Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos

Detalhes bibliográficos
Autor(a) principal: Razza, Eduardo M. [UNESP]
Data de Publicação: 2019
Outros Autores: Pedersen, Hanne S., Stroebech, Lotte, Fontes, Patricia K. [UNESP], Kadarmideen, Haja N., Callesen, Henrik, Pihl, Maria, Nogueira, Marcelo F. G. [UNESP], Hyttel, Poul
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10815-018-1365-4
http://hdl.handle.net/11449/188371
Resumo: Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence.
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spelling Simulated physiological oocyte maturation has side effects on bovine oocytes and embryosBovine blastocystGene expressionIn vitro maturationSPOMUltrastructurePurpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)InnovationsfondenDepartment of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/nDepartment of Animal Science Aarhus UniversityEmbryoTrans Biotech, Frederiksberg CDepartment of Bio and Health Informatics Technical University of Denmark, KemitorvetDepartment of Veterinary and Animal Sciences University of CopenhagenDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100Department of Pharmacology Institute of Bioscience São Paulo State University (UNESP), Distrito de Rubião Junior s/nDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), Avenida Dom Antonio, 2100FAPESP: #12234099FAPESP: #12505332FAPESP: #13050831Universidade Estadual Paulista (Unesp)Aarhus UniversityEmbryoTrans BiotechTechnical University of DenmarkUniversity of CopenhagenRazza, Eduardo M. [UNESP]Pedersen, Hanne S.Stroebech, LotteFontes, Patricia K. [UNESP]Kadarmideen, Haja N.Callesen, HenrikPihl, MariaNogueira, Marcelo F. G. [UNESP]Hyttel, Poul2019-10-06T16:05:55Z2019-10-06T16:05:55Z2019-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article413-424http://dx.doi.org/10.1007/s10815-018-1365-4Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019.1573-73301058-0468http://hdl.handle.net/11449/18837110.1007/s10815-018-1365-42-s2.0-85056720403Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Assisted Reproduction and Geneticsinfo:eu-repo/semantics/openAccess2021-10-22T21:16:10Zoai:repositorio.unesp.br:11449/188371Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:18.372571Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
title Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
spellingShingle Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
Razza, Eduardo M. [UNESP]
Bovine blastocyst
Gene expression
In vitro maturation
SPOM
Ultrastructure
title_short Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
title_full Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
title_fullStr Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
title_full_unstemmed Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
title_sort Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos
author Razza, Eduardo M. [UNESP]
author_facet Razza, Eduardo M. [UNESP]
Pedersen, Hanne S.
Stroebech, Lotte
Fontes, Patricia K. [UNESP]
Kadarmideen, Haja N.
Callesen, Henrik
Pihl, Maria
Nogueira, Marcelo F. G. [UNESP]
Hyttel, Poul
author_role author
author2 Pedersen, Hanne S.
Stroebech, Lotte
Fontes, Patricia K. [UNESP]
Kadarmideen, Haja N.
Callesen, Henrik
Pihl, Maria
Nogueira, Marcelo F. G. [UNESP]
Hyttel, Poul
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Aarhus University
EmbryoTrans Biotech
Technical University of Denmark
University of Copenhagen
dc.contributor.author.fl_str_mv Razza, Eduardo M. [UNESP]
Pedersen, Hanne S.
Stroebech, Lotte
Fontes, Patricia K. [UNESP]
Kadarmideen, Haja N.
Callesen, Henrik
Pihl, Maria
Nogueira, Marcelo F. G. [UNESP]
Hyttel, Poul
dc.subject.por.fl_str_mv Bovine blastocyst
Gene expression
In vitro maturation
SPOM
Ultrastructure
topic Bovine blastocyst
Gene expression
In vitro maturation
SPOM
Ultrastructure
description Purpose: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently “primed” for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence. Methods: We tested the Simulated Physiological Oocyte Maturation (SPOM) system that uses forskolin and 3-isobutyl-1-methylxanthine for 2 h prior to IVM against two different systems of conventional IVM (Con-IVM). We evaluated the ultrastructure of matured oocytes and blastocysts and also assessed the expression of 96 genes related to embryo quality in the blastocysts. Results: In summary, the SPOM system resulted in lower blastocyst rates than both Con-IVM systems (30 ± 9.1 vs. 35 ± 8.7; 29 ± 2.6 vs. 38 ± 2.8). Mature SPOM oocytes had significantly increased volume and number of vesicles, reduced volume and surface density of large smooth endoplasmic reticulum clusters, and lower number of mitochondria than Con-IVM oocytes. SPOM blastocysts showed only subtle differences with parallel undulations of adjacent trophectoderm plasma membranes and peripherally localized ribosomes in cells of the inner cell mass compared with Con-IVM blastocysts. SPOM blastocysts, however, displayed significant downregulation of genes related to embryonic developmental potential when compared to Con-IVM blastocysts. Conclusions: Our results show that the use of the current version of the SPOM system may have adverse effects on oocytes and blastocysts calling for optimized protocols for improving oocyte competence.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:05:55Z
2019-10-06T16:05:55Z
2019-03-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10815-018-1365-4
Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019.
1573-7330
1058-0468
http://hdl.handle.net/11449/188371
10.1007/s10815-018-1365-4
2-s2.0-85056720403
url http://dx.doi.org/10.1007/s10815-018-1365-4
http://hdl.handle.net/11449/188371
identifier_str_mv Journal of Assisted Reproduction and Genetics, v. 36, n. 3, p. 413-424, 2019.
1573-7330
1058-0468
10.1007/s10815-018-1365-4
2-s2.0-85056720403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Assisted Reproduction and Genetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 413-424
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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