Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-022-04738-w http://hdl.handle.net/11449/247757 |
Resumo: | Objectives: To assess the influence of estrogen deficiency on tooth eruption rate (TER) and gene expression of estrogen receptor alpha and beta (ERα and ERβ) in the odontogenic region of teeth with continuous formation in a rat model. Materials and methods: Ovariectomies (OVX; n = 25) and sham surgeries (SHAM; n = 25) were performed in female Wistar rats when animals were 25 days old. The TER of the lower incisors, both in impeded (hyperfunction condition) and unimpeded (trimmed incisal edge—hypofunction condition) conditions, was evaluated using standardized digital photographs acquired every 48–72 h for 3 weeks (35th–53rd day of life), using a camera coupled to a stereomicroscope. Quantitative real-time PCR was performed to evaluate the relative gene expression of ERα and ERβ in the odontogenic region. Results: The OVX group showed a significant reduction in TER when compared to the SHAM group, only in the impeded condition (p = 0.03). There was no statistically significant difference between the groups in ERα gene expression (p = 0.33). ERβ showed a significantly higher gene expression in the OVX group (p ≤ 0.05). Conclusions: Estrogen deficiency decreases TER in teeth under impeded condition. Estrogen deficiency also increases ERβ gene expression in the odontogenic region of teeth with continuous formation. Clinical relevance: Hormonal disturbances affecting estrogen levels can cause alterations in dental formation and teeth eruption. |
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Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiencyEstrogen receptor alphaEstrogen receptor betaEstrogensOdontogenesisTooth eruptionObjectives: To assess the influence of estrogen deficiency on tooth eruption rate (TER) and gene expression of estrogen receptor alpha and beta (ERα and ERβ) in the odontogenic region of teeth with continuous formation in a rat model. Materials and methods: Ovariectomies (OVX; n = 25) and sham surgeries (SHAM; n = 25) were performed in female Wistar rats when animals were 25 days old. The TER of the lower incisors, both in impeded (hyperfunction condition) and unimpeded (trimmed incisal edge—hypofunction condition) conditions, was evaluated using standardized digital photographs acquired every 48–72 h for 3 weeks (35th–53rd day of life), using a camera coupled to a stereomicroscope. Quantitative real-time PCR was performed to evaluate the relative gene expression of ERα and ERβ in the odontogenic region. Results: The OVX group showed a significant reduction in TER when compared to the SHAM group, only in the impeded condition (p = 0.03). There was no statistically significant difference between the groups in ERα gene expression (p = 0.33). ERβ showed a significantly higher gene expression in the OVX group (p ≤ 0.05). Conclusions: Estrogen deficiency decreases TER in teeth under impeded condition. Estrogen deficiency also increases ERβ gene expression in the odontogenic region of teeth with continuous formation. Clinical relevance: Hormonal disturbances affecting estrogen levels can cause alterations in dental formation and teeth eruption.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Alexander von Humboldt-StiftungDepartment of Pediatric Dentistry School of Dentistry of Ribeirão Preto University of São Paulo Avenida Do Café S/N, Campus da USP, SPDepartment of Pediatric Dentistry and Orthodontics School of Dentistry Federal University of Rio de Janeiro Cidade Universitária, Rua Prof. Rodolpho Paulo Rocco 325, RJDepartment of Health Sciences and Pediatric Dentistry Piracicaba Dental School University of Campinas, Avenida Limeira 901, SPDepartment of Diagnosis and Surgery Araraquara Dental School São Paulo State University, Rua Humaitá, CentroSPDepartment of Stomatology Public Health and Forensic Dentistry School of Dentistry of Ribeirão Preto University of São Paulo Avenida Do Café S/N, Campus da USP, SPSchool of Dentistry University of the Joinville Region, Rua Paulo Malschitzki, s/n, SCDepartment of Biomolecular Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo Avenida Do Café S/N, Campus da USP, SPDepartment of Orthodontics University Medical Centre of Regensburg, Franz-Josef-Strauss-Allee 11Department of Diagnosis and Surgery Araraquara Dental School São Paulo State University, Rua Humaitá, CentroSPCAPES: 001FAPESP: 2015/06866-5FAPESP: 2016/08149-1Alexander von Humboldt-Stiftung: Küchler/Kirschneck 04th july 2019Universidade de São Paulo (USP)Cidade UniversitáriaUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)University of the Joinville RegionUniversity Medical Centre of RegensburgMadalena, Isabela RibeiroMarañón-Vásquez, Guido ArtemioOmori, Marjorie Ayumide Sousa, Emerson Tavaresda Silveira, Heitor Albergoni [UNESP]León, Jorge EsquicheBaratto-Filho, FlaresAlves, Sandra Yasuyo FukadaStuani, Maria Bernadete SassoNelson-Filho, PauloKirschneck, ChristianKüchler, Erika Calvano2023-07-29T13:24:59Z2023-07-29T13:24:59Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article345-352http://dx.doi.org/10.1007/s00784-022-04738-wClinical Oral Investigations, v. 27, n. 1, p. 345-352, 2023.1436-37711432-6981http://hdl.handle.net/11449/24775710.1007/s00784-022-04738-w2-s2.0-85140122114Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2023-07-29T13:24:59Zoai:repositorio.unesp.br:11449/247757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:07:46.354504Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
title |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
spellingShingle |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency Madalena, Isabela Ribeiro Estrogen receptor alpha Estrogen receptor beta Estrogens Odontogenesis Tooth eruption |
title_short |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
title_full |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
title_fullStr |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
title_full_unstemmed |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
title_sort |
Evaluation of tooth eruption rate of incisor teeth in rats with estrogen deficiency |
author |
Madalena, Isabela Ribeiro |
author_facet |
Madalena, Isabela Ribeiro Marañón-Vásquez, Guido Artemio Omori, Marjorie Ayumi de Sousa, Emerson Tavares da Silveira, Heitor Albergoni [UNESP] León, Jorge Esquiche Baratto-Filho, Flares Alves, Sandra Yasuyo Fukada Stuani, Maria Bernadete Sasso Nelson-Filho, Paulo Kirschneck, Christian Küchler, Erika Calvano |
author_role |
author |
author2 |
Marañón-Vásquez, Guido Artemio Omori, Marjorie Ayumi de Sousa, Emerson Tavares da Silveira, Heitor Albergoni [UNESP] León, Jorge Esquiche Baratto-Filho, Flares Alves, Sandra Yasuyo Fukada Stuani, Maria Bernadete Sasso Nelson-Filho, Paulo Kirschneck, Christian Küchler, Erika Calvano |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Cidade Universitária Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) University of the Joinville Region University Medical Centre of Regensburg |
dc.contributor.author.fl_str_mv |
Madalena, Isabela Ribeiro Marañón-Vásquez, Guido Artemio Omori, Marjorie Ayumi de Sousa, Emerson Tavares da Silveira, Heitor Albergoni [UNESP] León, Jorge Esquiche Baratto-Filho, Flares Alves, Sandra Yasuyo Fukada Stuani, Maria Bernadete Sasso Nelson-Filho, Paulo Kirschneck, Christian Küchler, Erika Calvano |
dc.subject.por.fl_str_mv |
Estrogen receptor alpha Estrogen receptor beta Estrogens Odontogenesis Tooth eruption |
topic |
Estrogen receptor alpha Estrogen receptor beta Estrogens Odontogenesis Tooth eruption |
description |
Objectives: To assess the influence of estrogen deficiency on tooth eruption rate (TER) and gene expression of estrogen receptor alpha and beta (ERα and ERβ) in the odontogenic region of teeth with continuous formation in a rat model. Materials and methods: Ovariectomies (OVX; n = 25) and sham surgeries (SHAM; n = 25) were performed in female Wistar rats when animals were 25 days old. The TER of the lower incisors, both in impeded (hyperfunction condition) and unimpeded (trimmed incisal edge—hypofunction condition) conditions, was evaluated using standardized digital photographs acquired every 48–72 h for 3 weeks (35th–53rd day of life), using a camera coupled to a stereomicroscope. Quantitative real-time PCR was performed to evaluate the relative gene expression of ERα and ERβ in the odontogenic region. Results: The OVX group showed a significant reduction in TER when compared to the SHAM group, only in the impeded condition (p = 0.03). There was no statistically significant difference between the groups in ERα gene expression (p = 0.33). ERβ showed a significantly higher gene expression in the OVX group (p ≤ 0.05). Conclusions: Estrogen deficiency decreases TER in teeth under impeded condition. Estrogen deficiency also increases ERβ gene expression in the odontogenic region of teeth with continuous formation. Clinical relevance: Hormonal disturbances affecting estrogen levels can cause alterations in dental formation and teeth eruption. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:24:59Z 2023-07-29T13:24:59Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-022-04738-w Clinical Oral Investigations, v. 27, n. 1, p. 345-352, 2023. 1436-3771 1432-6981 http://hdl.handle.net/11449/247757 10.1007/s00784-022-04738-w 2-s2.0-85140122114 |
url |
http://dx.doi.org/10.1007/s00784-022-04738-w http://hdl.handle.net/11449/247757 |
identifier_str_mv |
Clinical Oral Investigations, v. 27, n. 1, p. 345-352, 2023. 1436-3771 1432-6981 10.1007/s00784-022-04738-w 2-s2.0-85140122114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
345-352 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129394730860544 |