Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0225895 http://hdl.handle.net/11449/197780 |
Resumo: | Background Arboviruses and protozoans can cause neurologic disorders in horses. In Brazilian Amazon, several horses presenting signs compatible with disorders caused by these infectious agents have been observed. Objective To contribute to the knowledge of this epidemiological picture, we sought to construct a serological diagnostic panel for neurotrophic infectious agents in local horses. Material and methods A total of 213 blood samples from horses were collected from 29 farms in three municipalities. Samples were evaluated and considered positive when they met the following criteria: titers >= 1:80 with the indirect fluorescent antibody test (IFAT) for apicomplexan protozoans; positive recombinant enzyme-linked immunosorbent assay (ELISA) with subsequent titers >= 1:10 by the PRNt for viruses; and detection under direct microscopic examination for Trypanosoma evansi. Results No horses were found to be infected by T. evansi, and only two were infected Toxoplasma gondii and/or Neospora spp. The highest protozoan infection rate was observed for Sarcocystis neurona (40.3%; n = 86/213). Among the positive ELISA samples tested by the plaque reduction neutralization test (PRNT90), 92% (n = 76/83) were positive for St Louis Encephalitis virus, 43% (n = 6/14) were positive for West Nile virus and 33% (n = 16/48) were positive for Mayaro virus. Eighteen percent (n = 39/213) of horses were co-infected by S. neurona and at least one arbovirus, particularly SLEV and/or MAYV. Conclusion Samples positive for SLEV associated with S. neurona, including samples from horses that had recovered from neurological signs were frequent, and must be considered when investigating the possible causes of neurological diseases in South Roraima horses. |
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Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of BrazilBackground Arboviruses and protozoans can cause neurologic disorders in horses. In Brazilian Amazon, several horses presenting signs compatible with disorders caused by these infectious agents have been observed. Objective To contribute to the knowledge of this epidemiological picture, we sought to construct a serological diagnostic panel for neurotrophic infectious agents in local horses. Material and methods A total of 213 blood samples from horses were collected from 29 farms in three municipalities. Samples were evaluated and considered positive when they met the following criteria: titers >= 1:80 with the indirect fluorescent antibody test (IFAT) for apicomplexan protozoans; positive recombinant enzyme-linked immunosorbent assay (ELISA) with subsequent titers >= 1:10 by the PRNt for viruses; and detection under direct microscopic examination for Trypanosoma evansi. Results No horses were found to be infected by T. evansi, and only two were infected Toxoplasma gondii and/or Neospora spp. The highest protozoan infection rate was observed for Sarcocystis neurona (40.3%; n = 86/213). Among the positive ELISA samples tested by the plaque reduction neutralization test (PRNT90), 92% (n = 76/83) were positive for St Louis Encephalitis virus, 43% (n = 6/14) were positive for West Nile virus and 33% (n = 16/48) were positive for Mayaro virus. Eighteen percent (n = 39/213) of horses were co-infected by S. neurona and at least one arbovirus, particularly SLEV and/or MAYV. Conclusion Samples positive for SLEV associated with S. neurona, including samples from horses that had recovered from neurological signs were frequent, and must be considered when investigating the possible causes of neurological diseases in South Roraima horses.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fiocruz MS, Oswaldo Cruz Inst, Lab Trypanosomatid Biol, Rio De Janeiro, RJ, BrazilFed Inst Educ Sci & Technol Roraima, Caracarai, Roraima, BrazilSao Paulo State Univ, Sch Agr & Vet Studies Jaboticabal, Jaboticabal, SP, BrazilUniv Sao Paulo, Sch Vet & Anim Sci, Dept Prevent Vet & Anim Hlth, Sao Paulo, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Virol Res Ctr, Ribeirao Preto, BrazilSao Paulo State Univ, Sch Agr & Vet Studies Jaboticabal, Jaboticabal, SP, BrazilCAPES: 001Public Library ScienceFiocruz MSFed Inst Educ Sci & Technol RoraimaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Gomes, Fabio AlvesJansen, Ana MariaMachado, Rosangela Zacarias [UNESP]Jesus Pena, Hilda FatimaFumagalli, Marcilio JorgeSilva, AngelicaAlves, Bruna FariasRodrigues Roque, Andre LuizMoraes Figueiredo, Luiz Tadeu2020-12-11T17:20:22Z2020-12-11T17:20:22Z2019-12-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.1371/journal.pone.0225895Plos One. San Francisco: Public Library Science, v. 14, n. 12, 13 p., 2019.1932-6203http://hdl.handle.net/11449/19778010.1371/journal.pone.0225895WOS:000534070200033Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2024-06-07T13:02:06Zoai:repositorio.unesp.br:11449/197780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:48.550085Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
title |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
spellingShingle |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil Gomes, Fabio Alves |
title_short |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
title_full |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
title_fullStr |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
title_full_unstemmed |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
title_sort |
Serological evidence of arboviruses and coccidia infecting horses in the Amazonian region of Brazil |
author |
Gomes, Fabio Alves |
author_facet |
Gomes, Fabio Alves Jansen, Ana Maria Machado, Rosangela Zacarias [UNESP] Jesus Pena, Hilda Fatima Fumagalli, Marcilio Jorge Silva, Angelica Alves, Bruna Farias Rodrigues Roque, Andre Luiz Moraes Figueiredo, Luiz Tadeu |
author_role |
author |
author2 |
Jansen, Ana Maria Machado, Rosangela Zacarias [UNESP] Jesus Pena, Hilda Fatima Fumagalli, Marcilio Jorge Silva, Angelica Alves, Bruna Farias Rodrigues Roque, Andre Luiz Moraes Figueiredo, Luiz Tadeu |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fiocruz MS Fed Inst Educ Sci & Technol Roraima Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gomes, Fabio Alves Jansen, Ana Maria Machado, Rosangela Zacarias [UNESP] Jesus Pena, Hilda Fatima Fumagalli, Marcilio Jorge Silva, Angelica Alves, Bruna Farias Rodrigues Roque, Andre Luiz Moraes Figueiredo, Luiz Tadeu |
description |
Background Arboviruses and protozoans can cause neurologic disorders in horses. In Brazilian Amazon, several horses presenting signs compatible with disorders caused by these infectious agents have been observed. Objective To contribute to the knowledge of this epidemiological picture, we sought to construct a serological diagnostic panel for neurotrophic infectious agents in local horses. Material and methods A total of 213 blood samples from horses were collected from 29 farms in three municipalities. Samples were evaluated and considered positive when they met the following criteria: titers >= 1:80 with the indirect fluorescent antibody test (IFAT) for apicomplexan protozoans; positive recombinant enzyme-linked immunosorbent assay (ELISA) with subsequent titers >= 1:10 by the PRNt for viruses; and detection under direct microscopic examination for Trypanosoma evansi. Results No horses were found to be infected by T. evansi, and only two were infected Toxoplasma gondii and/or Neospora spp. The highest protozoan infection rate was observed for Sarcocystis neurona (40.3%; n = 86/213). Among the positive ELISA samples tested by the plaque reduction neutralization test (PRNT90), 92% (n = 76/83) were positive for St Louis Encephalitis virus, 43% (n = 6/14) were positive for West Nile virus and 33% (n = 16/48) were positive for Mayaro virus. Eighteen percent (n = 39/213) of horses were co-infected by S. neurona and at least one arbovirus, particularly SLEV and/or MAYV. Conclusion Samples positive for SLEV associated with S. neurona, including samples from horses that had recovered from neurological signs were frequent, and must be considered when investigating the possible causes of neurological diseases in South Roraima horses. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-12 2020-12-11T17:20:22Z 2020-12-11T17:20:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0225895 Plos One. San Francisco: Public Library Science, v. 14, n. 12, 13 p., 2019. 1932-6203 http://hdl.handle.net/11449/197780 10.1371/journal.pone.0225895 WOS:000534070200033 |
url |
http://dx.doi.org/10.1371/journal.pone.0225895 http://hdl.handle.net/11449/197780 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 14, n. 12, 13 p., 2019. 1932-6203 10.1371/journal.pone.0225895 WOS:000534070200033 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Plos One |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128794581532672 |