Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10815-018-1365-4 http://hdl.handle.net/11449/188371 |
Resumo: | Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence. |
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Simulated physiological oocyte maturation has side effects on bovine oocytes and embryosBovine blastocystGene expressionIn vitro maturationSPOMUltrastructurePurpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)InnovationsfondenDepartment of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/nDepartment of Animal Science Aarhus UniversityEmbryoTrans Biotech, Frederiksberg CDepartment of Bio and Health Informatics Technical University of Denmark, KemitorvetDepartment of Veterinary and Animal Sciences University of CopenhagenDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100Department of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/nDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100FAPESP: #12234099FAPESP: #12505332FAPESP: #13050831Universidade Estadual Paulista (Unesp)Aarhus UniversityEmbryoTrans BiotechTechnical University of DenmarkUniversity of CopenhagenRazza, Eduardo M. [UNESP]Pedersen, Hanne S.Stroebech, LotteFontes, Patricia K. [UNESP]Kadarmideen, Haja N.Callesen, HenrikPihl, MariaNogueira, Marcelo F. G. [UNESP]Hyttel, Poul2019-10-06T16:05:55Z2019-10-06T16:05:55Z2019-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article413-424http://dx.doi.org/10.1007/s10815-018-1365-4Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019.1573-73301058-0468http://hdl.handle.net/11449/18837110.1007/s10815-018-1365-42-s2.0-85056720403Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Assisted Reproduction and Geneticsinfo:eu-repo/semantics/openAccess2021-10-22T21:16:10Zoai:repositorio.unesp.br:11449/188371Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:18.372571Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
title |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
spellingShingle |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos Razza, Eduardo M. [UNESP] Bovine blastocyst Gene expression In vitro maturation SPOM Ultrastructure |
title_short |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
title_full |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
title_fullStr |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
title_full_unstemmed |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
title_sort |
Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos |
author |
Razza, Eduardo M. [UNESP] |
author_facet |
Razza, Eduardo M. [UNESP] Pedersen, Hanne S. Stroebech, Lotte Fontes, Patricia K. [UNESP] Kadarmideen, Haja N. Callesen, Henrik Pihl, Maria Nogueira, Marcelo F. G. [UNESP] Hyttel, Poul |
author_role |
author |
author2 |
Pedersen, Hanne S. Stroebech, Lotte Fontes, Patricia K. [UNESP] Kadarmideen, Haja N. Callesen, Henrik Pihl, Maria Nogueira, Marcelo F. G. [UNESP] Hyttel, Poul |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Aarhus University EmbryoTrans Biotech Technical University of Denmark University of Copenhagen |
dc.contributor.author.fl_str_mv |
Razza, Eduardo M. [UNESP] Pedersen, Hanne S. Stroebech, Lotte Fontes, Patricia K. [UNESP] Kadarmideen, Haja N. Callesen, Henrik Pihl, Maria Nogueira, Marcelo F. G. [UNESP] Hyttel, Poul |
dc.subject.por.fl_str_mv |
Bovine blastocyst Gene expression In vitro maturation SPOM Ultrastructure |
topic |
Bovine blastocyst Gene expression In vitro maturation SPOM Ultrastructure |
description |
Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:05:55Z 2019-10-06T16:05:55Z 2019-03-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10815-018-1365-4 Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019. 1573-7330 1058-0468 http://hdl.handle.net/11449/188371 10.1007/s10815-018-1365-4 2-s2.0-85056720403 |
url |
http://dx.doi.org/10.1007/s10815-018-1365-4 http://hdl.handle.net/11449/188371 |
identifier_str_mv |
Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019. 1573-7330 1058-0468 10.1007/s10815-018-1365-4 2-s2.0-85056720403 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Assisted Reproduction and Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
413-424 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128961079672832 |