Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/03639045.2020.1716374 http://hdl.handle.net/11449/201495 |
Resumo: | Nanoparticles based on gellan gum/pectin blends were designed for colon-targeted release of resveratrol (RES). Their impact on drug release rates and permeability were evaluated using Caco-2 cell model and mucus secreting triple co-culture model. Polymeric nanoparticles (PNP) were successfully prepared by nebulization/ionotropic gelation, achieving high drug loading (>80%). PNP were spherical with a low positive charge density (+5mV) and exhibited diameters of around 330 nm. Developed PNP were able to promote effective modulation of drug release rates, so that only 3% of RES was released in acidic media over 2 h, and, in pH 6.8, the drug was released in a sustained manner, reaching 85% in 30 h. The permeability of RES-loaded PNP in the Caco-2 model was 0.15%, while in the triple co-culture model, in the presence of mucus, it reached 5.5%. The everted gut sac experiment corroborated the low permeability of RES-loaded PNP in the presence or absence of mucus and highlighted their high ability to interact with the intestinal tissue. Results indicate that the novel PNP developed in this work are safe and promising carriers for controlled delivery of RES at the colon. |
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Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrolcaco-2 cellscolon-targeted releaseeverted gut sacin vitro permeabilityPolymeric nanoparticlestriple co-culture modelNanoparticles based on gellan gum/pectin blends were designed for colon-targeted release of resveratrol (RES). Their impact on drug release rates and permeability were evaluated using Caco-2 cell model and mucus secreting triple co-culture model. Polymeric nanoparticles (PNP) were successfully prepared by nebulization/ionotropic gelation, achieving high drug loading (>80%). PNP were spherical with a low positive charge density (+5mV) and exhibited diameters of around 330 nm. Developed PNP were able to promote effective modulation of drug release rates, so that only 3% of RES was released in acidic media over 2 h, and, in pH 6.8, the drug was released in a sustained manner, reaching 85% in 30 h. The permeability of RES-loaded PNP in the Caco-2 model was 0.15%, while in the triple co-culture model, in the presence of mucus, it reached 5.5%. The everted gut sac experiment corroborated the low permeability of RES-loaded PNP in the presence or absence of mucus and highlighted their high ability to interact with the intestinal tissue. Results indicate that the novel PNP developed in this work are safe and promising carriers for controlled delivery of RES at the colon.School of Pharmaceutical Sciences São Paulo State University (UNESP)Chemistry Institute of São Carlos São Paulo University (USP)I3S-Instituto de Investigação e Inovação em Saúde Universidade do PortoINEB-Instituto de Engenharia Biomédica Universidade do PortoCESPU-Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da SaúdeSchool of Pharmaceutical Sciences São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade do PortoCESPU-Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da SaúdePrezotti, Fabíola Garavello [UNESP]Boni, Fernanda Isadora [UNESP]Ferreira, Natália Noronha [UNESP]Silva, Daniella SouzaAlmeida, AndreiaVasconcelos, TeófiloSarmento, BrunoGremião, Maria Palmira Daflon [UNESP]Cury, Beatriz Stringhetti Ferreira [UNESP]2020-12-12T02:34:00Z2020-12-12T02:34:00Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article236-245http://dx.doi.org/10.1080/03639045.2020.1716374Drug Development and Industrial Pharmacy, v. 46, n. 2, p. 236-245, 2020.1520-57620363-9045http://hdl.handle.net/11449/20149510.1080/03639045.2020.17163742-s2.0-85078402703Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDrug Development and Industrial Pharmacyinfo:eu-repo/semantics/openAccess2024-06-24T13:45:18Zoai:repositorio.unesp.br:11449/201495Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:59:08.768524Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
title |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
spellingShingle |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol Prezotti, Fabíola Garavello [UNESP] caco-2 cells colon-targeted release everted gut sac in vitro permeability Polymeric nanoparticles triple co-culture model |
title_short |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
title_full |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
title_fullStr |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
title_full_unstemmed |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
title_sort |
Oral nanoparticles based on gellan gum/pectin for colon-targeted delivery of resveratrol |
author |
Prezotti, Fabíola Garavello [UNESP] |
author_facet |
Prezotti, Fabíola Garavello [UNESP] Boni, Fernanda Isadora [UNESP] Ferreira, Natália Noronha [UNESP] Silva, Daniella Souza Almeida, Andreia Vasconcelos, Teófilo Sarmento, Bruno Gremião, Maria Palmira Daflon [UNESP] Cury, Beatriz Stringhetti Ferreira [UNESP] |
author_role |
author |
author2 |
Boni, Fernanda Isadora [UNESP] Ferreira, Natália Noronha [UNESP] Silva, Daniella Souza Almeida, Andreia Vasconcelos, Teófilo Sarmento, Bruno Gremião, Maria Palmira Daflon [UNESP] Cury, Beatriz Stringhetti Ferreira [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade do Porto CESPU-Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde |
dc.contributor.author.fl_str_mv |
Prezotti, Fabíola Garavello [UNESP] Boni, Fernanda Isadora [UNESP] Ferreira, Natália Noronha [UNESP] Silva, Daniella Souza Almeida, Andreia Vasconcelos, Teófilo Sarmento, Bruno Gremião, Maria Palmira Daflon [UNESP] Cury, Beatriz Stringhetti Ferreira [UNESP] |
dc.subject.por.fl_str_mv |
caco-2 cells colon-targeted release everted gut sac in vitro permeability Polymeric nanoparticles triple co-culture model |
topic |
caco-2 cells colon-targeted release everted gut sac in vitro permeability Polymeric nanoparticles triple co-culture model |
description |
Nanoparticles based on gellan gum/pectin blends were designed for colon-targeted release of resveratrol (RES). Their impact on drug release rates and permeability were evaluated using Caco-2 cell model and mucus secreting triple co-culture model. Polymeric nanoparticles (PNP) were successfully prepared by nebulization/ionotropic gelation, achieving high drug loading (>80%). PNP were spherical with a low positive charge density (+5mV) and exhibited diameters of around 330 nm. Developed PNP were able to promote effective modulation of drug release rates, so that only 3% of RES was released in acidic media over 2 h, and, in pH 6.8, the drug was released in a sustained manner, reaching 85% in 30 h. The permeability of RES-loaded PNP in the Caco-2 model was 0.15%, while in the triple co-culture model, in the presence of mucus, it reached 5.5%. The everted gut sac experiment corroborated the low permeability of RES-loaded PNP in the presence or absence of mucus and highlighted their high ability to interact with the intestinal tissue. Results indicate that the novel PNP developed in this work are safe and promising carriers for controlled delivery of RES at the colon. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:34:00Z 2020-12-12T02:34:00Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/03639045.2020.1716374 Drug Development and Industrial Pharmacy, v. 46, n. 2, p. 236-245, 2020. 1520-5762 0363-9045 http://hdl.handle.net/11449/201495 10.1080/03639045.2020.1716374 2-s2.0-85078402703 |
url |
http://dx.doi.org/10.1080/03639045.2020.1716374 http://hdl.handle.net/11449/201495 |
identifier_str_mv |
Drug Development and Industrial Pharmacy, v. 46, n. 2, p. 236-245, 2020. 1520-5762 0363-9045 10.1080/03639045.2020.1716374 2-s2.0-85078402703 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Drug Development and Industrial Pharmacy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
236-245 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128444061450240 |