Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0950268818002595 http://hdl.handle.net/11449/184261 |
Resumo: | Diarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies. |
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Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillanceBacterial infectionsdiarrhoeaEscherichia coli (E. coli)molecular epidemiologysurveillanceDiarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies.Adolfo Lutz Inst Ctr Bacteriol, Natl Reference Lab Escherichia Coil Enter Infect, Sao Paulo, BrazilAdolfo Lutz Inst Ctr Interdisciplinary Procedures, Culture Collect Microorganisms, Sao Paulo, BrazilAdolfo Lutz Inst Reg Lab Santo Andre, Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilCambridge Univ PressAdolfo Lutz Inst Ctr BacteriolAdolfo Lutz Inst Ctr Interdisciplinary ProceduresAdolfo Lutz Inst Reg Lab Santo AndreUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Ori, E. L.Takagi, E. H.Andrade, T. S.Miguel, B. T.Cergole-Novella, M. C.Guth, B. E. C.Hernandes, R. T. [UNESP]Dias, R. C. B. [UNESP]Pinheiro, S. R. S.Camargo, C. H.Romero, E. C.Dos Santos, L. F.2019-10-04T11:56:20Z2019-10-04T11:56:20Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1017/S0950268818002595Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019.0950-2688http://hdl.handle.net/11449/18426110.1017/S0950268818002595WOS:000455339100010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEpidemiology And Infectioninfo:eu-repo/semantics/openAccess2021-10-23T19:28:29Zoai:repositorio.unesp.br:11449/184261Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:22:39.768469Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
title |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
spellingShingle |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance Ori, E. L. Bacterial infections diarrhoea Escherichia coli (E. coli) molecular epidemiology surveillance |
title_short |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
title_full |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
title_fullStr |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
title_full_unstemmed |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
title_sort |
Diarrhoeagenic Escherichia coli and Escherichia albertii in Brazil: pathotypes and serotypes over a 6-year period of surveillance |
author |
Ori, E. L. |
author_facet |
Ori, E. L. Takagi, E. H. Andrade, T. S. Miguel, B. T. Cergole-Novella, M. C. Guth, B. E. C. Hernandes, R. T. [UNESP] Dias, R. C. B. [UNESP] Pinheiro, S. R. S. Camargo, C. H. Romero, E. C. Dos Santos, L. F. |
author_role |
author |
author2 |
Takagi, E. H. Andrade, T. S. Miguel, B. T. Cergole-Novella, M. C. Guth, B. E. C. Hernandes, R. T. [UNESP] Dias, R. C. B. [UNESP] Pinheiro, S. R. S. Camargo, C. H. Romero, E. C. Dos Santos, L. F. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Adolfo Lutz Inst Ctr Bacteriol Adolfo Lutz Inst Ctr Interdisciplinary Procedures Adolfo Lutz Inst Reg Lab Santo Andre Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ori, E. L. Takagi, E. H. Andrade, T. S. Miguel, B. T. Cergole-Novella, M. C. Guth, B. E. C. Hernandes, R. T. [UNESP] Dias, R. C. B. [UNESP] Pinheiro, S. R. S. Camargo, C. H. Romero, E. C. Dos Santos, L. F. |
dc.subject.por.fl_str_mv |
Bacterial infections diarrhoea Escherichia coli (E. coli) molecular epidemiology surveillance |
topic |
Bacterial infections diarrhoea Escherichia coli (E. coli) molecular epidemiology surveillance |
description |
Diarrhoeagenic Escherichia coli (DEC) is a leading cause of infectious diarrhoea worldwide. In recent years, Escherichia albertii has also been implicated as a cause of human enteric diseases. This study describes the occurrence of E. coli pathotypes and serotypes associated with enteric illness and haemolytic uremic syndrome (HUS) isolated in Brazil from 2011 to 2016. Pathotypes isolated included enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). PCR of stool enrichments for DEC pathotypes was employed, and E. albertii was also sought. O:H serotyping was performed on all DEC isolates. A total of 683 DEC and 10 E. albertii strains were isolated from 5047 clinical samples. The frequencies of DEC pathotypes were 52.6% (359/683) for EPEC, 325% for EAEC, 6.3% for ETEC, 4.4% for ETEC and 4.2% for STEC. DEC strains occurred in patients from 3 months to 96 years old, but EPEC, EAEC and STEC were most prevalent among children. Both typical and atypical isolates of EPEC and EAEC were recovered and presented great serotype heterogeneity. HUS cases were only associated with STEC serotype O157:H7. Two E. albertii isolates belonged to serogroup O113 and one had the stx2f gene. The higher prevalence of atypical EPEC in relation to EAEC in community-acquired diarrhoea in Brazil suggests a shift in the trend of DEC pathotypes circulation as previously EAEC predominated. This is the first report of E. albertii isolation from active surveillance. These results highlight the need of continuing DEC and E. albertii surveillance, as a mean to detect changes in the pattern of pathotypes and serotypes circulation and provide useful information for intervention and control strategies. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T11:56:20Z 2019-10-04T11:56:20Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0950268818002595 Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019. 0950-2688 http://hdl.handle.net/11449/184261 10.1017/S0950268818002595 WOS:000455339100010 |
url |
http://dx.doi.org/10.1017/S0950268818002595 http://hdl.handle.net/11449/184261 |
identifier_str_mv |
Epidemiology And Infection. New York: Cambridge Univ Press, v. 147, 9 p., 2019. 0950-2688 10.1017/S0950268818002595 WOS:000455339100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Epidemiology And Infection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128801860747264 |