Investigation on the interaction between nanocarriers and functional compounds of flavonoids
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.17660/ActaHortic.2018.1198.17 http://hdl.handle.net/11449/185696 |
Resumo: | Studies have suggested that a diet rich in functional foods containing flavonoids provides benefits to human health for anti-inflammatory, antioxidant, antibacterial, and anticancer activities. These compounds are usually present in fruits, vegetables and beverages. Human Serum Albumin (HSA) is a predominant carrier protein in blood plasma. The exceptional capacity of HSA to interact with many molecules makes this protein an important regulator of intercellular fluxes. In this work, HSA was used as a model for the study of nanocarrier-flavonoid interaction. The flavonoids investigated were Rutin (RU) and Guaijaverin (GUA). For analytical studies a combination of steady-state fluorescence spectroscopy and molecular modelling calculations were employed. The fluorescence quenching results indicated that the flavonoids binding near residue Trp214, which is localized in the subdomain IIA of HSA. The quenching mechanism is static which indicates the formation of the HSA-flavonoid complexes. The Gibbs free energy changes of both complexes were negative which characterize spontaneous reactions. The molecular modelling results for binding of RU and GUA with HSA are in accordance with the fluorescence spectroscopy data. |
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Repositório Institucional da UNESP |
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2946 |
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Investigation on the interaction between nanocarriers and functional compounds of flavonoidsflavonoidsfluorescence spectroscopymolecular modellingnanocarrierStudies have suggested that a diet rich in functional foods containing flavonoids provides benefits to human health for anti-inflammatory, antioxidant, antibacterial, and anticancer activities. These compounds are usually present in fruits, vegetables and beverages. Human Serum Albumin (HSA) is a predominant carrier protein in blood plasma. The exceptional capacity of HSA to interact with many molecules makes this protein an important regulator of intercellular fluxes. In this work, HSA was used as a model for the study of nanocarrier-flavonoid interaction. The flavonoids investigated were Rutin (RU) and Guaijaverin (GUA). For analytical studies a combination of steady-state fluorescence spectroscopy and molecular modelling calculations were employed. The fluorescence quenching results indicated that the flavonoids binding near residue Trp214, which is localized in the subdomain IIA of HSA. The quenching mechanism is static which indicates the formation of the HSA-flavonoid complexes. The Gibbs free energy changes of both complexes were negative which characterize spontaneous reactions. The molecular modelling results for binding of RU and GUA with HSA are in accordance with the fluorescence spectroscopy data.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUNESP, Inst Chem, IQ, BR-14800900 Araraquara, SP, BrazilUNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUNESP, Inst Chem, IQ, BR-14800900 Araraquara, SP, BrazilInt Soc Horticultural ScienceUniversidade Estadual Paulista (Unesp)Caruso, I. P. [UNESP]Vilegas, W. [UNESP]Souza, F. P. [UNESP]Fossey, M. A. [UNESP]Cornelio, M. L. [UNESP]Narain, N.2019-10-04T12:37:48Z2019-10-04T12:37:48Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject93-96http://dx.doi.org/10.17660/ActaHortic.2018.1198.17Iii International Symposium On Medicinal And Nutraceutical Plants And Iii Conference Of National Institute Of Science And Technology For Tropical Fruits. Leuven 1: Int Soc Horticultural Science, v. 1198, p. 93-96, 2018.0567-7572http://hdl.handle.net/11449/18569610.17660/ActaHortic.2018.1198.17WOS:000467240500017Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIii International Symposium On Medicinal And Nutraceutical Plants And Iii Conference Of National Institute Of Science And Technology For Tropical Fruitsinfo:eu-repo/semantics/openAccess2021-10-23T02:05:29Zoai:repositorio.unesp.br:11449/185696Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:51:17.932134Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
title |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
spellingShingle |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids Caruso, I. P. [UNESP] flavonoids fluorescence spectroscopy molecular modelling nanocarrier |
title_short |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
title_full |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
title_fullStr |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
title_full_unstemmed |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
title_sort |
Investigation on the interaction between nanocarriers and functional compounds of flavonoids |
author |
Caruso, I. P. [UNESP] |
author_facet |
Caruso, I. P. [UNESP] Vilegas, W. [UNESP] Souza, F. P. [UNESP] Fossey, M. A. [UNESP] Cornelio, M. L. [UNESP] Narain, N. |
author_role |
author |
author2 |
Vilegas, W. [UNESP] Souza, F. P. [UNESP] Fossey, M. A. [UNESP] Cornelio, M. L. [UNESP] Narain, N. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Caruso, I. P. [UNESP] Vilegas, W. [UNESP] Souza, F. P. [UNESP] Fossey, M. A. [UNESP] Cornelio, M. L. [UNESP] Narain, N. |
dc.subject.por.fl_str_mv |
flavonoids fluorescence spectroscopy molecular modelling nanocarrier |
topic |
flavonoids fluorescence spectroscopy molecular modelling nanocarrier |
description |
Studies have suggested that a diet rich in functional foods containing flavonoids provides benefits to human health for anti-inflammatory, antioxidant, antibacterial, and anticancer activities. These compounds are usually present in fruits, vegetables and beverages. Human Serum Albumin (HSA) is a predominant carrier protein in blood plasma. The exceptional capacity of HSA to interact with many molecules makes this protein an important regulator of intercellular fluxes. In this work, HSA was used as a model for the study of nanocarrier-flavonoid interaction. The flavonoids investigated were Rutin (RU) and Guaijaverin (GUA). For analytical studies a combination of steady-state fluorescence spectroscopy and molecular modelling calculations were employed. The fluorescence quenching results indicated that the flavonoids binding near residue Trp214, which is localized in the subdomain IIA of HSA. The quenching mechanism is static which indicates the formation of the HSA-flavonoid complexes. The Gibbs free energy changes of both complexes were negative which characterize spontaneous reactions. The molecular modelling results for binding of RU and GUA with HSA are in accordance with the fluorescence spectroscopy data. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2019-10-04T12:37:48Z 2019-10-04T12:37:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.17660/ActaHortic.2018.1198.17 Iii International Symposium On Medicinal And Nutraceutical Plants And Iii Conference Of National Institute Of Science And Technology For Tropical Fruits. Leuven 1: Int Soc Horticultural Science, v. 1198, p. 93-96, 2018. 0567-7572 http://hdl.handle.net/11449/185696 10.17660/ActaHortic.2018.1198.17 WOS:000467240500017 |
url |
http://dx.doi.org/10.17660/ActaHortic.2018.1198.17 http://hdl.handle.net/11449/185696 |
identifier_str_mv |
Iii International Symposium On Medicinal And Nutraceutical Plants And Iii Conference Of National Institute Of Science And Technology For Tropical Fruits. Leuven 1: Int Soc Horticultural Science, v. 1198, p. 93-96, 2018. 0567-7572 10.17660/ActaHortic.2018.1198.17 WOS:000467240500017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Iii International Symposium On Medicinal And Nutraceutical Plants And Iii Conference Of National Institute Of Science And Technology For Tropical Fruits |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-96 |
dc.publisher.none.fl_str_mv |
Int Soc Horticultural Science |
publisher.none.fl_str_mv |
Int Soc Horticultural Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128425497460736 |