Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/9781216 http://hdl.handle.net/11449/178053 |
Resumo: | This work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC) and submerged (SmC) cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 � 0.8 U/g, and in SmC after 168 hours: 2,642 � 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 � 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 � 0.3 U/g after 240 h with 1: 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases. |
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Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged CultivationThis work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC) and submerged (SmC) cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 � 0.8 U/g, and in SmC after 168 hours: 2,642 � 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 � 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 � 0.3 U/g after 240 h with 1: 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laborat�rio de Bioqu�mica de Prote�nas Departamento de Qu�mica e Ci�ncias Ambientais Universidade Estadual Paulista (UNESP), Rua Cristov�o Colombo 2265Laborat�rio de Bioqu�mica e Microbiologia Aplicadas Departamento de Biologia Universidade Estadual Paulista (UNESP), Rua Cristov�o Colombo 2265Laborat�rio de Bioqu�mica de Prote�nas Departamento de Qu�mica e Ci�ncias Ambientais Universidade Estadual Paulista (UNESP), Rua Cristov�o Colombo 2265Laborat�rio de Bioqu�mica e Microbiologia Aplicadas Departamento de Biologia Universidade Estadual Paulista (UNESP), Rua Cristov�o Colombo 2265Universidade Estadual Paulista (Unesp)Teixeira Da Silva, Vanessa De C�ssia [UNESP]De Souza Coto, Amanda Lais [UNESP]De Carvalho Souza, Rafael [UNESP]Neves, Marcello Bertoldi Sanchez [UNESP]Gomes, Eleni [UNESP]Bonilla-Rodriguez, Gustavo Orlando [UNESP]2018-12-11T17:28:22Z2018-12-11T17:28:22Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/9781216Biochemistry Research International, v. 2016.2090-22552090-2247http://hdl.handle.net/11449/17805310.1155/2016/97812162-s2.0-849715391032-s2.0-84971539103.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemistry Research International0,437info:eu-repo/semantics/openAccess2024-01-21T06:22:13Zoai:repositorio.unesp.br:11449/178053Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:12.114390Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
title |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
spellingShingle |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation Teixeira Da Silva, Vanessa De C�ssia [UNESP] |
title_short |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
title_full |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
title_fullStr |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
title_full_unstemmed |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
title_sort |
Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation |
author |
Teixeira Da Silva, Vanessa De C�ssia [UNESP] |
author_facet |
Teixeira Da Silva, Vanessa De C�ssia [UNESP] De Souza Coto, Amanda Lais [UNESP] De Carvalho Souza, Rafael [UNESP] Neves, Marcello Bertoldi Sanchez [UNESP] Gomes, Eleni [UNESP] Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
author_role |
author |
author2 |
De Souza Coto, Amanda Lais [UNESP] De Carvalho Souza, Rafael [UNESP] Neves, Marcello Bertoldi Sanchez [UNESP] Gomes, Eleni [UNESP] Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Teixeira Da Silva, Vanessa De C�ssia [UNESP] De Souza Coto, Amanda Lais [UNESP] De Carvalho Souza, Rafael [UNESP] Neves, Marcello Bertoldi Sanchez [UNESP] Gomes, Eleni [UNESP] Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
description |
This work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC) and submerged (SmC) cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 � 0.8 U/g, and in SmC after 168 hours: 2,642 � 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 � 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 � 0.3 U/g after 240 h with 1: 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:28:22Z 2018-12-11T17:28:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/9781216 Biochemistry Research International, v. 2016. 2090-2255 2090-2247 http://hdl.handle.net/11449/178053 10.1155/2016/9781216 2-s2.0-84971539103 2-s2.0-84971539103.pdf |
url |
http://dx.doi.org/10.1155/2016/9781216 http://hdl.handle.net/11449/178053 |
identifier_str_mv |
Biochemistry Research International, v. 2016. 2090-2255 2090-2247 10.1155/2016/9781216 2-s2.0-84971539103 2-s2.0-84971539103.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemistry Research International 0,437 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129532811542528 |