Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pharmaceutics13070998 http://hdl.handle.net/11449/233261 |
Resumo: | Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation. |
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Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitroAnti-Candida activityBiofilmCandida albicansEnterococ-cus faeciumOrodispersible filmsProbioticsBackground: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Pharmaceutical Sciences Sao Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 01-s/n, Campus VilleReference Center for Lactobacilli (CERELA-CONICET) San Miguel de Tucumán ILC, Chacabuco 145School of Pharmaceutical Sciences Sao Paulo State University (UNESP), Rodovia Araraquara-Jaú, Km 01-s/n, Campus VilleFAPESP: 2018/09088-1FAPESP: 2018/23015-7Universidade Estadual Paulista (UNESP)ILCLordello, Virgínia Barreto [UNESP]Meneguin, Andréia Bagliotti [UNESP]de Annunzio, Sarah Raquel [UNESP]Taranto, Maria PíaChorilli, Marlus [UNESP]Fontana, Carla Raquel [UNESP]Cavallini, Daniela Cardoso Umbelino [UNESP]2022-05-01T06:02:18Z2022-05-01T06:02:18Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pharmaceutics13070998Pharmaceutics, v. 13, n. 7, 2021.1999-4923http://hdl.handle.net/11449/23326110.3390/pharmaceutics130709982-s2.0-85110030514Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceuticsinfo:eu-repo/semantics/openAccess2024-06-24T13:45:18Zoai:repositorio.unesp.br:11449/233261Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T13:45:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
title |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
spellingShingle |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro Lordello, Virgínia Barreto [UNESP] Anti-Candida activity Biofilm Candida albicans Enterococ-cus faecium Orodispersible films Probiotics |
title_short |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
title_full |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
title_fullStr |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
title_full_unstemmed |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
title_sort |
Orodispersible film loaded with enterococcus faecium crl183 presents anti-candida albicans biofilm activity in vitro |
author |
Lordello, Virgínia Barreto [UNESP] |
author_facet |
Lordello, Virgínia Barreto [UNESP] Meneguin, Andréia Bagliotti [UNESP] de Annunzio, Sarah Raquel [UNESP] Taranto, Maria Pía Chorilli, Marlus [UNESP] Fontana, Carla Raquel [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
author_role |
author |
author2 |
Meneguin, Andréia Bagliotti [UNESP] de Annunzio, Sarah Raquel [UNESP] Taranto, Maria Pía Chorilli, Marlus [UNESP] Fontana, Carla Raquel [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) ILC |
dc.contributor.author.fl_str_mv |
Lordello, Virgínia Barreto [UNESP] Meneguin, Andréia Bagliotti [UNESP] de Annunzio, Sarah Raquel [UNESP] Taranto, Maria Pía Chorilli, Marlus [UNESP] Fontana, Carla Raquel [UNESP] Cavallini, Daniela Cardoso Umbelino [UNESP] |
dc.subject.por.fl_str_mv |
Anti-Candida activity Biofilm Candida albicans Enterococ-cus faecium Orodispersible films Probiotics |
topic |
Anti-Candida activity Biofilm Candida albicans Enterococ-cus faecium Orodispersible films Probiotics |
description |
Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1 ), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-05-01T06:02:18Z 2022-05-01T06:02:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pharmaceutics13070998 Pharmaceutics, v. 13, n. 7, 2021. 1999-4923 http://hdl.handle.net/11449/233261 10.3390/pharmaceutics13070998 2-s2.0-85110030514 |
url |
http://dx.doi.org/10.3390/pharmaceutics13070998 http://hdl.handle.net/11449/233261 |
identifier_str_mv |
Pharmaceutics, v. 13, n. 7, 2021. 1999-4923 10.3390/pharmaceutics13070998 2-s2.0-85110030514 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045256544911360 |