Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes

• Autor(a) principal: Mathias, Lucas Solla [UNESP]
• Data de Publicação: 2020
• Outros Autores: Rodrigues, Bruna Moretto [UNESP], Gonçalves, Bianca Mariani [UNESP], Moretto, Fernanda Cristina Fontes [UNESP], Olimpio, Regiane Marques Castro [UNESP], Deprá, Igor [UNESP], De Sibio, Maria Teresa [UNESP], Tilli, Helena Paim [UNESP], Nogueira, Célia Regina [UNESP], de Oliveira, Miriane [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.mce.2019.110690; http://hdl.handle.net/11449/201427
• topic: Adiponectin; Integrin αVβ3; Leptin; Lipid; MAPK/ERK; Triiodothyronine

Adiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation [glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPARγ protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin αvβ3 to alter the leptin gene expression.