded in Petri dishes for determining the number of viable cells. Data were analyzed by ANOVA and Tukey test (5%). Results: significantly lower microorganisms’ adherence (p<0.05) was obtained for all S-PRG toothpastes, with similar results to NaF+triclosan for SM6 and 20 and 30%S-PRG groups exhibiting higher inhibition effect than the NaF+Triclosan for UA159. Antibacterial effect on the early-stage biofilm was also observed for the S-PRG groups, but was not superior to the NaF+Triclosan toothpaste. For the mature biofilm, the effective antimicrobial potential of S-PRG toothpastes was observed only for the SM6 clinical strain, but was not higher than the positive control. Conclusion: experimental S-PRG toothpastes were effective to inhibit S. mutans biofilm growth by exhibiting antimicrobial activity, being promising agents to prevent cariogenic biofilm development. |
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Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm developmentEficácia antimicrobiana de dentifrícios contendo S-PRG sobre o desenvolvimento de biofilme de S. mutansBiofilmDental enamelGiomerStreptococcus mutans, S-PRGObjective: to investigate the antimicrobial effects of toothpastes containing bioactive surface pre-reacted glass particles (S-PRG) on S. mutans biofilms adherence, initial colonization and maturation. Material and Methods: a reference UA 159 and a clinical S. mutans (SM6) strain were used. Bovine enamel specimens were randomly allocated into the groups (n=5): toothpastes containing 0%; 1%; 5%; 20%; 30% S-PRG; positive control dentifrice (NaF+triclosan); and negative control (distilled water). For biofilm development, samples were placed in a 24-well plate containing artificial saliva (4h), followed by adding 1mL of artificial saliva, BHI broth and 225μL of S. mutans suspension. Treatments with toothpastes were applied previously or after 4h and 24h of biofilm formation. Samples were incubated for 48h at 37°C in 5%CO2 and biofilm was detached and seeded in Petri dishes for determining the number of viable cells. Data were analyzed by ANOVA and Tukey test (5%). Results: significantly lower microorganisms’ adherence (p<0.05) was obtained for all S-PRG toothpastes, with similar results to NaF+triclosan for SM6 and 20 and 30%S-PRG groups exhibiting higher inhibition effect than the NaF+Triclosan for UA159. Antibacterial effect on the early-stage biofilm was also observed for the S-PRG groups, but was not superior to the NaF+Triclosan toothpaste. For the mature biofilm, the effective antimicrobial potential of S-PRG toothpastes was observed only for the SM6 clinical strain, but was not higher than the positive control. Conclusion: experimental S-PRG toothpastes were effective to inhibit S. mutans biofilm growth by exhibiting antimicrobial activity, being promising agents to prevent cariogenic biofilm development.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Odontologia da Universidade Braz CubasInstituto de Ciência e Tecnologia da UNESP Departamento de Odontologia RestauradoraInstituto de Ciência e Tecnologia da UNESP Departamento de Biociências e Diagnóstico OralInstituto de Ciência e Tecnologia da UNESP Departamento de Odontologia RestauradoraInstituto de Ciência e Tecnologia da UNESP Departamento de Biociências e Diagnóstico OralCNPq: 51256/2019Faculdade de Odontologia da Universidade Braz CubasUniversidade Estadual Paulista (UNESP)Spinola, Manuela da SilvaMendonça, Jacqueline Landi [UNESP]Garcia, Maíra Terra [UNESP]Caneppele, Taciana Marco Ferraz [UNESP]Junqueira, Juliana Campos [UNESP]Torres, Carlos Rocha Gomes [UNESP]Borges, Alessandra Buhler [UNESP]2023-07-29T13:45:29Z2023-07-29T13:45:29Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4322/bds.2023.e3672Brazilian Dental Science, v. 26, n. 1, 2023.2178-6011http://hdl.handle.net/11449/24849310.4322/bds.2023.e36722-s2.0-85149767498Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Scienceinfo:eu-repo/semantics/openAccess2023-07-29T13:45:29Zoai:repositorio.unesp.br:11449/248493Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:45:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development Eficácia antimicrobiana de dentifrícios contendo S-PRG sobre o desenvolvimento de biofilme de S. mutans |
title |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
spellingShingle |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development Spinola, Manuela da Silva Biofilm Dental enamel Giomer Streptococcus mutans, S-PRG |
title_short |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
title_full |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
title_fullStr |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
title_full_unstemmed |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
title_sort |
Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development |
author |
Spinola, Manuela da Silva |
author_facet |
Spinola, Manuela da Silva Mendonça, Jacqueline Landi [UNESP] Garcia, Maíra Terra [UNESP] Caneppele, Taciana Marco Ferraz [UNESP] Junqueira, Juliana Campos [UNESP] Torres, Carlos Rocha Gomes [UNESP] Borges, Alessandra Buhler [UNESP] |
author_role |
author |
author2 |
Mendonça, Jacqueline Landi [UNESP] Garcia, Maíra Terra [UNESP] Caneppele, Taciana Marco Ferraz [UNESP] Junqueira, Juliana Campos [UNESP] Torres, Carlos Rocha Gomes [UNESP] Borges, Alessandra Buhler [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Odontologia da Universidade Braz Cubas Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Spinola, Manuela da Silva Mendonça, Jacqueline Landi [UNESP] Garcia, Maíra Terra [UNESP] Caneppele, Taciana Marco Ferraz [UNESP] Junqueira, Juliana Campos [UNESP] Torres, Carlos Rocha Gomes [UNESP] Borges, Alessandra Buhler [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Dental enamel Giomer Streptococcus mutans, S-PRG |
topic |
Biofilm Dental enamel Giomer Streptococcus mutans, S-PRG |
description |
Objective: to investigate the antimicrobial effects of toothpastes containing bioactive surface pre-reacted glass particles (S-PRG) on S. mutans biofilms adherence, initial colonization and maturation. Material and Methods: a reference UA 159 and a clinical S. mutans (SM6) strain were used. Bovine enamel specimens were randomly allocated into the groups (n=5): toothpastes containing 0%; 1%; 5%; 20%; 30% S-PRG; positive control dentifrice (NaF+triclosan); and negative control (distilled water). For biofilm development, samples were placed in a 24-well plate containing artificial saliva (4h), followed by adding 1mL of artificial saliva, BHI broth and 225μL of S. mutans suspension. Treatments with toothpastes were applied previously or after 4h and 24h of biofilm formation. Samples were incubated for 48h at 37°C in 5%CO2 and biofilm was detached and seeded in Petri dishes for determining the number of viable cells. Data were analyzed by ANOVA and Tukey test (5%). Results: significantly lower microorganisms’ adherence (p<0.05) was obtained for all S-PRG toothpastes, with similar results to NaF+triclosan for SM6 and 20 and 30%S-PRG groups exhibiting higher inhibition effect than the NaF+Triclosan for UA159. Antibacterial effect on the early-stage biofilm was also observed for the S-PRG groups, but was not superior to the NaF+Triclosan toothpaste. For the mature biofilm, the effective antimicrobial potential of S-PRG toothpastes was observed only for the SM6 clinical strain, but was not higher than the positive control. Conclusion: experimental S-PRG toothpastes were effective to inhibit S. mutans biofilm growth by exhibiting antimicrobial activity, being promising agents to prevent cariogenic biofilm development. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:45:29Z 2023-07-29T13:45:29Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4322/bds.2023.e3672 Brazilian Dental Science, v. 26, n. 1, 2023. 2178-6011 http://hdl.handle.net/11449/248493 10.4322/bds.2023.e3672 2-s2.0-85149767498 |
url |
http://dx.doi.org/10.4322/bds.2023.e3672 http://hdl.handle.net/11449/248493 |
identifier_str_mv |
Brazilian Dental Science, v. 26, n. 1, 2023. 2178-6011 10.4322/bds.2023.e3672 2-s2.0-85149767498 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964524268224512 |