Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-020-03612-x http://hdl.handle.net/11449/208036 |
Resumo: | Objectives: The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods: Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, ± 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results: GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion: PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. Clinical relevance: The PEMF therapy is an effective alternative method for optimizing bone healing. |
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Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro studyBone-anchored implantsElectromagnetic fieldsOsseointegrationRatsObjectives: The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods: Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, ± 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results: GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion: PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. Clinical relevance: The PEMF therapy is an effective alternative method for optimizing bone healing.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos, Av Engenheiro Francisco José Longo, 777, São DimasDepartment of Bioscience and Bucal Diagnosis São Paulo State University (UNESP) Institute of Science and Technology of São José dos CamposDepartment of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology of São José dos Campos, Av Engenheiro Francisco José Longo, 777, São DimasDepartment of Bioscience and Bucal Diagnosis São Paulo State University (UNESP) Institute of Science and Technology of São José dos CamposCAPES: 001FAPESP: 2018/13942-8FAPESP: 2019/01669-8CNPq: CNPq – 465259/2014-6Universidade Estadual Paulista (Unesp)Nunes, Camilla Magnoni Moretto [UNESP]Ferreira, Camila Lopes [UNESP]Bernardo, Daniella Vicensotto [UNESP]Lopes, Cássia Carolina Rabelo [UNESP]Collino, Luma [UNESP]da Silva Lima, Victória Clara [UNESP]de Camargo Reis Mello, Daphne [UNESP]de Vasconcellos, Luana Marotta Reis [UNESP]Jardini, Maria Aparecida Neves [UNESP]2021-06-25T11:05:18Z2021-06-25T11:05:18Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2925-2937http://dx.doi.org/10.1007/s00784-020-03612-xClinical Oral Investigations, v. 25, n. 5, p. 2925-2937, 2021.1436-37711432-6981http://hdl.handle.net/11449/20803610.1007/s00784-020-03612-x2-s2.0-85092380811Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-10-23T18:51:59Zoai:repositorio.unesp.br:11449/208036Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:31:22.613646Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
title |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
spellingShingle |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study Nunes, Camilla Magnoni Moretto [UNESP] Bone-anchored implants Electromagnetic fields Osseointegration Rats |
title_short |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
title_full |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
title_fullStr |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
title_full_unstemmed |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
title_sort |
Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study |
author |
Nunes, Camilla Magnoni Moretto [UNESP] |
author_facet |
Nunes, Camilla Magnoni Moretto [UNESP] Ferreira, Camila Lopes [UNESP] Bernardo, Daniella Vicensotto [UNESP] Lopes, Cássia Carolina Rabelo [UNESP] Collino, Luma [UNESP] da Silva Lima, Victória Clara [UNESP] de Camargo Reis Mello, Daphne [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Jardini, Maria Aparecida Neves [UNESP] |
author_role |
author |
author2 |
Ferreira, Camila Lopes [UNESP] Bernardo, Daniella Vicensotto [UNESP] Lopes, Cássia Carolina Rabelo [UNESP] Collino, Luma [UNESP] da Silva Lima, Victória Clara [UNESP] de Camargo Reis Mello, Daphne [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Jardini, Maria Aparecida Neves [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nunes, Camilla Magnoni Moretto [UNESP] Ferreira, Camila Lopes [UNESP] Bernardo, Daniella Vicensotto [UNESP] Lopes, Cássia Carolina Rabelo [UNESP] Collino, Luma [UNESP] da Silva Lima, Victória Clara [UNESP] de Camargo Reis Mello, Daphne [UNESP] de Vasconcellos, Luana Marotta Reis [UNESP] Jardini, Maria Aparecida Neves [UNESP] |
dc.subject.por.fl_str_mv |
Bone-anchored implants Electromagnetic fields Osseointegration Rats |
topic |
Bone-anchored implants Electromagnetic fields Osseointegration Rats |
description |
Objectives: The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods: Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, ± 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results: GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion: PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. Clinical relevance: The PEMF therapy is an effective alternative method for optimizing bone healing. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:05:18Z 2021-06-25T11:05:18Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-020-03612-x Clinical Oral Investigations, v. 25, n. 5, p. 2925-2937, 2021. 1436-3771 1432-6981 http://hdl.handle.net/11449/208036 10.1007/s00784-020-03612-x 2-s2.0-85092380811 |
url |
http://dx.doi.org/10.1007/s00784-020-03612-x http://hdl.handle.net/11449/208036 |
identifier_str_mv |
Clinical Oral Investigations, v. 25, n. 5, p. 2925-2937, 2021. 1436-3771 1432-6981 10.1007/s00784-020-03612-x 2-s2.0-85092380811 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2925-2937 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128942287093760 |