Postprandial lipemia causes oxidative stress in dogs
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2021.03.008 http://hdl.handle.net/11449/210339 |
Resumo: | Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TACFRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided. |
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Postprandial lipemia causes oxidative stress in dogsFeedAntioxidantsCaninePreanalyticalOxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TACFRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Londrina State Univ UEL, Dept Prevent Vet Med, Londrina, Parana, BrazilUniv Ctr Integrated Fac Ourinhos Unifio, Roque Quagliato Vet Hosp, Ourinhos, SP, BrazilSao Paulo State Univ, Coll Vet Med Aracatuba, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilUniv Ctr Integrated Fac Ourinhos Unifio, Dept Vet Med, Rodovia BR 153,Km 338, BR-19909100 Ourinhos, SP, BrazilSao Paulo State Univ, Coll Vet Med Aracatuba, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilFAPESP: 2018/18220-0FAPESP: 2018/16334-9Elsevier B.V.Universidade Estadual de Londrina (UEL)Univ Ctr Integrated Fac Ourinhos UnifioUniversidade Estadual Paulista (Unesp)Minucci Bonatto, Natalia CamilaOliveira, Paula Lima deMancebo, Aline MartinsCosta, Leticia RamosMelo Bosculo, Maria RaquelBosco, Anelise Maria [UNESP]Ciarlini, Paulo Cesar [UNESP]Floriano, Beatriz PerezBarros, Luiz Daniel deMartins de Almeida, Breno Fernando2021-06-25T15:05:23Z2021-06-25T15:05:23Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-286http://dx.doi.org/10.1016/j.rvsc.2021.03.008Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 136, p. 277-286, 2021.0034-5288http://hdl.handle.net/11449/21033910.1016/j.rvsc.2021.03.008WOS:000651455600012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch In Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-09-04T18:04:24Zoai:repositorio.unesp.br:11449/210339Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Postprandial lipemia causes oxidative stress in dogs |
title |
Postprandial lipemia causes oxidative stress in dogs |
spellingShingle |
Postprandial lipemia causes oxidative stress in dogs Minucci Bonatto, Natalia Camila Feed Antioxidants Canine Preanalytical |
title_short |
Postprandial lipemia causes oxidative stress in dogs |
title_full |
Postprandial lipemia causes oxidative stress in dogs |
title_fullStr |
Postprandial lipemia causes oxidative stress in dogs |
title_full_unstemmed |
Postprandial lipemia causes oxidative stress in dogs |
title_sort |
Postprandial lipemia causes oxidative stress in dogs |
author |
Minucci Bonatto, Natalia Camila |
author_facet |
Minucci Bonatto, Natalia Camila Oliveira, Paula Lima de Mancebo, Aline Martins Costa, Leticia Ramos Melo Bosculo, Maria Raquel Bosco, Anelise Maria [UNESP] Ciarlini, Paulo Cesar [UNESP] Floriano, Beatriz Perez Barros, Luiz Daniel de Martins de Almeida, Breno Fernando |
author_role |
author |
author2 |
Oliveira, Paula Lima de Mancebo, Aline Martins Costa, Leticia Ramos Melo Bosculo, Maria Raquel Bosco, Anelise Maria [UNESP] Ciarlini, Paulo Cesar [UNESP] Floriano, Beatriz Perez Barros, Luiz Daniel de Martins de Almeida, Breno Fernando |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Univ Ctr Integrated Fac Ourinhos Unifio Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Minucci Bonatto, Natalia Camila Oliveira, Paula Lima de Mancebo, Aline Martins Costa, Leticia Ramos Melo Bosculo, Maria Raquel Bosco, Anelise Maria [UNESP] Ciarlini, Paulo Cesar [UNESP] Floriano, Beatriz Perez Barros, Luiz Daniel de Martins de Almeida, Breno Fernando |
dc.subject.por.fl_str_mv |
Feed Antioxidants Canine Preanalytical |
topic |
Feed Antioxidants Canine Preanalytical |
description |
Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TACFRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:05:23Z 2021-06-25T15:05:23Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2021.03.008 Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 136, p. 277-286, 2021. 0034-5288 http://hdl.handle.net/11449/210339 10.1016/j.rvsc.2021.03.008 WOS:000651455600012 |
url |
http://dx.doi.org/10.1016/j.rvsc.2021.03.008 http://hdl.handle.net/11449/210339 |
identifier_str_mv |
Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 136, p. 277-286, 2021. 0034-5288 10.1016/j.rvsc.2021.03.008 WOS:000651455600012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research In Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
277-286 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021425441931264 |