Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2016.12.018 http://hdl.handle.net/11449/178724 |
Resumo: | Introduction Mineral trioxide aggregate (MTA) has excellent biological properties, but its handling properties have been criticized for both ProRoot MTA (Tulsa Dental Products, Tulsa, OK) and white MTA-Angelus (MTA-Ang; Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brazil). Angelus MTA HP (high plasticity) (Angelus Indústria de Produtos Odontológicos S/A) has been introduced recently. Considering the importance of biological properties of materials that will be in contact with the tissues, this study evaluated the cytotoxicity, biocompatibility, and biomineralization of MTA HP compared with white MTA-Ang. Methods L929 fibroblast cell lines were cultured, and cell viability was assessed at 6, 24, 48, and 72 hours using the alamar Blue assay (Thermo Fisher Scientific, Waltham, MA). A subcutaneous implant test was performed with polyethylene tubes containing 1 of the materials or empty tubes (control) using 20 Wistar rats. After 7 and 30 days of implantation, the tubes with surrounding tissues were removed for analysis using hematoxylin-eosin or von Kossa stain or they remained unstained for observation under polarized light. The results were statistically analyzed (P < .05). Results A significant increase in cell viability for MTA HP was observed after 24, 48, and 72 hours compared with the control (P < .05). At 72 hours, MTA HP exhibited a higher viability compared with white MTA-Ang (P < .05). Histologic analysis performed at 7 days showed moderate inflammation and a thick fibrous capsule in all groups (P > .05). At 30 days, mild inflammation and a thin fibrous capsule were observed in all groups (P > .05). All materials had structures positive for von Kossa and birefringent to polarized light. Conclusions MTA HP showed biocompatibility and biomineralization similar to MTA-Ang. In addition, MTA HP showed increased fibroblast cell viability compared with white MTA-Ang after a longer period. |
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Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA MaterialBiocompatibilitybiomineralization abilitycytotoxicitymineral trioxide aggregateIntroduction Mineral trioxide aggregate (MTA) has excellent biological properties, but its handling properties have been criticized for both ProRoot MTA (Tulsa Dental Products, Tulsa, OK) and white MTA-Angelus (MTA-Ang; Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brazil). Angelus MTA HP (high plasticity) (Angelus Indústria de Produtos Odontológicos S/A) has been introduced recently. Considering the importance of biological properties of materials that will be in contact with the tissues, this study evaluated the cytotoxicity, biocompatibility, and biomineralization of MTA HP compared with white MTA-Ang. Methods L929 fibroblast cell lines were cultured, and cell viability was assessed at 6, 24, 48, and 72 hours using the alamar Blue assay (Thermo Fisher Scientific, Waltham, MA). A subcutaneous implant test was performed with polyethylene tubes containing 1 of the materials or empty tubes (control) using 20 Wistar rats. After 7 and 30 days of implantation, the tubes with surrounding tissues were removed for analysis using hematoxylin-eosin or von Kossa stain or they remained unstained for observation under polarized light. The results were statistically analyzed (P < .05). Results A significant increase in cell viability for MTA HP was observed after 24, 48, and 72 hours compared with the control (P < .05). At 72 hours, MTA HP exhibited a higher viability compared with white MTA-Ang (P < .05). Histologic analysis performed at 7 days showed moderate inflammation and a thick fibrous capsule in all groups (P > .05). At 30 days, mild inflammation and a thin fibrous capsule were observed in all groups (P > .05). All materials had structures positive for von Kossa and birefringent to polarized light. Conclusions MTA HP showed biocompatibility and biomineralization similar to MTA-Ang. In addition, MTA HP showed increased fibroblast cell viability compared with white MTA-Ang after a longer period.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Endodontics São Paulo State University (Unesp) School of DentistryDepartment of Basic Science São Paulo State University (Unesp) School of DentistryDepartment of Endodontics São Paulo State University (Unesp) School of DentistryDepartment of Basic Science São Paulo State University (Unesp) School of DentistryCNPq: 305969/2015-3Universidade Estadual Paulista (Unesp)Cintra, Luciano Tavares Angelo [UNESP]Benetti, Francine [UNESP]de Azevedo Queiroz, Índia Olinta [UNESP]de Araújo Lopes, Juliana Maria [UNESP]Penha de Oliveira, Sandra Helena [UNESP]Sivieri Araújo, Gustavo [UNESP]Gomes-Filho, João Eduardo [UNESP]2018-12-11T17:31:50Z2018-12-11T17:31:50Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article774-778application/pdfhttp://dx.doi.org/10.1016/j.joen.2016.12.018Journal of Endodontics, v. 43, n. 5, p. 774-778, 2017.0099-2399http://hdl.handle.net/11449/17872410.1016/j.joen.2016.12.0182-s2.0-850157124452-s2.0-85015712445.pdf9235743081667362Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodontics1,585info:eu-repo/semantics/openAccess2024-09-19T18:31:13Zoai:repositorio.unesp.br:11449/178724Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
title |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
spellingShingle |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material Cintra, Luciano Tavares Angelo [UNESP] Biocompatibility biomineralization ability cytotoxicity mineral trioxide aggregate |
title_short |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
title_full |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
title_fullStr |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
title_full_unstemmed |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
title_sort |
Cytotoxicity, Biocompatibility, and Biomineralization of the New High-plasticity MTA Material |
author |
Cintra, Luciano Tavares Angelo [UNESP] |
author_facet |
Cintra, Luciano Tavares Angelo [UNESP] Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] de Araújo Lopes, Juliana Maria [UNESP] Penha de Oliveira, Sandra Helena [UNESP] Sivieri Araújo, Gustavo [UNESP] Gomes-Filho, João Eduardo [UNESP] |
author_role |
author |
author2 |
Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] de Araújo Lopes, Juliana Maria [UNESP] Penha de Oliveira, Sandra Helena [UNESP] Sivieri Araújo, Gustavo [UNESP] Gomes-Filho, João Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cintra, Luciano Tavares Angelo [UNESP] Benetti, Francine [UNESP] de Azevedo Queiroz, Índia Olinta [UNESP] de Araújo Lopes, Juliana Maria [UNESP] Penha de Oliveira, Sandra Helena [UNESP] Sivieri Araújo, Gustavo [UNESP] Gomes-Filho, João Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatibility biomineralization ability cytotoxicity mineral trioxide aggregate |
topic |
Biocompatibility biomineralization ability cytotoxicity mineral trioxide aggregate |
description |
Introduction Mineral trioxide aggregate (MTA) has excellent biological properties, but its handling properties have been criticized for both ProRoot MTA (Tulsa Dental Products, Tulsa, OK) and white MTA-Angelus (MTA-Ang; Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brazil). Angelus MTA HP (high plasticity) (Angelus Indústria de Produtos Odontológicos S/A) has been introduced recently. Considering the importance of biological properties of materials that will be in contact with the tissues, this study evaluated the cytotoxicity, biocompatibility, and biomineralization of MTA HP compared with white MTA-Ang. Methods L929 fibroblast cell lines were cultured, and cell viability was assessed at 6, 24, 48, and 72 hours using the alamar Blue assay (Thermo Fisher Scientific, Waltham, MA). A subcutaneous implant test was performed with polyethylene tubes containing 1 of the materials or empty tubes (control) using 20 Wistar rats. After 7 and 30 days of implantation, the tubes with surrounding tissues were removed for analysis using hematoxylin-eosin or von Kossa stain or they remained unstained for observation under polarized light. The results were statistically analyzed (P < .05). Results A significant increase in cell viability for MTA HP was observed after 24, 48, and 72 hours compared with the control (P < .05). At 72 hours, MTA HP exhibited a higher viability compared with white MTA-Ang (P < .05). Histologic analysis performed at 7 days showed moderate inflammation and a thick fibrous capsule in all groups (P > .05). At 30 days, mild inflammation and a thin fibrous capsule were observed in all groups (P > .05). All materials had structures positive for von Kossa and birefringent to polarized light. Conclusions MTA HP showed biocompatibility and biomineralization similar to MTA-Ang. In addition, MTA HP showed increased fibroblast cell viability compared with white MTA-Ang after a longer period. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2018-12-11T17:31:50Z 2018-12-11T17:31:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2016.12.018 Journal of Endodontics, v. 43, n. 5, p. 774-778, 2017. 0099-2399 http://hdl.handle.net/11449/178724 10.1016/j.joen.2016.12.018 2-s2.0-85015712445 2-s2.0-85015712445.pdf 9235743081667362 |
url |
http://dx.doi.org/10.1016/j.joen.2016.12.018 http://hdl.handle.net/11449/178724 |
identifier_str_mv |
Journal of Endodontics, v. 43, n. 5, p. 774-778, 2017. 0099-2399 10.1016/j.joen.2016.12.018 2-s2.0-85015712445 2-s2.0-85015712445.pdf 9235743081667362 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics 1,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
774-778 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546373981667328 |