Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00424-019-02303-1 http://hdl.handle.net/11449/190597 |
Resumo: | Recently, we have described, in non-genetically modified rats, that peripheral transient receptor potential vanilloid-4 (TRPV4) channels are activated and trigger warmth-defence responses at ambient temperatures of 26–30 °C. Evidence points to the presence of TRPV4 in the medial preoptic area, a region described to be involved in the activation of thermoeffector pathways, including those involved in heat loss. Thus, we tested the hypothesis that TRPV4 in the medial preoptic area modulates thermoregulation under warm conditions. To this end, under two ambient temperatures (21 and 28 °C), body temperature was measured in rats following blockade of preoptic TRPV4 with two antagonists, HC-067047 and GSK 2193874. Oxygen consumption, heat loss index and preferred ambient temperature were also determined in order to assess thermoeffector activity. Antagonism of central TRPV4 caused an increase in body temperature in rats exposed to 28 °C, but not in those exposed to 21 °C. The body temperature increase at 28 °C was accompanied by an increase in oxygen consumption and an earlier reduction of the heat loss index. In behavioural experiments, control animals previously exposed to warm ambient temperatures (28–30 °C) for 2 h selected colder temperatures in a thermogradient compared to those injected with HC-067047. Our results support the idea that preoptic TRPV4 modulates thermoregulation in a warm environment by activating both autonomic and behavioural heat loss responses. Thus, according to the present study and to that published recently by our group, the activation of warmth-defence responses by TRPV4 seems to be dependent on the activity of both peripheral and central channels. |
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Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male ratsBehavioural thermoregulationBody temperatureGSK 2193874HC-067047Heat loss indexOxygen consumptionRecently, we have described, in non-genetically modified rats, that peripheral transient receptor potential vanilloid-4 (TRPV4) channels are activated and trigger warmth-defence responses at ambient temperatures of 26–30 °C. Evidence points to the presence of TRPV4 in the medial preoptic area, a region described to be involved in the activation of thermoeffector pathways, including those involved in heat loss. Thus, we tested the hypothesis that TRPV4 in the medial preoptic area modulates thermoregulation under warm conditions. To this end, under two ambient temperatures (21 and 28 °C), body temperature was measured in rats following blockade of preoptic TRPV4 with two antagonists, HC-067047 and GSK 2193874. Oxygen consumption, heat loss index and preferred ambient temperature were also determined in order to assess thermoeffector activity. Antagonism of central TRPV4 caused an increase in body temperature in rats exposed to 28 °C, but not in those exposed to 21 °C. The body temperature increase at 28 °C was accompanied by an increase in oxygen consumption and an earlier reduction of the heat loss index. In behavioural experiments, control animals previously exposed to warm ambient temperatures (28–30 °C) for 2 h selected colder temperatures in a thermogradient compared to those injected with HC-067047. Our results support the idea that preoptic TRPV4 modulates thermoregulation in a warm environment by activating both autonomic and behavioural heat loss responses. Thus, according to the present study and to that published recently by our group, the activation of warmth-defence responses by TRPV4 seems to be dependent on the activity of both peripheral and central channels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Animal Morphology and Physiology College of Agricultural and Veterinary Sciences Sao Paulo State University, Via de Acesso Prof. Paulo D. Castellane, s/n; Bairro RuralJoint UFSCar-UNESP Graduate Program of Physiological SciencesKeenan Research Centre for Biomedical Science St. Michael’s HospitalDepartment of Physiology Faculty of Medicine and Dentistry University of AlbertaCenter for Natural and Human Sciences Universidade Federal do ABC (UFABC)Department of Animal Morphology and Physiology College of Agricultural and Veterinary Sciences Sao Paulo State University, Via de Acesso Prof. Paulo D. Castellane, s/n; Bairro RuralJoint UFSCar-UNESP Graduate Program of Physiological SciencesFAPESP: 15/02991-0FAPESP: 15/04849-6FAPESP: 2011/19131-2FAPESP: 2012/15298-2FAPESP: 2017/17278-2CNPq: 442560/2014-1Universidade Estadual Paulista (Unesp)St. Michael’s HospitalUniversity of AlbertaUniversidade Federal do ABC (UFABC)Scarpellini, Carolina da Silveira [UNESP]Cristina-Silva, Caroline [UNESP]Biancardi, Vivian [UNESP]Gargaglioni, Luciane H. [UNESP]Almeida, Maria CamilaBícego, Kênia Cardoso [UNESP]2019-10-06T17:18:32Z2019-10-06T17:18:32Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1191-1203http://dx.doi.org/10.1007/s00424-019-02303-1Pflugers Archiv European Journal of Physiology, v. 471, n. 9, p. 1191-1203, 2019.1432-20130031-6768http://hdl.handle.net/11449/19059710.1007/s00424-019-02303-12-s2.0-85071091532Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPflugers Archiv European Journal of Physiologyinfo:eu-repo/semantics/openAccess2024-06-06T18:41:13Zoai:repositorio.unesp.br:11449/190597Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:24:44.773618Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
title |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
spellingShingle |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats Scarpellini, Carolina da Silveira [UNESP] Behavioural thermoregulation Body temperature GSK 2193874 HC-067047 Heat loss index Oxygen consumption |
title_short |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
title_full |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
title_fullStr |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
title_full_unstemmed |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
title_sort |
Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats |
author |
Scarpellini, Carolina da Silveira [UNESP] |
author_facet |
Scarpellini, Carolina da Silveira [UNESP] Cristina-Silva, Caroline [UNESP] Biancardi, Vivian [UNESP] Gargaglioni, Luciane H. [UNESP] Almeida, Maria Camila Bícego, Kênia Cardoso [UNESP] |
author_role |
author |
author2 |
Cristina-Silva, Caroline [UNESP] Biancardi, Vivian [UNESP] Gargaglioni, Luciane H. [UNESP] Almeida, Maria Camila Bícego, Kênia Cardoso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) St. Michael’s Hospital University of Alberta Universidade Federal do ABC (UFABC) |
dc.contributor.author.fl_str_mv |
Scarpellini, Carolina da Silveira [UNESP] Cristina-Silva, Caroline [UNESP] Biancardi, Vivian [UNESP] Gargaglioni, Luciane H. [UNESP] Almeida, Maria Camila Bícego, Kênia Cardoso [UNESP] |
dc.subject.por.fl_str_mv |
Behavioural thermoregulation Body temperature GSK 2193874 HC-067047 Heat loss index Oxygen consumption |
topic |
Behavioural thermoregulation Body temperature GSK 2193874 HC-067047 Heat loss index Oxygen consumption |
description |
Recently, we have described, in non-genetically modified rats, that peripheral transient receptor potential vanilloid-4 (TRPV4) channels are activated and trigger warmth-defence responses at ambient temperatures of 26–30 °C. Evidence points to the presence of TRPV4 in the medial preoptic area, a region described to be involved in the activation of thermoeffector pathways, including those involved in heat loss. Thus, we tested the hypothesis that TRPV4 in the medial preoptic area modulates thermoregulation under warm conditions. To this end, under two ambient temperatures (21 and 28 °C), body temperature was measured in rats following blockade of preoptic TRPV4 with two antagonists, HC-067047 and GSK 2193874. Oxygen consumption, heat loss index and preferred ambient temperature were also determined in order to assess thermoeffector activity. Antagonism of central TRPV4 caused an increase in body temperature in rats exposed to 28 °C, but not in those exposed to 21 °C. The body temperature increase at 28 °C was accompanied by an increase in oxygen consumption and an earlier reduction of the heat loss index. In behavioural experiments, control animals previously exposed to warm ambient temperatures (28–30 °C) for 2 h selected colder temperatures in a thermogradient compared to those injected with HC-067047. Our results support the idea that preoptic TRPV4 modulates thermoregulation in a warm environment by activating both autonomic and behavioural heat loss responses. Thus, according to the present study and to that published recently by our group, the activation of warmth-defence responses by TRPV4 seems to be dependent on the activity of both peripheral and central channels. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:18:32Z 2019-10-06T17:18:32Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00424-019-02303-1 Pflugers Archiv European Journal of Physiology, v. 471, n. 9, p. 1191-1203, 2019. 1432-2013 0031-6768 http://hdl.handle.net/11449/190597 10.1007/s00424-019-02303-1 2-s2.0-85071091532 |
url |
http://dx.doi.org/10.1007/s00424-019-02303-1 http://hdl.handle.net/11449/190597 |
identifier_str_mv |
Pflugers Archiv European Journal of Physiology, v. 471, n. 9, p. 1191-1203, 2019. 1432-2013 0031-6768 10.1007/s00424-019-02303-1 2-s2.0-85071091532 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pflugers Archiv European Journal of Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1191-1203 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128356402593792 |