Efeito da co-cultura de fibroblastos NIH3T3 e mioblastos C2C12 na expressão da osteoglicina e na migração celular

• Autor(a) principal: Cury, Sarah Santiloni [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/203860; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-01-28/000898066.pdf
• topic: Distrofia; Sistema musculoesquelético; Fibroblastos; Dystrophy

In mammals, the skeletal muscle growth and regeneration are assigned to process called myogenesis that is regulated, at molecular level, by the interaction of intra and extra-cellular signal transducers. Skeletal muscle differentiation, or myogenesis, is a highly-orchestrated process in which mononucleated muscle precursor cells, the myoblasts, undergo proliferation. Upon differentiation, they withdraw from the cell cycle, migrate, align with each other, and subsequently fuse to form terminally differentiated multinucleated myotubes. Intracellular factors that control this process are already well known, however, recent studies have shown that the myogenic process is highly sensitive and regulated by molecules present in the extracellular medium, even for components secreted by muscle cells (secretome). Recent data from our laboratory demonstrate that among the main components of secretome, the osteoglicina (Ogn) has an important role in myogenesis because the post transcriptional silencing of Ogn by small interfering RNAs (siRNAs) alters the proliferation and differentiation of myoblasts C2C12. Furthermore, Ogn has been described as a major protein secreted by fibroblasts to the extracellular matrix and for presenting function in the aging of heart fibroblasts. Although it is already described certain features of Ogn in fibroblasts or muscle cells, it is still unknown whether the Ogn can participate in one way of communication between these two cell types. Therefore, in this study, we tested the hypothesis that co-culture of fibroblasts NIH3T3 (FB) and myoblasts C2C12 (MB) changes the protein levels of Ogn and consequently myoblast migration. For this, we used the co-cultures FB/MB and MB/MB for 0h, 12h and 24 h to evaluate, in myoblasts, cell migration and protein levels of Ogn. The co-culture FB/MB does not alter the myoblast migration rate; however, it decreased the Ogn protein levels after 12h and...