Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572007000100007 http://hdl.handle.net/11449/30493 |
Resumo: | The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments were performed, it was demonstrated that: 1.) active NORs usually corresponded to segments with 18S rDNA genes identified in FISH experiments; 2.) several 18S rDNA sequences were not silver-stained, suggesting that they do not correspond to active NORs; and 3.) some chromosomes with silver-stained regions did not display any 18S rDNA signals. These findings characterize an extensive polymorphism associated with the NOR-bearing chromosomes of T. venezuelensis and emphasize the importance of combining traditional and molecular techniques in chromosome studies. |
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Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae)18S rDNAAg-NORsC-bandFISHfish cytogeneticssex chromosomesThe karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments were performed, it was demonstrated that: 1.) active NORs usually corresponded to segments with 18S rDNA genes identified in FISH experiments; 2.) several 18S rDNA sequences were not silver-stained, suggesting that they do not correspond to active NORs; and 3.) some chromosomes with silver-stained regions did not display any 18S rDNA signals. These findings characterize an extensive polymorphism associated with the NOR-bearing chromosomes of T. venezuelensis and emphasize the importance of combining traditional and molecular techniques in chromosome studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidad de Oriente Escuela de Ciencias Aplicadas del MarUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidad de Oriente Instituto LimnológicoUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaSociedade Brasileira de GenéticaUniversidad de OrienteUniversidade Estadual Paulista (Unesp)Universidad de Oriente Instituto LimnológicoNirchio, MauroOliveira, Claudio [UNESP]Ferreira, Irani Alves [UNESP]Granado, AngelRon, Ernesto2014-05-20T15:17:30Z2014-05-20T15:17:30Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25-30application/pdfhttp://dx.doi.org/10.1590/S1415-47572007000100007Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 1, p. 25-30, 2007.1415-4757http://hdl.handle.net/11449/3049310.1590/S1415-47572007000100007S1415-47572007000100007S1415-47572007000100007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-23T06:10:06Zoai:repositorio.unesp.br:11449/30493Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:45:40.238975Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
title |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
spellingShingle |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) Nirchio, Mauro 18S rDNA Ag-NORs C-band FISH fish cytogenetics sex chromosomes |
title_short |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
title_full |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
title_fullStr |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
title_full_unstemmed |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
title_sort |
Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae) |
author |
Nirchio, Mauro |
author_facet |
Nirchio, Mauro Oliveira, Claudio [UNESP] Ferreira, Irani Alves [UNESP] Granado, Angel Ron, Ernesto |
author_role |
author |
author2 |
Oliveira, Claudio [UNESP] Ferreira, Irani Alves [UNESP] Granado, Angel Ron, Ernesto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidad de Oriente Universidade Estadual Paulista (Unesp) Universidad de Oriente Instituto Limnológico |
dc.contributor.author.fl_str_mv |
Nirchio, Mauro Oliveira, Claudio [UNESP] Ferreira, Irani Alves [UNESP] Granado, Angel Ron, Ernesto |
dc.subject.por.fl_str_mv |
18S rDNA Ag-NORs C-band FISH fish cytogenetics sex chromosomes |
topic |
18S rDNA Ag-NORs C-band FISH fish cytogenetics sex chromosomes |
description |
The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments were performed, it was demonstrated that: 1.) active NORs usually corresponded to segments with 18S rDNA genes identified in FISH experiments; 2.) several 18S rDNA sequences were not silver-stained, suggesting that they do not correspond to active NORs; and 3.) some chromosomes with silver-stained regions did not display any 18S rDNA signals. These findings characterize an extensive polymorphism associated with the NOR-bearing chromosomes of T. venezuelensis and emphasize the importance of combining traditional and molecular techniques in chromosome studies. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:17:30Z 2014-05-20T15:17:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572007000100007 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 1, p. 25-30, 2007. 1415-4757 http://hdl.handle.net/11449/30493 10.1590/S1415-47572007000100007 S1415-47572007000100007 S1415-47572007000100007.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572007000100007 http://hdl.handle.net/11449/30493 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 1, p. 25-30, 2007. 1415-4757 10.1590/S1415-47572007000100007 S1415-47572007000100007 S1415-47572007000100007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25-30 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128558342602752 |