Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance

Detalhes bibliográficos
Autor(a) principal: Ori, E. L.
Data de Publicação: 2019
Outros Autores: Takagi, E. H., Andrade, T. S., Miguel, B. T., Cergole-Novella, M. C., Guth, B. E. C., Hernandes, R. T. [UNESP], Dias, R. C. B. [UNESP], Pinheiro, S. R. S., Camargo, C. H., Romero, E. C., Dos Santos, L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1017/S0950268818002595
http://hdl.handle.net/11449/184261
Resumo: Diarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies.
id UNSP_e6556a04131f3c46f4dbb79ca6064c2a
oai_identifier_str oai:repositorio.unesp.br:11449/184261
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillanceBacterial infectionsdiarrhoeaEscherichia coli (E. coli)molecular epidemiologysurveillanceDiarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies.Adolfo Lutz Inst Ctr Bacteriol, Natl Reference Lab Escherichia Coil Enter Infect, Sao Paulo, BrazilAdolfo Lutz Inst Ctr Interdisciplinary Procedures, Culture Collect Microorganisms, Sao Paulo, BrazilAdolfo Lutz Inst Reg Lab Santo Andre, Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilCambridge Univ PressAdolfo Lutz Inst Ctr BacteriolAdolfo Lutz Inst Ctr Interdisciplinary ProceduresAdolfo Lutz Inst Reg Lab Santo AndreUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Ori, E. L.Takagi, E. H.Andrade, T. S.Miguel, B. T.Cergole-Novella, M. C.Guth, B. E. C.Hernandes, R. T. [UNESP]Dias, R. C. B. [UNESP]Pinheiro, S. R. S.Camargo, C. H.Romero, E. C.Dos Santos, L. F.2019-10-04T11:56:20Z2019-10-04T11:56:20Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1017/S0950268818002595Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019.0950-2688http://hdl.handle.net/11449/18426110.1017/S0950268818002595WOS:000455339100010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEpidemiology And Infectioninfo:eu-repo/semantics/openAccess2021-10-23T19:28:29Zoai:repositorio.unesp.br:11449/184261Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:22:39.768469Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
title Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
spellingShingle Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
Ori, E. L.
Bacterial infections
diarrhoea
Escherichia coli (E. coli)
molecular epidemiology
surveillance
title_short Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
title_full Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
title_fullStr Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
title_full_unstemmed Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
title_sort Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
author Ori, E. L.
author_facet Ori, E. L.
Takagi, E. H.
Andrade, T. S.
Miguel, B. T.
Cergole-Novella, M. C.
Guth, B. E. C.
Hernandes, R. T. [UNESP]
Dias, R. C. B. [UNESP]
Pinheiro, S. R. S.
Camargo, C. H.
Romero, E. C.
Dos Santos, L. F.
author_role author
author2 Takagi, E. H.
Andrade, T. S.
Miguel, B. T.
Cergole-Novella, M. C.
Guth, B. E. C.
Hernandes, R. T. [UNESP]
Dias, R. C. B. [UNESP]
Pinheiro, S. R. S.
Camargo, C. H.
Romero, E. C.
Dos Santos, L. F.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Adolfo Lutz Inst Ctr Bacteriol
Adolfo Lutz Inst Ctr Interdisciplinary Procedures
Adolfo Lutz Inst Reg Lab Santo Andre
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ori, E. L.
Takagi, E. H.
Andrade, T. S.
Miguel, B. T.
Cergole-Novella, M. C.
Guth, B. E. C.
Hernandes, R. T. [UNESP]
Dias, R. C. B. [UNESP]
Pinheiro, S. R. S.
Camargo, C. H.
Romero, E. C.
Dos Santos, L. F.
dc.subject.por.fl_str_mv Bacterial infections
diarrhoea
Escherichia coli (E. coli)
molecular epidemiology
surveillance
topic Bacterial infections
diarrhoea
Escherichia coli (E. coli)
molecular epidemiology
surveillance
description Diarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T11:56:20Z
2019-10-04T11:56:20Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1017/S0950268818002595
Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019.
0950-2688
http://hdl.handle.net/11449/184261
10.1017/S0950268818002595
WOS:000455339100010
url http://dx.doi.org/10.1017/S0950268818002595
http://hdl.handle.net/11449/184261
identifier_str_mv Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019.
0950-2688
10.1017/S0950268818002595
WOS:000455339100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Epidemiology And Infection
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv Cambridge Univ Press
publisher.none.fl_str_mv Cambridge Univ Press
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128801860747264