Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model

Detalhes bibliográficos
Autor(a) principal: Sarmiento-Machado, Luis Manuel [UNESP]
Data de Publicação: 2020
Outros Autores: Romualdo, Guilherme Ribeiro [UNESP], Zapaterini, Joyce Regina [UNESP], Tablas, Mariana Baptista [UNESP], Fernandes, Ana Angélica Henrique [UNESP], Moreno, Fernando Salvador, Barbisan, Luís Fernando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/01635581.2020.1764067
http://hdl.handle.net/11449/200428
Resumo: Capsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4–12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis.
id UNSP_e2bb3122f210dabb7cbaa3a8bbdaeedd
oai_identifier_str oai:repositorio.unesp.br:11449/200428
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat ModelCapsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4–12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis.Department of Morphology Biosciences Institute São Paulo State University (UNESP)Department Pathology Medical School São Paulo State University (UNESP)Department of Chemistry and Biochemistry Biosciences Institute São Paulo State University (UNESP)Department of Food and Experimental Nutrition Faculty of Pharmaceutical Sciences University of São Paulo (USP)Department of Morphology Biosciences Institute São Paulo State University (UNESP)Department Pathology Medical School São Paulo State University (UNESP)Department of Chemistry and Biochemistry Biosciences Institute São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Sarmiento-Machado, Luis Manuel [UNESP]Romualdo, Guilherme Ribeiro [UNESP]Zapaterini, Joyce Regina [UNESP]Tablas, Mariana Baptista [UNESP]Fernandes, Ana Angélica Henrique [UNESP]Moreno, Fernando SalvadorBarbisan, Luís Fernando [UNESP]2020-12-12T02:06:19Z2020-12-12T02:06:19Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/01635581.2020.1764067Nutrition and Cancer.1532-79140163-5581http://hdl.handle.net/11449/20042810.1080/01635581.2020.17640672-s2.0-85084855086Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNutrition and Cancerinfo:eu-repo/semantics/openAccess2024-09-03T13:14:32Zoai:repositorio.unesp.br:11449/200428Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
title Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
spellingShingle Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
Sarmiento-Machado, Luis Manuel [UNESP]
title_short Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
title_full Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
title_fullStr Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
title_full_unstemmed Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
title_sort Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model
author Sarmiento-Machado, Luis Manuel [UNESP]
author_facet Sarmiento-Machado, Luis Manuel [UNESP]
Romualdo, Guilherme Ribeiro [UNESP]
Zapaterini, Joyce Regina [UNESP]
Tablas, Mariana Baptista [UNESP]
Fernandes, Ana Angélica Henrique [UNESP]
Moreno, Fernando Salvador
Barbisan, Luís Fernando [UNESP]
author_role author
author2 Romualdo, Guilherme Ribeiro [UNESP]
Zapaterini, Joyce Regina [UNESP]
Tablas, Mariana Baptista [UNESP]
Fernandes, Ana Angélica Henrique [UNESP]
Moreno, Fernando Salvador
Barbisan, Luís Fernando [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Sarmiento-Machado, Luis Manuel [UNESP]
Romualdo, Guilherme Ribeiro [UNESP]
Zapaterini, Joyce Regina [UNESP]
Tablas, Mariana Baptista [UNESP]
Fernandes, Ana Angélica Henrique [UNESP]
Moreno, Fernando Salvador
Barbisan, Luís Fernando [UNESP]
description Capsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4–12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:06:19Z
2020-12-12T02:06:19Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/01635581.2020.1764067
Nutrition and Cancer.
1532-7914
0163-5581
http://hdl.handle.net/11449/200428
10.1080/01635581.2020.1764067
2-s2.0-85084855086
url http://dx.doi.org/10.1080/01635581.2020.1764067
http://hdl.handle.net/11449/200428
identifier_str_mv Nutrition and Cancer.
1532-7914
0163-5581
10.1080/01635581.2020.1764067
2-s2.0-85084855086
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nutrition and Cancer
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021366280224768