Influence of artificial saliva in biofilm formation of Candida albicans in vitro
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242012000100005 http://hdl.handle.net/11449/22677 |
Resumo: | Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p <= 0.05). |
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Repositório Institucional da UNESP |
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Influence of artificial saliva in biofilm formation of Candida albicans in vitroSaliva, ArtificialCandida albicansBiofilmDue to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p <= 0.05).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, BrazilUNESP Univ Estadual Paulista, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, BrazilFAPESP: 10/14434-4Sociedade Brasileira de Pesquisa Odontológica (SBPqO)Universidade Estadual Paulista (Unesp)Silva, Michelle Peneluppi [UNESP]Chibebe Junior, Jose [UNESP]Jorjao, Adeline Lacerda [UNESP]da Silva Machado, Ana Karina [UNESP]de Oliveira, Luciane Dias [UNESP]Junqueira, Juliana Campos [UNESP]Cardoso Jorge, Antonio Olavo [UNESP]2014-05-20T14:04:38Z2014-05-20T14:04:38Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24-28application/pdfhttp://dx.doi.org/10.1590/S1806-83242012000100005Brazilian Oral Research. São Paulo: Sociedade Brasileira de Pesquisa Odontologica, v. 26, n. 1, p. 24-28, 2012.1806-8324http://hdl.handle.net/11449/2267710.1590/S1806-83242012000100005S1806-83242012000100005WOS:000311862300005S1806-83242012000100005.pdf471667661133031103220205410559000053567153623569Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-01-19T06:33:33Zoai:repositorio.unesp.br:11449/22677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:26:01.417711Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
title |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
spellingShingle |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro Silva, Michelle Peneluppi [UNESP] Saliva, Artificial Candida albicans Biofilm |
title_short |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
title_full |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
title_fullStr |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
title_full_unstemmed |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
title_sort |
Influence of artificial saliva in biofilm formation of Candida albicans in vitro |
author |
Silva, Michelle Peneluppi [UNESP] |
author_facet |
Silva, Michelle Peneluppi [UNESP] Chibebe Junior, Jose [UNESP] Jorjao, Adeline Lacerda [UNESP] da Silva Machado, Ana Karina [UNESP] de Oliveira, Luciane Dias [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
author_role |
author |
author2 |
Chibebe Junior, Jose [UNESP] Jorjao, Adeline Lacerda [UNESP] da Silva Machado, Ana Karina [UNESP] de Oliveira, Luciane Dias [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Michelle Peneluppi [UNESP] Chibebe Junior, Jose [UNESP] Jorjao, Adeline Lacerda [UNESP] da Silva Machado, Ana Karina [UNESP] de Oliveira, Luciane Dias [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
dc.subject.por.fl_str_mv |
Saliva, Artificial Candida albicans Biofilm |
topic |
Saliva, Artificial Candida albicans Biofilm |
description |
Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p <= 0.05). |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T14:04:38Z 2014-05-20T14:04:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242012000100005 Brazilian Oral Research. São Paulo: Sociedade Brasileira de Pesquisa Odontologica, v. 26, n. 1, p. 24-28, 2012. 1806-8324 http://hdl.handle.net/11449/22677 10.1590/S1806-83242012000100005 S1806-83242012000100005 WOS:000311862300005 S1806-83242012000100005.pdf 4716676611330311 0322020541055900 0053567153623569 |
url |
http://dx.doi.org/10.1590/S1806-83242012000100005 http://hdl.handle.net/11449/22677 |
identifier_str_mv |
Brazilian Oral Research. São Paulo: Sociedade Brasileira de Pesquisa Odontologica, v. 26, n. 1, p. 24-28, 2012. 1806-8324 10.1590/S1806-83242012000100005 S1806-83242012000100005 WOS:000311862300005 S1806-83242012000100005.pdf 4716676611330311 0322020541055900 0053567153623569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
24-28 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129520137404416 |