The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1371/journal.pone.0247392 |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0247392 http://hdl.handle.net/11449/209224 |
Resumo: | Purpose The ocular surface microbiome has been described as paucibacterial. Until now, studies investigating the bacterial community associated with the ocular surface through high-throughput sequencing have focused on the conjunctiva. Conjunctival samples are thought to reflect and be representative of the microbiome residing on the ocular surface, including the cornea. Here, we hypothesized that the bacterial community associated with the corneal surface was different from those of the inferonasal and superotemporal conjunctival fornices, and from the tear film. Methods Both eyes from 15 healthy piglets were sampled using swabs (inferonasal fornix, superotemporal fornix, and corneal surface, n = 30 each) and Schirmer tear test strips (STT, n = 30). Negative sampling controls (swabs and STT, n = 2 each) and extraction controls (n = 4) were included. Total DNA was extracted and high-throughput sequencing targeting the 16S rRNA gene was performed. Bioinformatic analyses included multiple contamination-controlling steps. Results Corneal surface samples had a significantly lower number of taxa detected (P<0.01) and were compositionally different from all other sample types (Bray-Curtis dissimilarity, P<0.04). It also harbored higher levels of Proteobacteria (P<0.05), specifically Brevundimonas spp. (4.1-fold) and Paracoccus spp. (3.4-fold) than other sample types. Negative control STT strip samples yielded the highest amount of 16S rRNA gene copies across all sample types (P<0.05). Conclusions Our data suggests that the corneal surface provides a distinct environmental niche within the ocular surface, leading to a bacterial community compositionally different from all other sample types. |
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The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surfacePurpose The ocular surface microbiome has been described as paucibacterial. Until now, studies investigating the bacterial community associated with the ocular surface through high-throughput sequencing have focused on the conjunctiva. Conjunctival samples are thought to reflect and be representative of the microbiome residing on the ocular surface, including the cornea. Here, we hypothesized that the bacterial community associated with the corneal surface was different from those of the inferonasal and superotemporal conjunctival fornices, and from the tear film. Methods Both eyes from 15 healthy piglets were sampled using swabs (inferonasal fornix, superotemporal fornix, and corneal surface, n = 30 each) and Schirmer tear test strips (STT, n = 30). Negative sampling controls (swabs and STT, n = 2 each) and extraction controls (n = 4) were included. Total DNA was extracted and high-throughput sequencing targeting the 16S rRNA gene was performed. Bioinformatic analyses included multiple contamination-controlling steps. Results Corneal surface samples had a significantly lower number of taxa detected (P<0.01) and were compositionally different from all other sample types (Bray-Curtis dissimilarity, P<0.04). It also harbored higher levels of Proteobacteria (P<0.05), specifically Brevundimonas spp. (4.1-fold) and Paracoccus spp. (3.4-fold) than other sample types. Negative control STT strip samples yielded the highest amount of 16S rRNA gene copies across all sample types (P<0.05). Conclusions Our data suggests that the corneal surface provides a distinct environmental niche within the ocular surface, leading to a bacterial community compositionally different from all other sample types.department of Veterinary Clinical Sciences (VCS) at the University of Minnesota (UMN)Univ Minnesota, Coll Vet Med, Vet Clin Sci, Minneapolis, MN 55455 USAUniv Saskatchewan, Western Coll Vet Med, Small Anim Clin Sci, Saskatoon, SK, CanadaState Univ Sao Paulo, Sao Paulo, BrazilUniv Saskatchewan, Western Coll Vet Med, Large Anim Clin Sci, Saskatoon, SK, CanadaUniv Utrecht, Fac Vet Med, Dept Populat Hlth, Utrecht, NetherlandsState Univ Sao Paulo, Sao Paulo, BrazilPublic Library ScienceUniv MinnesotaUniv SaskatchewanUniversidade Estadual Paulista (Unesp)Univ UtrechtLeis, Marina L.Madruga, Gabriela M. [UNESP]Costa, Matheus O.2021-06-25T11:52:02Z2021-06-25T11:52:02Z2021-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16http://dx.doi.org/10.1371/journal.pone.0247392Plos One. San Francisco: Public Library Science, v. 16, n. 2, 16 p., 2021.1932-6203http://hdl.handle.net/11449/20922410.1371/journal.pone.0247392WOS:000620629200128Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2021-10-23T19:23:40Zoai:repositorio.unesp.br:11449/209224Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:49:26.838556Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
title |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
spellingShingle |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface Leis, Marina L. Leis, Marina L. |
title_short |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
title_full |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
title_fullStr |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
title_full_unstemmed |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
title_sort |
The porcine corneal surface bacterial microbiome: A distinctive niche within the ocular surface |
author |
Leis, Marina L. |
author_facet |
Leis, Marina L. Leis, Marina L. Madruga, Gabriela M. [UNESP] Costa, Matheus O. Madruga, Gabriela M. [UNESP] Costa, Matheus O. |
author_role |
author |
author2 |
Madruga, Gabriela M. [UNESP] Costa, Matheus O. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Univ Minnesota Univ Saskatchewan Universidade Estadual Paulista (Unesp) Univ Utrecht |
dc.contributor.author.fl_str_mv |
Leis, Marina L. Madruga, Gabriela M. [UNESP] Costa, Matheus O. |
description |
Purpose The ocular surface microbiome has been described as paucibacterial. Until now, studies investigating the bacterial community associated with the ocular surface through high-throughput sequencing have focused on the conjunctiva. Conjunctival samples are thought to reflect and be representative of the microbiome residing on the ocular surface, including the cornea. Here, we hypothesized that the bacterial community associated with the corneal surface was different from those of the inferonasal and superotemporal conjunctival fornices, and from the tear film. Methods Both eyes from 15 healthy piglets were sampled using swabs (inferonasal fornix, superotemporal fornix, and corneal surface, n = 30 each) and Schirmer tear test strips (STT, n = 30). Negative sampling controls (swabs and STT, n = 2 each) and extraction controls (n = 4) were included. Total DNA was extracted and high-throughput sequencing targeting the 16S rRNA gene was performed. Bioinformatic analyses included multiple contamination-controlling steps. Results Corneal surface samples had a significantly lower number of taxa detected (P<0.01) and were compositionally different from all other sample types (Bray-Curtis dissimilarity, P<0.04). It also harbored higher levels of Proteobacteria (P<0.05), specifically Brevundimonas spp. (4.1-fold) and Paracoccus spp. (3.4-fold) than other sample types. Negative control STT strip samples yielded the highest amount of 16S rRNA gene copies across all sample types (P<0.05). Conclusions Our data suggests that the corneal surface provides a distinct environmental niche within the ocular surface, leading to a bacterial community compositionally different from all other sample types. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:52:02Z 2021-06-25T11:52:02Z 2021-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0247392 Plos One. San Francisco: Public Library Science, v. 16, n. 2, 16 p., 2021. 1932-6203 http://hdl.handle.net/11449/209224 10.1371/journal.pone.0247392 WOS:000620629200128 |
url |
http://dx.doi.org/10.1371/journal.pone.0247392 http://hdl.handle.net/11449/209224 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 16, n. 2, 16 p., 2021. 1932-6203 10.1371/journal.pone.0247392 WOS:000620629200128 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos One |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1822182297020923904 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0247392 |