Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material

Detalhes bibliográficos
Autor(a) principal: Okamoto, AS [UNESP]
Data de Publicação: 2009
Outros Autores: Andreatti Filho, RL [UNESP], Rocha, TS [UNESP], Menconi, A [UNESP], Marietto-Gonçalves, GA [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-635X2009000300009
http://hdl.handle.net/11449/13692
Resumo: The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples were evaluated for their capacity to horizontally transfer this gene. There was no direct relationship between the presence of the class 1 integron gene and SE resistance to the 14 antimicrobials tested, as 80% of the studied samples were resistant to up to three antimicrobials, and did not present the aforementioned gene. However, horizontal transfer of this gene was accomplished in vitro (from Escherichia coli to Salmonella Enteritidis), demonstrating that capacity class 1 integron gene can be disseminated among enterobacteria.
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spelling Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian materialIntegrongene transferresistance to antimicrobialsSalmonella EnteritidisThe expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples were evaluated for their capacity to horizontally transfer this gene. There was no direct relationship between the presence of the class 1 integron gene and SE resistance to the 14 antimicrobials tested, as 80% of the studied samples were resistant to up to three antimicrobials, and did not present the aforementioned gene. However, horizontal transfer of this gene was accomplished in vitro (from Escherichia coli to Salmonella Enteritidis), demonstrating that capacity class 1 integron gene can be disseminated among enterobacteria.UNESP FMVZ Department of Veterinary ClinicsUNESP FMVZ Ornithopathology serviceUNESP FMVZ Department of Veterinary ClinicsUNESP FMVZ Ornithopathology serviceFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Okamoto, AS [UNESP]Andreatti Filho, RL [UNESP]Rocha, TS [UNESP]Menconi, A [UNESP]Marietto-Gonçalves, GA [UNESP]2014-05-20T13:39:29Z2014-05-20T13:39:29Z2009-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-201application/pdfhttp://dx.doi.org/10.1590/S1516-635X2009000300009Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 3, p. 195-201, 2009.1516-635Xhttp://hdl.handle.net/11449/1369210.1590/S1516-635X2009000300009S1516-635X2009000300009WOS:000272254000009S1516-635X2009000300009.pdf8502462873517464SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2023-10-23T06:06:04Zoai:repositorio.unesp.br:11449/13692Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-23T06:06:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
title Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
spellingShingle Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
Okamoto, AS [UNESP]
Integron
gene transfer
resistance to antimicrobials
Salmonella Enteritidis
title_short Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
title_full Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
title_fullStr Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
title_full_unstemmed Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
title_sort Detection and transfer of antimicrobial resistance gene integron in Salmonella Enteritidis derived from avian material
author Okamoto, AS [UNESP]
author_facet Okamoto, AS [UNESP]
Andreatti Filho, RL [UNESP]
Rocha, TS [UNESP]
Menconi, A [UNESP]
Marietto-Gonçalves, GA [UNESP]
author_role author
author2 Andreatti Filho, RL [UNESP]
Rocha, TS [UNESP]
Menconi, A [UNESP]
Marietto-Gonçalves, GA [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Okamoto, AS [UNESP]
Andreatti Filho, RL [UNESP]
Rocha, TS [UNESP]
Menconi, A [UNESP]
Marietto-Gonçalves, GA [UNESP]
dc.subject.por.fl_str_mv Integron
gene transfer
resistance to antimicrobials
Salmonella Enteritidis
topic Integron
gene transfer
resistance to antimicrobials
Salmonella Enteritidis
description The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples were evaluated for their capacity to horizontally transfer this gene. There was no direct relationship between the presence of the class 1 integron gene and SE resistance to the 14 antimicrobials tested, as 80% of the studied samples were resistant to up to three antimicrobials, and did not present the aforementioned gene. However, horizontal transfer of this gene was accomplished in vitro (from Escherichia coli to Salmonella Enteritidis), demonstrating that capacity class 1 integron gene can be disseminated among enterobacteria.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-01
2014-05-20T13:39:29Z
2014-05-20T13:39:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-635X2009000300009
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 3, p. 195-201, 2009.
1516-635X
http://hdl.handle.net/11449/13692
10.1590/S1516-635X2009000300009
S1516-635X2009000300009
WOS:000272254000009
S1516-635X2009000300009.pdf
8502462873517464
url http://dx.doi.org/10.1590/S1516-635X2009000300009
http://hdl.handle.net/11449/13692
identifier_str_mv Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 3, p. 195-201, 2009.
1516-635X
10.1590/S1516-635X2009000300009
S1516-635X2009000300009
WOS:000272254000009
S1516-635X2009000300009.pdf
8502462873517464
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Ciência Avícola
0.463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 195-201
application/pdf
dc.publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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