Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tox.2023.153543 http://hdl.handle.net/11449/248932 |
Resumo: | This study aimed to evaluate the gold nanoparticles (AuNPs) animal sterilizing potential after intratesticular injections and long-term adverse reproductive and systemic effects. Adult male Wistar rats were divided into control and gold nanoparticle (AuNPs) groups. The rats received 200 µL of saline or AuNPs solution (16 µg/mL) on experimental days 1 and 7 (ED1 and ED7). After 150 days, the testicular blood flow was measured, and the rats were mated with females. After mating, male animals were euthanized for histological, cellular, and molecular evaluations. The female fertility indices and fetal development were also recorded. The results indicated increased blood flow in the testes of treated animals. Testes from treated rats had histological abnormalities, shorter seminiferous epithelia, and oxidative stress. Although the sperm concentration was lower in the AuNP-treated rats, there were no alterations in sperm morphology. Animals exposed to AuNPs had decreased male fertility indices, and their offspring had lighter and less efficient placentas. Additionally, the anogenital distance was longer in female fetuses. There were no changes in the histology of the kidney and liver, the lipid profile, and the serum levels of LH, testosterone, AST, ALT, ALP, albumin, and creatinine. The primary systemic effect was an increase in MDA levels in the liver and kidney, with only the liver experiencing an increase in CAT activity. In conclusion, AuNPs have a long-term impact on reproduction with very slight alterations in animal health. The development of reproductive biotechnologies that eliminate germ cells or treat local cancers can benefit from using AuNPs. |
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Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implicationsEpididymisNanotechnologyOxidative stressPlacentaSpermTestisThis study aimed to evaluate the gold nanoparticles (AuNPs) animal sterilizing potential after intratesticular injections and long-term adverse reproductive and systemic effects. Adult male Wistar rats were divided into control and gold nanoparticle (AuNPs) groups. The rats received 200 µL of saline or AuNPs solution (16 µg/mL) on experimental days 1 and 7 (ED1 and ED7). After 150 days, the testicular blood flow was measured, and the rats were mated with females. After mating, male animals were euthanized for histological, cellular, and molecular evaluations. The female fertility indices and fetal development were also recorded. The results indicated increased blood flow in the testes of treated animals. Testes from treated rats had histological abnormalities, shorter seminiferous epithelia, and oxidative stress. Although the sperm concentration was lower in the AuNP-treated rats, there were no alterations in sperm morphology. Animals exposed to AuNPs had decreased male fertility indices, and their offspring had lighter and less efficient placentas. Additionally, the anogenital distance was longer in female fetuses. There were no changes in the histology of the kidney and liver, the lipid profile, and the serum levels of LH, testosterone, AST, ALT, ALP, albumin, and creatinine. The primary systemic effect was an increase in MDA levels in the liver and kidney, with only the liver experiencing an increase in CAT activity. In conclusion, AuNPs have a long-term impact on reproduction with very slight alterations in animal health. The development of reproductive biotechnologies that eliminate germ cells or treat local cancers can benefit from using AuNPs.Laboratory of Cellular Biology Department of Morphology Institute of Biological Sciences Federal University of Minas Gerais MGLaboratory of Nanomaterials Nanobiomedical Research Group MGLaboratory of Endocrinology and Metabolism Department of Physiology and Pharmacology Institute of Biological Sciences Federal University of Minas Gerais MGDepartment of Physiology and Biophysics Institute of Biological Sciences Federal University of Minas Gerais MGLaboratory of Animal Reproduction and Toxicology Department of General Biology MGLaboratory of Experimental Research on Gynecology and Obstetrics Postgraduate Course of Tocogynecology Botucatu Medical School UnespClinical Hematology Laboratory Faculty of Pharmacy Biological Sciences Federal University of Minas Gerais MGLaboratory of Experimental Research on Gynecology and Obstetrics Postgraduate Course of Tocogynecology Botucatu Medical School UnespUniversidade Federal de Minas Gerais (UFMG)MGUniversidade Estadual Paulista (UNESP)Coimbra, John L PDantas, Graziela de P Fde Andrade, Lídia MBrener, Marcos R GViana, Pedro I MLopes, Roberta A.O G Gontijo, DanieleErvilha, Luiz O GAssis, Mirian Q.Barcelos, Luciola S.E Szawka, RaphaelDamasceno, Débora C [UNESP]Machado-Neves, MarianaMota, Ana P.Costa, Guilherme M J2023-07-29T13:57:41Z2023-07-29T13:57:41Z2023-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article153543http://dx.doi.org/10.1016/j.tox.2023.153543Toxicology, v. 492, p. 153543-.1879-3185http://hdl.handle.net/11449/24893210.1016/j.tox.2023.1535432-s2.0-85160877707Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicologyinfo:eu-repo/semantics/openAccess2024-08-16T14:06:43Zoai:repositorio.unesp.br:11449/248932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
title |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
spellingShingle |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications Coimbra, John L P Epididymis Nanotechnology Oxidative stress Placenta Sperm Testis |
title_short |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
title_full |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
title_fullStr |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
title_full_unstemmed |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
title_sort |
Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications |
author |
Coimbra, John L P |
author_facet |
Coimbra, John L P Dantas, Graziela de P F de Andrade, Lídia M Brener, Marcos R G Viana, Pedro I M Lopes, Roberta A. O G Gontijo, Daniele Ervilha, Luiz O G Assis, Mirian Q. Barcelos, Luciola S. E Szawka, Raphael Damasceno, Débora C [UNESP] Machado-Neves, Mariana Mota, Ana P. Costa, Guilherme M J |
author_role |
author |
author2 |
Dantas, Graziela de P F de Andrade, Lídia M Brener, Marcos R G Viana, Pedro I M Lopes, Roberta A. O G Gontijo, Daniele Ervilha, Luiz O G Assis, Mirian Q. Barcelos, Luciola S. E Szawka, Raphael Damasceno, Débora C [UNESP] Machado-Neves, Mariana Mota, Ana P. Costa, Guilherme M J |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) MG Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Coimbra, John L P Dantas, Graziela de P F de Andrade, Lídia M Brener, Marcos R G Viana, Pedro I M Lopes, Roberta A. O G Gontijo, Daniele Ervilha, Luiz O G Assis, Mirian Q. Barcelos, Luciola S. E Szawka, Raphael Damasceno, Débora C [UNESP] Machado-Neves, Mariana Mota, Ana P. Costa, Guilherme M J |
dc.subject.por.fl_str_mv |
Epididymis Nanotechnology Oxidative stress Placenta Sperm Testis |
topic |
Epididymis Nanotechnology Oxidative stress Placenta Sperm Testis |
description |
This study aimed to evaluate the gold nanoparticles (AuNPs) animal sterilizing potential after intratesticular injections and long-term adverse reproductive and systemic effects. Adult male Wistar rats were divided into control and gold nanoparticle (AuNPs) groups. The rats received 200 µL of saline or AuNPs solution (16 µg/mL) on experimental days 1 and 7 (ED1 and ED7). After 150 days, the testicular blood flow was measured, and the rats were mated with females. After mating, male animals were euthanized for histological, cellular, and molecular evaluations. The female fertility indices and fetal development were also recorded. The results indicated increased blood flow in the testes of treated animals. Testes from treated rats had histological abnormalities, shorter seminiferous epithelia, and oxidative stress. Although the sperm concentration was lower in the AuNP-treated rats, there were no alterations in sperm morphology. Animals exposed to AuNPs had decreased male fertility indices, and their offspring had lighter and less efficient placentas. Additionally, the anogenital distance was longer in female fetuses. There were no changes in the histology of the kidney and liver, the lipid profile, and the serum levels of LH, testosterone, AST, ALT, ALP, albumin, and creatinine. The primary systemic effect was an increase in MDA levels in the liver and kidney, with only the liver experiencing an increase in CAT activity. In conclusion, AuNPs have a long-term impact on reproduction with very slight alterations in animal health. The development of reproductive biotechnologies that eliminate germ cells or treat local cancers can benefit from using AuNPs. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:57:41Z 2023-07-29T13:57:41Z 2023-06-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tox.2023.153543 Toxicology, v. 492, p. 153543-. 1879-3185 http://hdl.handle.net/11449/248932 10.1016/j.tox.2023.153543 2-s2.0-85160877707 |
url |
http://dx.doi.org/10.1016/j.tox.2023.153543 http://hdl.handle.net/11449/248932 |
identifier_str_mv |
Toxicology, v. 492, p. 153543-. 1879-3185 10.1016/j.tox.2023.153543 2-s2.0-85160877707 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
153543 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128125139156992 |