Avaliação da citotoxicidade e potencial antimicrobiano/antibiofilme de ácidos fenólicos sobre microrganismos relacionados com infecções endodônticas.

• Autor(a) principal: Oliveira, Warlley Campos de [UNESP]
• Data de Publicação: 2020
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/213894
• topic: Fibroblasts; Fenóis; Citotoxicidade; Fibroblastos; Anti-infecciosos

The antimicrobial action must be considered a fundamental property of the substances that are used during endodontic treatment. However, even with the reduction of the microbiota present inside the root canals after conventional chemical-mechanical treatment, the permanence of microorganisms due to the anatomical complexity of the root canals system and their resistance to treatment can cause persistent or secondary infections. Polyphenols are secondary metabolites produced by large plants that have therapeutic potential for the human body, mainly as antimicrobial, antioxidant, antiallergic, anti-inflammatory, anti-cancer, anti-hypertensive agents. Among these polyphenols, phenolic acids such as cinnamic acids and their derivatives, gallic acid and vanillic acid have been highlighted by their antibacterial and antifungal effects. The aim of this study was to evaluate the antimicrobial activity of the following phenolic acids: cinnamic acid and its derivative caffeic acid, gallic acid and vanillic acid on microorganisms related to endodontic infections, in addition to the cytotoxic potential on fibroblasts. The antimicrobial activity was evaluated by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on Enterococcus faecalis, Streptococcus mutans, Actinomyces israelii and Lactobacillus casei. The toxicity of phenolic acids was also assessed on fibroblasts of the L-929 strain, using the resazurin assay. The data were analyzed statistically, considering p <0.05. All phenolic acids tested showed an inhibitory effect for at least one of the bacterial species tested with MIC values between 0.25 to 2 mg/mL, with the most prominent acids being caffeic acid and cinnamic acid. For the viability of fibroblasts, cinnamic acid did not show a cytotoxic effect from 0.5 mg mL for times of exposure to treatments of 24 h and 48 h. Caffeic acid, on the other hand, showed cytocompatibility in concentrations from 0.125 mg/mL and 0.062 mg/mL for 24 h and 48 h respectively. These findings revealed that, cinnamic acid demonstrated potential as active principle alternative to antimicrobial for the treatment of endodontic infections.