Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads

Detalhes bibliográficos
Autor(a) principal: Paz-Cedeno, Fernando Roberto [UNESP]
Data de Publicação: 2021
Outros Autores: Henares, Lucas Ragnini [UNESP], Solorzano-Chavez, Eddyn Gabriel [UNESP], Scontri, Mateus [UNESP], Picheli, Flávio Pereira [UNESP], Miranda Roldán, Ismael Ulises [UNESP], Monti, Rubens [UNESP], Conceição de Oliveira, Samuel [UNESP], Masarin, Fernando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.renene.2020.10.092
http://hdl.handle.net/11449/208151
Resumo: In the present study, sugarcane bagasse (SB) was subjected to different pretreatments. The pretreated SB was characterized chemically and structurally and was enzymatically hydrolyzed using a commercial enzyme preparation. Pretreatment with sulfite-NaOH was the most efficient for removing lignin while keeping cellulose intact. In addition, sulfite-NaOH pretreatment presented the best response to the enzymatic hydrolysis of cellulose and xylan, reaching conversions of 90%. The increase in consistency (≥10%) in the enzymatic hydrolysis of SB pretreated with sulfite-NaOH showed a loss of cellulose and xylan conversions efficiencies of 28 and 37%, respectively. However, enzymatic hydrolysis with a consistency of 20% resulted in a maximum rate of glucose and xylose formation of 8.5 and 3.0 g L−1 h−1, respectively, and an enzymatic hydrolysate containing 80 and 33 g L−1 of glucose and xylose, respectively. The enzymatic hydrolysis assay in a bioreactor with 20% consistency promoted faster liquefaction of SB, resulting in a higher maximum rate of glucose production (10.6 g L−1 h−1). The increase in the concentration and rate of formation of fermentable sugars in the enzymatic hydrolysate can partially avoid steps of concentration of the hydrolysate, resulting in less energy consumption and greater productivity of the bioproducts obtained from the hydrolysate, such as cellulosic ethanol (2G ethanol).
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spelling Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loadsAlkaline pretreatmentBiomass hydrolysisCellulaseHigh consistencyLignocellulosic biomassXylanaseIn the present study, sugarcane bagasse (SB) was subjected to different pretreatments. The pretreated SB was characterized chemically and structurally and was enzymatically hydrolyzed using a commercial enzyme preparation. Pretreatment with sulfite-NaOH was the most efficient for removing lignin while keeping cellulose intact. In addition, sulfite-NaOH pretreatment presented the best response to the enzymatic hydrolysis of cellulose and xylan, reaching conversions of 90%. The increase in consistency (≥10%) in the enzymatic hydrolysis of SB pretreated with sulfite-NaOH showed a loss of cellulose and xylan conversions efficiencies of 28 and 37%, respectively. However, enzymatic hydrolysis with a consistency of 20% resulted in a maximum rate of glucose and xylose formation of 8.5 and 3.0 g L−1 h−1, respectively, and an enzymatic hydrolysate containing 80 and 33 g L−1 of glucose and xylose, respectively. The enzymatic hydrolysis assay in a bioreactor with 20% consistency promoted faster liquefaction of SB, resulting in a higher maximum rate of glucose production (10.6 g L−1 h−1). The increase in the concentration and rate of formation of fermentable sugars in the enzymatic hydrolysate can partially avoid steps of concentration of the hydrolysate, resulting in less energy consumption and greater productivity of the bioproducts obtained from the hydrolysate, such as cellulosic ethanol (2G ethanol).Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Pharmaceutical Sciences (FCF) Department of Bioprocess Engineering and BiotechnologySão Paulo State University (UNESP) School of Pharmaceutical Sciences (FCF) Department of Bioprocess Engineering and BiotechnologyUniversidade Estadual Paulista (Unesp)Paz-Cedeno, Fernando Roberto [UNESP]Henares, Lucas Ragnini [UNESP]Solorzano-Chavez, Eddyn Gabriel [UNESP]Scontri, Mateus [UNESP]Picheli, Flávio Pereira [UNESP]Miranda Roldán, Ismael Ulises [UNESP]Monti, Rubens [UNESP]Conceição de Oliveira, Samuel [UNESP]Masarin, Fernando [UNESP]2021-06-25T11:07:15Z2021-06-25T11:07:15Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13http://dx.doi.org/10.1016/j.renene.2020.10.092Renewable Energy, v. 165, p. 1-13.1879-06820960-1481http://hdl.handle.net/11449/20815110.1016/j.renene.2020.10.0922-s2.0-85096213992Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRenewable Energyinfo:eu-repo/semantics/openAccess2024-06-21T12:46:36Zoai:repositorio.unesp.br:11449/208151Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:18:16.599870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
title Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
spellingShingle Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
Paz-Cedeno, Fernando Roberto [UNESP]
Alkaline pretreatment
Biomass hydrolysis
Cellulase
High consistency
Lignocellulosic biomass
Xylanase
title_short Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
title_full Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
title_fullStr Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
title_full_unstemmed Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
title_sort Evaluation of the effects of different chemical pretreatments in sugarcane bagasse on the response of enzymatic hydrolysis in batch systems subject to high mass loads
author Paz-Cedeno, Fernando Roberto [UNESP]
author_facet Paz-Cedeno, Fernando Roberto [UNESP]
Henares, Lucas Ragnini [UNESP]
Solorzano-Chavez, Eddyn Gabriel [UNESP]
Scontri, Mateus [UNESP]
Picheli, Flávio Pereira [UNESP]
Miranda Roldán, Ismael Ulises [UNESP]
Monti, Rubens [UNESP]
Conceição de Oliveira, Samuel [UNESP]
Masarin, Fernando [UNESP]
author_role author
author2 Henares, Lucas Ragnini [UNESP]
Solorzano-Chavez, Eddyn Gabriel [UNESP]
Scontri, Mateus [UNESP]
Picheli, Flávio Pereira [UNESP]
Miranda Roldán, Ismael Ulises [UNESP]
Monti, Rubens [UNESP]
Conceição de Oliveira, Samuel [UNESP]
Masarin, Fernando [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Paz-Cedeno, Fernando Roberto [UNESP]
Henares, Lucas Ragnini [UNESP]
Solorzano-Chavez, Eddyn Gabriel [UNESP]
Scontri, Mateus [UNESP]
Picheli, Flávio Pereira [UNESP]
Miranda Roldán, Ismael Ulises [UNESP]
Monti, Rubens [UNESP]
Conceição de Oliveira, Samuel [UNESP]
Masarin, Fernando [UNESP]
dc.subject.por.fl_str_mv Alkaline pretreatment
Biomass hydrolysis
Cellulase
High consistency
Lignocellulosic biomass
Xylanase
topic Alkaline pretreatment
Biomass hydrolysis
Cellulase
High consistency
Lignocellulosic biomass
Xylanase
description In the present study, sugarcane bagasse (SB) was subjected to different pretreatments. The pretreated SB was characterized chemically and structurally and was enzymatically hydrolyzed using a commercial enzyme preparation. Pretreatment with sulfite-NaOH was the most efficient for removing lignin while keeping cellulose intact. In addition, sulfite-NaOH pretreatment presented the best response to the enzymatic hydrolysis of cellulose and xylan, reaching conversions of 90%. The increase in consistency (≥10%) in the enzymatic hydrolysis of SB pretreated with sulfite-NaOH showed a loss of cellulose and xylan conversions efficiencies of 28 and 37%, respectively. However, enzymatic hydrolysis with a consistency of 20% resulted in a maximum rate of glucose and xylose formation of 8.5 and 3.0 g L−1 h−1, respectively, and an enzymatic hydrolysate containing 80 and 33 g L−1 of glucose and xylose, respectively. The enzymatic hydrolysis assay in a bioreactor with 20% consistency promoted faster liquefaction of SB, resulting in a higher maximum rate of glucose production (10.6 g L−1 h−1). The increase in the concentration and rate of formation of fermentable sugars in the enzymatic hydrolysate can partially avoid steps of concentration of the hydrolysate, resulting in less energy consumption and greater productivity of the bioproducts obtained from the hydrolysate, such as cellulosic ethanol (2G ethanol).
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:07:15Z
2021-06-25T11:07:15Z
2021-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.renene.2020.10.092
Renewable Energy, v. 165, p. 1-13.
1879-0682
0960-1481
http://hdl.handle.net/11449/208151
10.1016/j.renene.2020.10.092
2-s2.0-85096213992
url http://dx.doi.org/10.1016/j.renene.2020.10.092
http://hdl.handle.net/11449/208151
identifier_str_mv Renewable Energy, v. 165, p. 1-13.
1879-0682
0960-1481
10.1016/j.renene.2020.10.092
2-s2.0-85096213992
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Renewable Energy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-13
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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