Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality

Detalhes bibliográficos
Autor(a) principal: Bagagli, E. [UNESP]
Data de Publicação: 1995
Outros Autores: Furlaneto, M. C. [UNESP], Pizzirani-Kleiner, A. [UNESP], Azevedo, J. L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1139/m95-157
http://hdl.handle.net/11449/224013
Resumo: Heterokaryons were obtained by hyphal anastomosis in Trichoderma pseudokoningii using strains with morphologic and double auxotrophic genetic markers. Stable haploid and unstable hyperhaploid recombinants were detected in monosporic colonies derived from heterokaryons and selected in appropriate media or by an enrichment filtration technique. Diploids were not observed and the recombinant frequencies were higher than those found in fungi with a typical parasexual cycle. The mechanism of recombinant emergence may involve a transient diploid formation followed by chromosomal losses at the hyphal stages, resulting in haploid and hyperhaploid recombinants. The possibility of intrachromosomal rearrangement with the involvement of cytoplasmatic DNA as a heterokaryotic transformation system cannot be excluded.
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spelling Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexualityEvolutionParasexual processTransformationTrichoderma pseudokoningiiHeterokaryons were obtained by hyphal anastomosis in Trichoderma pseudokoningii using strains with morphologic and double auxotrophic genetic markers. Stable haploid and unstable hyperhaploid recombinants were detected in monosporic colonies derived from heterokaryons and selected in appropriate media or by an enrichment filtration technique. Diploids were not observed and the recombinant frequencies were higher than those found in fungi with a typical parasexual cycle. The mechanism of recombinant emergence may involve a transient diploid formation followed by chromosomal losses at the hyphal stages, resulting in haploid and hyperhaploid recombinants. The possibility of intrachromosomal rearrangement with the involvement of cytoplasmatic DNA as a heterokaryotic transformation system cannot be excluded.Dept. Microbiology and Immunology Institute of Biosciences Sao Paulo State University, 18618-000 Botucatu, Sao PauloDept. Microbiology and Immunology Institute of Biosciences Sao Paulo State University, 18618-000 Botucatu, Sao PauloUniversidade Estadual Paulista (UNESP)Bagagli, E. [UNESP]Furlaneto, M. C. [UNESP]Pizzirani-Kleiner, A. [UNESP]Azevedo, J. L. [UNESP]2022-04-28T19:54:19Z2022-04-28T19:54:19Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1132-1134http://dx.doi.org/10.1139/m95-157Canadian Journal of Microbiology, v. 41, n. 12, p. 1132-1134, 1995.0008-4166http://hdl.handle.net/11449/22401310.1139/m95-1572-s2.0-0029616477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T19:54:19Zoai:repositorio.unesp.br:11449/224013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:27:02.759816Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
title Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
spellingShingle Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
Bagagli, E. [UNESP]
Evolution
Parasexual process
Transformation
Trichoderma pseudokoningii
title_short Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
title_full Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
title_fullStr Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
title_full_unstemmed Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
title_sort Genetic recombinants in Trichoderma pseudokoningii (Rifai) without typical parasexuality
author Bagagli, E. [UNESP]
author_facet Bagagli, E. [UNESP]
Furlaneto, M. C. [UNESP]
Pizzirani-Kleiner, A. [UNESP]
Azevedo, J. L. [UNESP]
author_role author
author2 Furlaneto, M. C. [UNESP]
Pizzirani-Kleiner, A. [UNESP]
Azevedo, J. L. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Bagagli, E. [UNESP]
Furlaneto, M. C. [UNESP]
Pizzirani-Kleiner, A. [UNESP]
Azevedo, J. L. [UNESP]
dc.subject.por.fl_str_mv Evolution
Parasexual process
Transformation
Trichoderma pseudokoningii
topic Evolution
Parasexual process
Transformation
Trichoderma pseudokoningii
description Heterokaryons were obtained by hyphal anastomosis in Trichoderma pseudokoningii using strains with morphologic and double auxotrophic genetic markers. Stable haploid and unstable hyperhaploid recombinants were detected in monosporic colonies derived from heterokaryons and selected in appropriate media or by an enrichment filtration technique. Diploids were not observed and the recombinant frequencies were higher than those found in fungi with a typical parasexual cycle. The mechanism of recombinant emergence may involve a transient diploid formation followed by chromosomal losses at the hyphal stages, resulting in haploid and hyperhaploid recombinants. The possibility of intrachromosomal rearrangement with the involvement of cytoplasmatic DNA as a heterokaryotic transformation system cannot be excluded.
publishDate 1995
dc.date.none.fl_str_mv 1995-01-01
2022-04-28T19:54:19Z
2022-04-28T19:54:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1139/m95-157
Canadian Journal of Microbiology, v. 41, n. 12, p. 1132-1134, 1995.
0008-4166
http://hdl.handle.net/11449/224013
10.1139/m95-157
2-s2.0-0029616477
url http://dx.doi.org/10.1139/m95-157
http://hdl.handle.net/11449/224013
identifier_str_mv Canadian Journal of Microbiology, v. 41, n. 12, p. 1132-1134, 1995.
0008-4166
10.1139/m95-157
2-s2.0-0029616477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canadian Journal of Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1132-1134
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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